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Assessment of Porcine Reproductive and Respiratory Syndrome Virus RNA Load in Sera and Tissues during Acute Infection
Jin-Ho Shin and Thomas W. Molitor 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 4 75-86 (12 pages)
Porcine reproductive and respiratory syndrome virus (PRRSV) RNA load in sera and tissues during acute phase of infection was evaluated using a PCR- based quantitative assay. More than 80% of infected pigs (21/25) showed the peak level of viral RNA concentrations in serum (up to 8.6 x 10(8) copies/ml) at day 5 postinfection (PI), and started to clear the virus from the systemic circulation thereafter. Regression analysis using the viral RNA concentrations in sera obtained from days 5 to 14 PI... -
Assessment of Replication and Virulence of Attenuated Pseudorabies Virus in Swine
T. J. Newby, D. P. Carter, K.-J. Yoon, M. W. Jackwood and P. A. Hawkins 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 1 61-66 (6 pages)
A nonclinical study was conducted to characterize the replication behavior of a modified live gE-deleted pseudorabies virus (PRV MS) in swine and potential for reversion to virulence after animal passages. Two to 3 week-old weaned pigs, negative for PRV, were maintained in isolation and challenged by intranasal instillation. For the first passage, 6 pigs were given 1 mL of PRV MS (10(7.3)TCID(50)/mL) and 2 were necropsied at 3, 4 and 5 days post-inoculation (PI). Brain and secondary lymphoid... -
Effective methods for the production of immunoglobulin Y using immunogens of Bordetella bronchiseptica , Pasteurella multocida and Actinobacillus pleuropneumoniae
Jong Man Kim, Han Sang Yoo, In Soo Choi, Won Hur, Na Ri Shin 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 8 47-57 (11 pages)
Swine respiratory diseases induce severe economic losses in the swine industry worldwide. Several methods have been developed and applied to control these diseases. However, there are still problems of disease control in the swine industry. Recently, egg yolk antibodies have been found to offer several advantages for disease control in animals and humans. In a previous study (24), antibodies to several causative pathogens of swine respiratory diseases were developed. However, several problems... -
Additive Estrogenic Activities of the Binary Mixtures of Four Estrogenic Chemicals in Recombinant Yeast Expressing Human Estrogen Receptor
Yong Soon Lee, Kyung Sun Kang, Sung Dae Cho 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 1 1-5 (5 pages)
To evaluate the estrogenic activities of several chemicals such as 17beta-estradiol (E2), rho-nonylphenol, bisphenol A, butylparaben, and combinations of these chemicals, we used recombinant yeasts containing the human estrogen receptor [Saccharomyces cerevisiae ER + LYS 8127]. We evaluated E2 was most active in the recombinant yeast assay, followed by rho-nonylphenol, bisphenol A, butylparaben. The combinations of some concentrations of 17-estradiol as a strong estrogen and bisphenol A or... -
Angiogenesis effects of nerve growth factor (NGF) on rat corneas
Kangmoon Seo, Jongil Choi, Myungjin Park, Changhun Rhee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 10 125-130 (6 pages)
5.0 were higher than those of Groups 0 and 0.5 with the passage of time. The number, length, circumferential neovascularization and areas covered by the vessels in Groups 1.0 and 5.0 were significantly more than in Group 0 and Group 0.5 (p<0.05). This study showed that NGF had a dose-dependent angiogenic effects on the rat cornea and that the minimal effective dose of NGF was 1.0 ng per cornea. Also, it showed that NGF would be useful in angiogenic studies as an alternative angiogenic inducer. -
Studies of cocktail therapy with multiple cytokines for neoplasia or infectious disease of the dog I. cDNA cloning of canine IL-3 and IL-6
Il-seob Shin, Hye-ryon Kim, Myung-jin Nam1 and Hwa-young Youn 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 8 115-120 (6 pages)
This paper describes the cloning and sequence analysis of the cDNAs encoding the canine homologues of interleukin-3 (IL-3) and interleukin-6 (IL-6). The coding sequences for canine IL-3 and IL-6 were obtained by using the reverse transcription polymerase chain reaction (RT-PCR) with RNA harvested from canine peripheral blood mononuclear cells (PBMCs). Canine IL-3 cDNA includes a single open reading frame of 432 nucleotides, which encodes a 143 amino acid polypeptide and has 44.7, 42.4, 37 and... -
Tetracycline residue levels in cattle meat from Nairobi salughter house in Kenya
F. K. Muriuki, W. O. Ogara, F. M. Njeruh, E. S. Mitema 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 5 97-101 (5 pages)
Two hundred and fifty beef samples were collected from five slaughterhouses in and around the city of Nairobi. The beef animals were sourced from various parts of the country. Samples of 50-100 grams were collected randomly from the liver, kidney and muscle of different beef carcasses. The samples collected were processed using multiresidue analytical methods that included liquid-gas partitioning and set-pat C18 cartridges chromatographic clean up. Chlortetracycline and oxytetracycline detection... -
Effect of fever on pharmacokinetics and dosage regimen of intramuscularly administered amikacin in goats
A. K. Agrawal, S. D. Singh, C. Jayachandran 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 4 91-96 (6 pages)
higher whereas total body clearance (Cl(B)) was significantly lower in febrile goats as compared to normal goats. Absorption half-life (t(1/2) ka) value differed non-significantly. For maintaining mean therapeutic level of 2 mg/ml, a priming dose (D*) of 14.732.28 mg/kg, followed by maintenance dose (D(o)) of 13.952.28 mg/kg at shorter dosage interval (t) of 8 hr may be useful in case of normal goats whereas lower doses (D* of 13.581.61 mg/kg followed by D(o) of 12.651.60 mg/kg) at longer tau of... -
Comparative study of endocrine cells in the principal pancreatic islets of two teleosts, Silurus asotus (Siluridae) and Siniperca scherzeri (Centropomidae)
Jae-hyun Lee, Sae-kwang Ku, Ki-dae Park and Hyeung-sik Lee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 1 75-80 (6 pages)
in the peripheral regions with low frequency in Siniperca scherzeri. Bovine PPimmunoreactive cells were found in the peripheral region and the mantle zone of the principal pancreatic islets with low and rare frequency, respectively in both species. In conclusion, the regional distribution and relative frequency of endocrine cells in the principal pancreatic islets of Silurus asotus showed general patterns similar to those of other teleostean fishes. But, some speciesdependent distributional... -
Application of pulsed Doppler ultrasound for the evaluation of small intestinal motility in dogs
Yong-joo An, Heechun Lee, Dongwoo Chang, Youngwon Lee, Jai-ki Sung, Mincheol Choi, Junghee Yoon 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 12 71-74 (4 pages)
The purpose of this study was to verify whether small intestinal peristalsis could be observed and quantitatively assessed using pulsed-Doppler ultrasound. Pulsed-Doppler ultrasound was used to evaluate small intestinal peristalsis after a meal in ten normal dogs and ten sedated dogs. The small intestinal peristalses were measured 0, 1, 3, 6, 9, 12, and 24 hours after a 24-hour fast and after feeding. The number of small intestinal peristalsis were 0.133/min, 0.100/min, 0.033/min, 0.167/min,...


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