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Evaluation of a competitive ELISA for antibody detection against avian influenza virus
Dae Sub Song, Youn Jeong Lee, Ok Mi Jeong, Yong Joo Kim, Chan Hee Park, Jung Eun Yoo, Woo Jin Jeon, Jun Hun Kwon, Gun Woo Ha, Bo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 8 323-329 (7 pages)
Active serologic surveillance is necessary to control the spread of the avian influenza virus (AIV). In this study, we evaluated a commercially-available cELISA in terms of its ability to detect AIV antibodies in the sera of 3,358 animals from twelve species. cELISA detected antibodies against reference H1- through H15-subtype AIV strains without cross reactivity. Furthermore, the cELISA was able to detect antibodies produced following a challenge of the AIV H9N2 subtype in chickens, or... -
A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen
Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 11 249-255 (7 pages)
The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The... -
Stereolithographic biomodeling of equine ovary based on 3D serial digitizing device
Junpei Kimura*, Nobunori Kakusho, Kenji Yamazawa, Yuuko Hirano, Yasuo Nambo, Hideo Yokota, Ryutaro Himeno 대한수의학회 Journal of Veterinary Science 3 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 11 161-163 (3 pages)
The 3D internal structure microscopy (3D-ISM) was applied to the equine ovary, which possesses peculiar structural characteristics. Stereolithography was applied to make a life-sized model by means of data obtained from 3D-ISM. Images from serially sliced surfaces contributed to a successful 3D reconstruction of the equine ovary. Photopolymerized resin models of equine ovaries produced by stereolithography can clearly show the internal structure and spatial localizations in the ovary. The... -
Comparative study on the aflatoxin B1 degradation ability of rumen fluid from Holstein steers and Korean native goats
Santi Devi Upadhaya, Ha Guyn Sung, Chan Hee Lee, Se Young Lee, Sun Woo Kim, Kyung Jin Cho, Jong K. Ha* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 5 29-34 (6 pages)
The aflatoxin B1 degrading abilities of two different ruminants were compared in this study. One set of experiments evaluated the aflatoxin B1 degradation ability of different rumen fluid donors (steers vs. goats) as well as the rumen fluid filtration method (cheese cloth filtered vs. 0.45 Ռm Millipore) in a 2 × 2 factorial arrangement. Additional studies examined aflatoxin B1 degradation by collecting rumen fluid at different times (0, 3, 6, 9 and 12 h) after feeding. Cannulated... -
Efficacy of nano-hydroxyapatite prepared by an aqueous solution combustion technique in healing bone defects of goat
Samit Kumar Nandi, Biswanath Kundu*, Samir Kumar Ghosh, Dipak Kumar De, Debabrata Basu 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 9 183-192 (10 pages)
Histologic methods showed complete normal ossification with development of Haversian canals and well-defined osteoblasts at the periphery in group II, whereas the control group had moderate fibro-collagenization and an adequate amount of marrow material, fat cells, and blood vessels. An oxytetracycline labeling study showed moderate activity of new bone formation with crossing-over of new bone trabeculae along with the presence of resorption cavities in group II, whereas in the control group,... -
The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain
Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 10 75-83 (9 pages)
An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7,... -
Passive immunization using purified IgYs against infectious bursal disease of chickens in Pakistan
Muhammad Wasif Malik, Najma Ayub, Irfan Zia Qureshi 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 7 43-46 (4 pages)
Infectious bursal disease (IBD)is an acute and highly contagious disease of young chickens caused by Birnavirus. Mortality of infected birds can be best prevented if injected with antibodies.The present study was an attempt to raise specific hyper-immune polyclonal antibodies against IBD virus in Pakistan.Commercial layers divided into four groups were injected with IBD vaccine subcutaneously according to four different treatment regimens.Eggs were collected daily and antibodies were purified... -
Changes of gastrointestinal argyrophil endocrine cells in the osteoporotic SD rats induced by ovariectomy
Sae-Kwang Ku, Hyeung-Sik Lee, Jae-Hyun Lee 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 3호 1 183-188 (6 pages)
The regional distributions and frequencies of argyrophil endocrine cells in gastrointestinal (GI) tract of osteoporotic Sprague-Dawley rat induced by ovariectomy were studied using Grimelius silver stain. The experimental animals were divided into two groups, one is non-ovariectomized group (Sham) and the other is ovariectomized group (OVX). Samples were collected from each part of GI tract (fundus, pylorus, duodenum, jejunum, ileum, cecum, colon and rectum) at 10th week after ovariectomy or... -
An immunohistochemical Study on the Pancreatic Endocrine Cells of the C57BL/6 Mouse
Sae-Kwang Ku, Hyeung-Sik Lee and Jae-Hyun Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 11 327-333 (7 pages)
The regional distribution and relative frequency of the pancreatic endocrine cells in the C57BL/6 mouse were studied by immunohistochemical method using four types of specific mammalian antisera against insulin, glucagon, somatostatin and human pancreatic polypeptide (PP). The pancreas of mouse could be divided into three portions; pancreatic islets, pancreatic duct and exocrine portions, and pancreatic islets were further subdivided into three regions (central, mantle and peripheral regions)... -
Biological Rhythm in Livestock
Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)
The animal time structure is a basic fact of life, no matter if one wants to study it or not. The time-dependent, mostly rhythmic, and thus to a certain degree predictable, variations of biochemical and physiological functions and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities,...


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