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Epidemiological characteristics of pulmonary pneumocystosis and concurrent infections in pigs in Jeju Island, Korea
Ki-Seung Kim, Ji-Youl Jung, Jae-Hoon Kim, Sang-Chul Kang, Eui-Kyung Hwang, Bong-Kyun Park, Dae-Yong Kim, Jae-Hoon Kim* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 3 15-19 (5 pages)
Epidemiological characteristics of swine pulmonary Pneumocystis (P.) carinii and concurrent infections were surveyed on Jeju Island, Korea, within a designated period in 172 pigs submitted from 54 farms to the Department of Veterinary Medicine, Jeju National University. The submitted cases were evaluated by histopathology, immunohistochemistry, PCR/RT-PCR, and bacteriology. P. carinii infection was confirmed in 39 (22.7%) of the 172 pigs. Histopathologically, the lungs had moderate to severe... -
Novel insertion mutation of ABCB1 gene in an ivermectin-sensitive Border Collie
Jae-Ik Han, Hyoung-Won Son, Seung-Cheol Park, Ki-Jeong Na* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 10 341-344 (4 pages)
P-glycoprotein (P-gp) is encoded by the ABCB1 gene and acts as an efflux pump for xenobiotics. In the Border Collie, a nonsense mutation caused by a 4-base pair deletion in the ABCB1 gene is associated with a premature stop to P-gp synthesis. In this study, we examined the full- length coding sequence of the ABCB1 gene in an ivermectin- sensitive Border Collie that lacked the aforementioned deletion mutation. The sequence was compared to the corresponding sequences of a wild-type Beagle and... -
Sequence and phylogenetic analysis of the gp200 protein of Ehrlichia canis from dogs in Taiwan
Chia-Chia Huang, Yu-Chen Hsieh, Chau-Loong Tsang, Yang-Tsung Chung* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 9 333-340 (8 pages)
Ehrlichia (E.) canis is a Gram-negative obligate intracellular bacterium responsible for canine monocytic ehrlichiosis. Currently, the genetic diversity of E. canis strains worldwide is poorly defined. In the present study, sequence analysis of the nearly full-length 16S rDNA (1,620 bp) and the complete coding region (4,269 bp) of the gp200 gene, which encodes the largest major immunoreactive protein in E. canis, from 17 Taiwanese samples was conducted. The resultant 16S rDNA sequences were... -
Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties
H. ?lbegi-Mohyla, M. Hijazin, J. Alber, C. L?mmler, A. A. Hassan, A. Abdulmawjood, E. Prenger-Berninghoff, R. Weiß, M. Zsch?ck 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 13 265-267 (3 pages)
of RNA polymerase encoding gene rpoB, of both strains and of reference strains representing nine species of the genus Arcanobacterium. The species identity of the two A. pyogenes strains could additionally be confirmed by PCR mediated amplification of species specific parts of the 16S-23S rDNA intergenic spacer region, the pyolysin encoding gene plo and by amplification of the collagen-binding protein encoding gene cbpA. All these molecular targets might help to improve the future identification... -
Sequence and phylogenetic analysis of the non-structural 3A and 3B protein-coding regions of foot-and-mouth disease virus subtype A Iran 05
Saber Jelokhani-Niaraki, Majid Esmaelizad*, Morteza Daliri, Rasoul Vaez-Torshizi, Morteza Kamalzadeh, Mohsen Lotfi 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 10 243-247 (5 pages)
The A Iran 05 foot-and-mouth disease virus (FMDV) subtype was detected in Iran during 2005 and has proven to be highly virulent. This study was undertaken to focus on molecular and phylogenetic analysis of 3A and 3B coding-regions in the A Iran 05 field isolate. To assess the genetic relatedness of A Iran 05 isolate the nucleotide and predicted amino acid sequences of the 3AB region of type A FMDV isolates were compared with twenty previously described type A FMDV isolates. The phylogenetic tree... -
Prevalence of tick-borne encephalitis virus in ticks from southern Korea
Sungjin Ko, Jun-Gu Kang, Su Yeon Kim, Heung-Chul Kim, Terry A. Klein, Sung-Tae Chong, William J. Sames, Seok-Min Yun, Young-Ran 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 4 197-203 (7 pages)
The prevalence of tick-borne encephalitis virus (TBEV) in southern Korea was determined by collecting ticks using tick drags. A total of 4,077 of 6,788 ticks collected were pooled (649 pools) according to collection site, species, and developmental stage and assayed for TBEV. The TBEV protein E and NS5 gene fragments were detected using RT-nested PCR in six pools of nymphs collected from Jeju Island (2,491 ticks). The minimum field detection rates for TBEV were 0.17% and 0.14% for Haemaphysalis... -
Production of specific antibodies against SARS-coronavirus nucleocapsid protein without cross reactivity with human coronaviruses 229E and OC43
Hyun-Kyoung Lee, Byoung-Hee Lee, Seung-Hyeok Seok, Min-Won Baek, Hui-Young Lee, Dong-Jae Kim, Yi-Rang Na, Kyoung-Jin Noh, Sung-H 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 12 165-167 (3 pages)
Severe acute respiratory syndrome (SARS) is a life-threatening disease for which accurate diagnosis is essential. Although many tools have been developed for the diagnosis of SARS, false-positive reactions in negative sera may occur because of cross-reactivity with other coronaviruses. We have raised polyclonal and monoclonal antibodies (Abs) using a recombinant form of the SARS virus nucleocapsid protein. Cross-reactivity of these anti-SARS Abs against human coronavirus (HCoV) 229E and HCoV... -
Molecular identification of the vaccine strain from the inactivated oil emulsion H9N2 low pathogenic avian influenza vaccine
Jun-Gu Choi, Youn-Jeong Lee, Ji-Yeon Kim, Yeon-Hee Kim, Mi-Ra Paek, Dong-Kun Yang, Seong-Wan Son, Jae-Hong Kim 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 11 161-163 (3 pages)
In order to control the H9N2 subtype low pathogenic avian influenza (LPAI), an inactivated vaccine has been used in Korea since 2007. The Korean veterinary authority permitted the use of a single H9N2 LPAI vaccine strain to simplify the evolution of the circulating virus due to the immune pressure caused by the vaccine use. It is therefore important to determine the suitability of the vaccine strain in the final inactivated oil emulsion LPAI vaccine. In this study, we applied molecular rather... -
Induction of apoptotic lesions in liver and lymphoid tissues and modulation of cytokine mRNA expression by acute exposure to deoxynivalenol in piglets
Osamu Mikami, Hiroyuki Yamaguchi, Hideo Murata, Yasuyuki Nakajima, Shigeru Miyazaki 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 3 107-113 (7 pages)
systemic apoptosis of lymphocytes in lymphoid tissues and hepatocytes. Apoptosis of lymphocytes and hepatocytes was confirmed by the TdT-mediated dUTP-biotin nick end-labeling (TUNEL) method and immunohistochemical staining against single- stranded DNA and cleaved caspase-3. The number of TUNEL- positive cells in the thymus and Peyer's patches of the ileum was increased at 24 h PI compared to 6 h PI, but the peak was at 6 h PI in the liver. The mRNA expression of interleukin (IL)-1b, IL-6, IL-1... -
Isolation and characterization of avian metapneumovirus from chickens in Korea
Ji-Sun Kwon, Hyun-Jeong Lee, Seung-Hwan Jeong, Jeong-Yong Park, Young-Ho Hong, Youn-Jeong Lee, Ho-Sik Youn, Dong-Woo Lee4, Sun-H 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 9 59-66 (8 pages)
belonged to subtypes A and B. Two aMPVs subtype A out of the 25 detected aMPVs were isolated by Vero cell passage. In animal experiments with an aMPV isolate, viral RNA was detected in nasal discharge, although no clinical signs of SHS were observed in chickens. In contrast to chickens, turkeys showed severe nasal discharge and a relatively higher titer of viral excretion than chickens. Here, we reveal the co-circulation of aMPV subtypes A and B, and isolate aMPVs from chicken flocks in Korea.


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