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31.

A multiplex real-time PCR for differential detection and quantification of Salmonella spp., Salmonella enterica serovar Typhimurium and Enteritidis in meats

Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 7 43-51 (9 pages)

Su Hwa Lee, Byeong Yeal Jung, Nabin Rayamahji, Hee Soo Lee, Woo Jin Jeon, Kang Seuk Choi, Chang Hee Kweon, Han Sang Yoo* 저
...
- 3,000원
a rapid detection system using multiplex real-time polymerase chain reaction (PCR) has been applied for other food-borne pathogens such as Escherichia coli, Staphylococcus aureus and Streptococcus spp. A multiplex real-time PCR was developed for the simultaneous detection of Salmonella spp., especially S. Typhimurium and S. Enteritidis, in beef and pork. For the specific and sensitive multiplex real-time PCR, three representative primers and probes were designed based on sequence data from...
32.

A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 6 35-42 (8 pages)

Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 저
...
- 3,000원
no cross reactivity with other mycobacteria, such as M. avium complex, M. ulcerans, M. marium, M. kansasii, M. abscessus, M. asiaticum, M. phlei, M. fortuitum, M. scrofulaceum, M. intracellulare, M. smegmatis, and M. bovis. The overall assay for the detection of live MAP organisms is comparatively less expensive and quick, especially in comparison to standard MAP detection using a culture method requiring 6-8 weeks of incubation time, and is significantly less expensive than real-time PCR.
33.

Development of immunoassays for the detection of kanamycin in veterinary fields

Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 3 111-117 (7 pages)

Yong Jin, Jin-Wook Jang, Chang-Hoon Han, Mun-Han Lee 저
...
- 3,000원
competitive direct ELISA, the detection limits of kanamycin were determined to be 1.1 ng/ml in PBS, 1.4 ng/ml in plasma, and 1.0 ng/ml in milk. The concentration of intramuscularly injected kanamycin was successfully monitored in rabbit plasma with competitive direct ELISA. Based on the colloidal gold-based immunochromatographic assay, the detection limits of kanamycin were estimated to be about 6-8 ng/ml in PBS, plasma, and milk. The immunochromatographic assay would be suitable for rapid and...
34.

Simultaneous detection of Lawsonia intracellularis, Brachyspira hyodysenteriae and Salmonella spp. in swine intestinal specimens by multiplex polymerase chain reaction

Dong Kyun Suh, Jae Chan Song 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 10 231-237 (7 pages)

Dong Kyun Suh, Jae Chan Song 저
...
- 3,000원
multiplex PCR assay was developed for the simultaneous detection of the etiologic agents associated with porcine proliferative enteropathies (PPE), swine dysentery (SD)and porcine salmonellosis (PS)in a single reaction using DNA from swine intestinal samples. Single and multiplex PCR amplification of DNA from Lawsonia intracellularis, Salmonella typhimurium and Brachyspira hyodysenteriae with each primer set produced fragments of the predicted size without any nonspecific amplification, 210-bp,...
35.

Development of a Lightcycler-based reverse transcription polymerase chain reaction for the detection of foot-and-mouth disease virus

Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Yong Joo Kim, Jee Yong Park, Jong Hyeon Park, Yi Seok Joo, Hee Jong Song 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 3호 6 207-212 (6 pages)

Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Yong Joo Kim, Jee Yong Park, Jong Hyeon Park, Yi Seok Joo, Hee Jong Song 저
...
- 3,000원
primers/probes was developed for the detection of foot-and-mouth disease (FMD)virus. The gene-specific probes labeled fluorogen for the internal ribosomal entry site, Leader sequence and 2B regions were used to detect FMD virus (FMDV). This assay specifically detected FMDV both in cell culture preparations and clinical samples, and was capable of distinguishing FMD from other viral diseases similar to clinical signs (swine vesicular disease, vesicular stomatitis and bovine viral diarrhea). This...
36.

Development of a sandwich ELISA for the detection of Listeria spp. using specific flagella antibodies

Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 1호 6 41-46 (6 pages)

Seong-Hee Kim, Min-Keun Park, Jin-Young Kim, Pham Duc Chuong, Yong-Soon Lee, Byoung-Su Yoon, Kyu-Kye Hwang, Yoon- Kyu Lim 저
...
- 3,000원
the developed ELISA have been analyzed. The detection limit of ELISA using MAb 2B1 and HRP labeled IgY was 1 ×105cells/0.1 ml at 22℃ and 1×106 cells/0.1 ml at 30℃. ELISA using the pair of MAbs (MAbs 2B1 and HRP labeled MAbs 7A3) detected up to 104cells/0.1 ml at 22℃ and 30℃. Detection limit of sandwich ELISA using IgY was 10 times lower than MAb pair. Using the developed ELISA, we could detect several Listeria contaminated in food samples after 48 h-culturing. In conclusion, both MAbs and...
37.

TaqMan reverse transcription polymerase chain reaction for the detection of Japanese encephalitis virus

Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Seong-Hee Kim, Jun-Hun Kwon, Hong-Ryul Han 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 10 345-351 (7 pages)

Dong-Kun Yang, Chang-Hee Kweon, Byoung-Han Kim, Seong-In Lim, Seong-Hee Kim, Jun-Hun Kwon, Hong-Ryul Han 저
...
- 3,000원
One step TaqMan reverse transcription polymerase chain reaction (RT-PCR) using TaqMan probe was developed for detection of Japanese encephalitis virus (JEV). Real-time RT-PCR was optimized to quantify JEV using the detection system (Rotor Gene 2000 detector) and dual-labeled fluorogenic probes. The gene specific labeled fluorogenic probe for the 3' non-translated region (3' NTR) was used to detect JEV. When the specificity of the assay using specific JEV primers was evaluated by testing three...
38.

Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections

Okjin Kim 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 10 71-73 (3 pages)

Okjin Kim 저
...
- 1,500원
fields. Sections of the CHV-infected cell preparation were subjected to several different ISH protocols using digoxigenin- or biotin-labeled probe respectively. Results were compared for the hybridization and background signal intensities. The best result was obtained by the optimized ISH protocol using digoxigenin-labeled probe for detection of CHV DNA. The optimized ISH assay, which developed in this study, may be a valid tool for the study of pathogenesis and diagnosis of CHV infection.
39.

Detection of Cadmium in Mineral Salt Commercial Mixturesfor Beef Cattle

Wilmar Sachetin Marcal, Paulo Eduardo Pardo, Marcos Roberto Lopes do Nascimento, Marcio Liboni and Marcio de Nadai Bonin 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 12 141-144 (4 pages)

Wilmar Sachetin Marcal, Paulo Eduardo Pardo, Marcos Roberto Lopes do Nascimento, Marcio Liboni and Marcio de Nadai Bonin 저
...
- 1,500원
The cadmium concentrations in mineral mixtures used in beef cattle feed in the states of Sao Paulo and Parana, Brazil was measured. The cadmium concentration was determined by inductively coupled plasma atomic emission spectrometry. Of the 36 analyzed samples, 35 had values > 0.5 ppm (range 0.5 to 11.2 ppm), which is the maximum concentration recommended [4, 18]. These findings show the necessity for careful industrial monitoring, as some mineral mixtures contain sufficient cadmium to cause...
40.

Detection of Lawsonia intracellularis in diagnostic specimens by one-step PCR

Dong-Kyun Suh, Suk-Kyung Lym, You-Chan Bae, Keun-Woo Lee, Won-Pil Choi, Jae-Chan Song 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 5 33-37 (5 pages)

Dong-Kyun Suh, Suk-Kyung Lym, You-Chan Bae, Keun-Woo Lee, Won-Pil Choi, Jae-Chan Song 저
...
- 1,500원
clinical diagnostic method was developed for the rapid detection of porcine proliferative enteritis (PPE) caused by L. intracellularis. Primers were designed based on the p78 DNA clone of L. intracellularis. The one step PCR resulted in the formation of a specific 210-bp DNA product derived from L. intracellularis. The nonspecific amplification product was not detected with swine genomic DNA or other bacterial strains causing similar symptoms to L. intracellularis infection. The one step PCR was...

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