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Evaluation of glycoproteins purified from adult and larval camel ticks (Hyalomma dromedarii) as a candidate vaccine
Amr E. El Hakim, Yasser E. Shahein*, Sobhy Abdel-Shafy, Amira M. K. Abouelella, Ragaa R. Hamed 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 7 243-249 (7 pages)
In order to identify antigens that can help prevent camel tick infestations, three major glycoproteins (GLPs) about 97, 66 and 40 kDa in size were purified from adult and larval Egyptian ticks, Hyalomma (H.) dromedarii, using a single-step purification method with Con-A sepharose. The purified GLPs were evaluated as vaccines against camel tick infestation in rabbits. The rabbits received three intramuscular inoculations of GLPs (20 mg/animal) on days 0, 14, and 28. In the immunoblot analysis,... -
The critical time of avian leukosis virus subgroup J-mediated immunosuppression during early stage infection in specific pathogen-free chickens
Feng Wang, Xiaowei Wang, Hongbo Chen, Jianzhu Liu, Ziqiang Cheng* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 6 235-241 (7 pages)
The critical time of avian leukosis virus subgroup J (ALV-J)-mediated immunosuppression was determined by body weight, relative immune organ weight, histopathology, and presence of group specific antigen and antibodies in specific pathogen-free (SPF) chickens. CD4 and CD8 cell activity in the spleen, total and differential leukocyte counts in blood, and viral RNA levels in spleen were measured. Significant growth suppression was observed in the two ALV-J-infected groups. A strong immune response... -
Cytotoxicity of gamma-ray in rat immature hippocampal neurons
Miyoung Yang, Myoung-Sub Song, Sung-Ho Kim, Jong-Choon Kim, Joong-Sun Kim, Taekyun Shin, Changjong Moon* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 1 203-207 (5 pages)
This in vitro study evaluated the detrimental effect of acute gamma (g)-irradiation on rat immature hippocampal neurons. Rat immature hippocampal neurons (0.5 day in vitro) were irradiated with 0∼4 Gy g-rays. Cytotoxicity was analyzed using a lactate dehydrogenase release assay at 24 h after g-irradiation. Radiation-induced cytotoxicity in immature hippocampal neurons increased in a dose- dependent manner. Pre-treatments of pro-apoptotic caspase inhibitors and anti-oxidative substances... -
Establishment of a standard operating procedure for predicting the time of calving in cattle
Dominik Streyl, Carola Sauter-Louis, Anna Braunert, Dorothee Lange, Frank Weber, Holm Zerbe 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 11 177-186 (10 pages)
Precise calving monitoring is essential for minimizing the effects of dystocia in cows and calves. We conducted two studies in healthy cows that compared seven clinical signs (broad pelvic ligaments relaxation, vaginal secretion, udder hyperplasia, udder edema, teat filling, tail relaxation, and vulva edema) alone and in combination in order to predict the time of parturition. The relaxation of the broad pelvic ligaments combined with teat filling gave the best values for predicting either... -
Evaluation of the Perkins handheld applanation tonometer in horses and cattle
Silvia Franco Andrade, Daniel Silva Kupper, Luiz Fernando Rodrigues de Pinho, Elizabeth Cunha Franco, Marcus Vin?cius Felix Fabr 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 10 171-176 (6 pages)
The objective of this study was to evaluate and validate the accuracy of the Perkins handheld applanation tonometer for measuring intraocular pressure (IOP) in horses and cattle. Both eyes of 10 adult horses and cattle were evaluated in a postmortem study. The eyes from 10 clinically normal adult horses and cattle were also examined after bilateral auriculopalpebral nerve block and topical anesthesia for an in vivo study. IOP was measured postmortem using direct manometry (measured with an... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
Real time observation of mouse fetal skeleton using a high resolution X-ray synchrotron
Dong Woo Chang, Bora Kim, Jae Hoon Shin, Young Min Yun, Jung Ho Je, Yeu kuang Hwu, Jung Hee Yoon, Je Kyung Seong 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 1 107-114 (8 pages)
The X-ray synchrotron is quite different from conventional radiation sources. This technique may expand the capabilities of conventional radiology and be applied in novel manners for special cases. To evaluate the usefulness of X-ray synchrotron radiation systems for real time observations, mouse fetal skeleton development was monitored with a high resolution X-ray synchrotron. A non-monochromatized X-ray synchrotron (white beam, 5C1 beamline) was employed to observe the skeleton of mice under... -
Effects of alprazolam on capture stress-related serum cortisol responses in Korean raccoon dogs (Nyctereutes procyonoides koreensis)
Sun-A Kim, So-Yeong Lee, Junpei Kimura, Nam-Shik Shin* 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 16 103-105 (3 pages)
The purpose of this study was to evaluate the effect of alprazolam on the stress that Korean raccoon dogs (Nyctereutes procyonoides koreensis) may experience while caught in a live trap by measuring their serum cortisol response. The animals were placed in a live trap with or without being pretreated with oral doses of alprazolam. In both groups, pre-trap blood samples were initially collected without anesthesia before the animals were positioned in the live trap; then post-trap blood samples... -
Computed tomographic evaluation of abdominal fat in minipigs
Jinhwa Chang, Joohyun Jung, Hyeyeon Lee, Dongwoo Chang, Junghee Yoon, Mincheol Choi* 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 13 91-94 (4 pages)
Computed tomography (CT) exams were conducted to determine the distribution of abdominal fat identified based on the CT number measured in Hounsfield Units (HU) and to measure the volume of the abdominal visceral and subcutaneous fat in minipigs. The relationship between the CT-based fat volumes of several vertebral levels and the entire abdomen and anthropometric data including the sagittal abdominal diameter and waist circumference were evaluated. Moreover, the total fat volumes at the T11,... -
Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid
Goo Jang*, So Gun Hong, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 12 83-89 (7 pages)
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the...


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