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The role of Bcl-xL and nuclear factor-kB in the effect of taxol on the viability of dendritic cells
Mi-Hyoung Kim, Hong-Gu Joo* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 2 99-103 (5 pages)
Taxol has been used effectively in cancer therapies. Our previous study demonstrated that taxol induced altered maturation and improved viability of dendritic cells (DCs). However, the effects of taxol on DC viability have not been fully elucidated. In the present study, flow cytometric analyses revealed that taxol treatment significantly increased the number of viable DCs and the expression levels of a representative anti-apoptotic protein Bcl-xL. Furthermore, mobilization of the p65 subunit of... -
Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity
Yun-Young Byon, Mi-Hyoung Kim, Eun-Sook Yoo, Kyu-Kye Hwang, Youngheun Jee, Taekyun Shin, Hong-Gu Joo* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 4 359-366 (8 pages)
Fucoidan is a sulfated polysaccharide purified from brown algae including Fucus vesiculosus and has a variety of biological effects including mobilization of hematopoietic progenitor cells. Recently, we demonstrated that fucoidan stimulates the antigen-presenting functions of dendritic cells. In this study, we investigated the radioprotective effects of fucoidan on bone marrow cells (BMCs), which are the main cellular reservoir for the hematopoietic and immune system. To evaluate the effects of... -
Variation in the molecular weight of Photobacterium damselae subsp. piscicida antigens when cultured under different conditions in vitro
Tae S. Jung, Kim D. Thompson, Donatella Volpatti, Marco Galeotti, A. Adams 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 9 255-261 (7 pages)
(Ph. d.) subsp. piscicida, cultured in four different growth media [tryptone soya broth (TSB), glucose-rich medium (GRM), iron-depleted TSB (TSB + IR-), and iron-depleted GRM (GRM + IR-)] was compared by enzyme-linked immunosorbent assay (ELISA) and Western blot analysis using sera obtained from sea bass (Dicentrarchus labrax) raised against live or heat-killed Ph. d. subsp. piscicida. The antigenic expression of Ph. d. subsp. piscicida was found to differ depending on the culture medium used. A... -
Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells
Hyun-Ji Kim, Mi-Hyoung Kim, Yun-Young Byon, Jae Woo Park, Youngheun Jee, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 6 39-44 (6 pages)
An acidic polysaccharide of Panax ginseng (APG), so called ginsan is known to have important immunomodulatory activities. It was recently reported that APG has radioprotective effects in mice but the detailed mechanism was not fully elucidated. This study examined the effects of APG on bone marrow cells (BMs). The phenotypical and functional changes in APG-treated BMs after gamma radiation were studied. The benefit of APG on BMs damaged by gamma radiation was determined by measuring the cell... -
Cellular uptake of magnetic nanoparticle is mediated through energy-dependent endocytosis in A549 cells
Jun-Sung Kim, Tae-Jong Yoon, Kyeong-Nam Yu, Mi Suk Noh, Minah Woo, Byung-Geol Kim, Kee-Ho Lee, Byung-Hyuk Sohn, Seung-Bum Park, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 3 321-326 (6 pages)
Biocompatible silica-overcoated magnetic nanoparticles containing an organic fluorescence dye, rhodamine B isothiocyanate (RITC), within a silica shell [50 nm size, MNP@SiO2(RITC)s] were synthesized. For future application of the MNP@SiO2(RITC)s into diverse areas of research such as drug or gene delivery, bioimaging, and biosensors, detailed information of the cellular uptake process of the nanoparticles is essential. Thus, this study was performed to elucidate the precise mechanism by which... -
Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application
Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 9 337-343 (7 pages)
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6),... -
Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes
Jin Sik Oh, Dae Sub Song, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 12 269-275 (7 pages)
Porcine epidemic diarrhea virus (PEDV) causes an acute enteritis in pigs of all ages, often fatality for neonates. PEDV occupies an intermediate position between two well characterized members of the coronavirus group I, human coronavirus (HCoV-229E) and transmissible gastroenteritis virus (TGEV) which uses aminopeptidase N (APN), a 150 kDa protein, as their receptors. However, the receptor of the PEDV has not been identified yet. A virus overlay protein binding assay (VOPBA) was used to... -
Capsular polysaccharide typing of domestic mastitis-causing Staphylococcus aureus strains and its potential exploration of bovine mastitis vaccine developmen. I. capsular olysaccharide typing, isolation and purification of the strains
Hong-Ryul Han, Son-Il Pak, Seung-Won Kang, Woo-Seog Jong, Cheol-Jong Youn 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 8 53-60 (8 pages)
One hundred seven isolates of Staphylococcus aureus from bovine mastitis were investigated for colony morphology in serum-soft agar (SSA), autoagglutination in salt, and capsular serotype. Capsular polysaccharide (CP) was purified and quantified from the extracts of clinical isolates. Overall, 89 isolates (83.2%) were diffuse in the SSA, without any difference in the proportion of diffuse colony between type 5 and type 8 strains. Some strains exhibited compact colonies in the SSA and expressed... -
Construction of a live attenuated Salmonella strain expressing FanC protein to prevent bovine enterotoxigenic Escherichia coli and evaluation of its immunogenicity in mice
Gayeon Won , Hee Jung Kim , John Hwa Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 1호 2 9-15 (7 pages)
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Fucoidan attenuates 6-hydroxydopamine-induced neurotoxicity by exerting anti-oxidative and anti-apoptotic actions in SH-SY5Y cells
Myung-hwan Kim , Hoon Namgoong , Bae-dong Jung , Myung-sang Kwon , Yeon-shik Choi , Taekyun Shin , Hyoung-chun Kim , Myu 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 1호 1 1-7 (7 pages)
