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31.

Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 3 339-349 (11 pages)

Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 저
...
- 4,000원
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple...
32.

A new recombined porcine reproductive and respiratory syndrome virus virulent strain in China

Jian-guo Dong, Lin-yang Yu, Pei-pei Wang, Le-yi Zhang, Yan-ling Liu, Peng-shuai Liang, Chang-xu Song 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 1호 12 89-98 (10 pages)

Jian-guo Dong, Lin-yang Yu, Pei-pei Wang, Le-yi Zhang, Yan-ling Liu, Peng-shuai Liang, Chang-xu Song 저
...
- 3,000원
Porcine reproductive and respiratory syndrome (PRRS) is one of the most important swine diseases worldwide. In the present study, a new virulent strain of PRRS virus (PRRSV), GDsg, was isolated in Guangdong province, China, and caused high fever, high morbidity, and high mortality in sows and piglets. The genome of this new strain was 15,413 nucleotides (nt) long, and comparative analysis revealed that GDsg shared 82.4% to 94% identity with type 2 PRRSV strains, but only 61.5% identity with type...
33.

Genetic diversity and phylogenetic analysis of porcine reproductive and respiratory syndrome virus in southern China from 2007 to 2014

Qiwen Wu, Zhili Li, Guanqun Zhang, Jianqiang Niu, Xiduo Zeng, Baoli Sun, Jingyun Ma 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 8 317-326 (10 pages)

Qiwen Wu, Zhili Li, Guanqun Zhang, Jianqiang Niu, Xiduo Zeng, Baoli Sun, Jingyun Ma 저
...
- 3,000원
Porcine reproductive and respiratory syndrome virus (PRRSV) has a high degree of genetic variation. In this study, we characterized the genetic variation and evolutionary relationships among circulating PRRSV strains in southern China. We analyzed 29 NSP2 strains and 150 ORF5 strains from clinical samples collected in southern China during 2007?2014. The alignment results showed that the nucleotide identity similarities of the two genes among these strains were 80.5%?99.7% and 80.9%?100%,...
34.

Development of an immunochromatographic strip for detection of antibodies against porcine reproductive and respiratory syndrome virus

Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 7 307-316 (10 pages)

Huawei Li, Jifei Yang, Dengke Bao, Jie Hou, Yubao Zhi, Yanyan Yang, Pengchao Ji, Enmin Zhou, Songlin Qiao, Gaiping Zhang 저
...
- 3,000원
a colloidal gold-labeled recombinant Nsp7 antigen probe was successfully developed for the detection of anti-porcine reproductive and respiratory syndrome virus (PRRSV) antibodies in swine. Recombinant Nsp7 protein of PRRSV labeled with colloidal gold was dispensed on a conjugate pad for use as the detector. Staphylococcal protein A and purified porcine anti-Nsp7 antibodies were blotted on a nitrocellulose membrane to form test and control lines, respectively. A comparison of the strip with...
35.

Survey of porcine respiratory disease complex-associated pathogens among commercial pig farms in Korea via oral fluid method

Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-hyun Cho 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 3호 4 283-289 (7 pages)

Yeotaek Cheong, Changin Oh, Kunkyu Lee, Ki-hyun Cho 저
...
- 3,000원
investigate pathogen-positive rates and correlations among eight pathogens associated with porcine respiratory disease complex by analyzing oral fluid samples from 214 pig groups from 56 commercial farms. Samples collected by a rope-chewing method underwent reverse-transcriptase polymerase chain reaction (RT-PCR) or standard polymerase chain reaction (PCR) analysis, depending on the microorganism. Pathogens were divided into virus and bacteria groups. The former consisted of porcine reproductive...
36.

Duplex nested reverse transcriptase polymerase chain reaction for simultaneous detection of type 2 porcine reproductive and respiratory syndrome virus and porcine circovirus type 2 from tissue samples

Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2017, 제 18권 제 2호 16 253-256 (4 pages)

Hye Kwon Kim, Kwang Soo Lyoo, Thi My Le Huynh, Hyoung Joon Moon, Van Giap Nguyen, Bong Kyun Park 저
...
- 1,500원
There are high levels of co-incidence of porcine reproductive and respiratory syndrome virus (PRRSV) and porcine circovirus type 2 (PCV2) in porcine tissue. This study established a duplex nested reverse transcriptase polymerase chain reaction (RT-PCR) method that targets the genomic RNA of type 2 PRRSV and the mRNA of PCV2 in infected tissues. The method amplified discriminative bands of 347 bp and 265 bp specific for type 2 PRRSV and PCV2, respectively. The limits of detection of the duplex...
37.

Quantitative measurement of influenza virus replication using consecutive bronchoalveolar lavage in the lower respiratory tract of a ferret model

Dong-Hun Lee, Jong-In Kim, Jae-Won Lee, Wook-Hun Chung, Jae-Keun Park, Yu-Na Lee, Jin Soo Han, Hwi-Yool Kim, Sang-Won Lee, Chang 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 14 439-442 (4 pages)

Dong-Hun Lee, Jong-In Kim, Jae-Won Lee, Wook-Hun Chung, Jae-Keun Park, Yu-Na Lee, Jin Soo Han, Hwi-Yool Kim, Sang-Won Lee, Chang 저
...
- 1,500원
The ferret is an established animal model of influenza virus infection. Although viral replication in the upper respiratory tract is usually measured with consecutively collected nasal washes, daily evaluation of viral replication in the lung is limited because a large numbers of ferrets need to be sacrificed at consecutive time points. To overcome this limitation, we performed a virus quantification assay using bronchoalveolar lavage (BAL) fluid. This non-invasive BAL technique allows...
38.

Detection of porcine reproductive and respiratory syndrome virus in oral fluid from naturally infected pigs in a breeding herd

Nguyen Thi Trang, Takuya Hirai*, Tsukasa Yamamoto, Mari Matsuda, Naoko Okumura, Nguyen Thi Huong Giang, Nguyen Thi Lan, Ryoji Ya 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 4 361-367 (7 pages)

Nguyen Thi Trang, Takuya Hirai*, Tsukasa Yamamoto, Mari Matsuda, Naoko Okumura, Nguyen Thi Huong Giang, Nguyen Thi Lan, Ryoji Ya 저
...
- 3,000원
porcine reproductive and respiratory syndrome virus (PRRSV) in naturally infected pigs and to determine whether oral fluid could be used to detect the virus in infected animals. Two sows, seven 2-month-old grower pigs, and 70 6-month-old gilts were included in this study. PRRSV in sera and oral fluid were identified by nested reverse transcription PCR (nRT-PCR) while lung, tonsil, and tissue associated with oral cavity were subjected to nRT-PCR, immunohistochemistry, and in situ hybridization....
39.

Recombinant Kluyveromyces lactis expressing highly pathogenic porcine reproductive and respiratory syndrome virus GP5 elicits mucosal and cell-mediated immune responses in mice

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 5 199-208 (10 pages)

Haiyan Zhao, Yalan Wang, Zhitao Ma, Yongqiang Wang, Wen-hai Feng* 저
...
- 3,000원
Currently, killed-virus and modified-live porcine reproductive and respiratory syndrome virus (PRRSV) vaccines are used to control porcine reproductive and respiratory syndrome. However, both types of vaccines have inherent drawbacks; accordingly, the development of novel PRRSV vaccines is urgently needed. Previous studies have suggested that yeast possesses adjuvant activities, and it has been used as an expression vehicle to elicit immune responses to foreign antigens. In this report,...
40.

Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)

Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 저
...
- 4,000원
porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR). Characteristic fluorescence was observed at different times after pcDNA- ORF5 was expressed in the Marc-145 cells, and PCR products corresponding to...

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