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Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system
Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 15 509-516 (8 pages)
obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery rates with the short disposable needle vs. the long needle were not... -
Feasibility of utilizing the patellar ligament angle for assessing cranial cruciate ligament rupture in dogs
Jung-ha Lee, Soon-wuk Jeong 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 8 563-568 (6 pages)
The patellar ligament angle (PLA) was assessed in 105 normal stifle joints of 79 dogs and 33 stifle joints of 26 dogs with a ruptured cranial cruciate ligament (CrCL). The PLA of stifles with complete CrCL rupture was significantly lower than that of normal stifles, particularly at a flexion angle of 60∼80o in both plain and stress views. If the PLA was ＜90.55o on the stress view with a 60∼80o flexion angle, the dog was diagnosed with a complete rupture of the CrCL with a sensitivity of 83.9%... -
Relationship between pregnancy rate and serum progesterone concentration in cases of porcine embryo transfer
JoonHo Moon, Ji-Yei Choi, Jung-Taek Kang, Sol Ji Park, Su Jin Kim, Goo Jang, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 20 167-171 (5 pages)
The level of P4 at the time of embryo transfer (ET) is important. P4 concentrations and numbers of corpora lutea for 126 recipients were evaluated. Nuclear transfer embryos were transferred into 126 surrogates. 11 maintained their pregnancy until full-term delivery, 17 miscarried, and implantation failed in 98 animals. P4 levels in the full-term group were significantly different from those of the pigs that aborted or in which implantation failed (p ＜ 0.05). However, the numbers of corpora... -
A simplified one-step nuclear transfer procedure alters the gene expression patterns and developmental potential of cloned porcine embryos
Sang Kyu Park, Sangho Roh*, Jong-Im Park* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 8 73-80 (8 pages)
Various somatic cell nuclear transfer (SCNT) techniques for mammalian species have been developed to adjust species-specific procedures to oocyte-associated differences among species. Species-specific SCNT protocols may result in different expression levels of developmentally important genes that may affect embryonic development and pregnancy. In the present study, porcine oocytes were treated with demecolcine that facilitated enucleation with protruding genetic material. Enucleation and donor... -
Development of in vitro produced porcine embryos according to serum types as macromolecule
Jungmin Son, Don Buddika Oshadi Malaweera, Eunsong Lee, Sangtae Shin, Jongki Cho 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 11 315-321 (7 pages)
higher in the PGS group, with no significant differences in the blastocyst formation. However, when GTH was supplemented into the IVM media, there were no significant differences among the four groups in the cleavage rates, development rates of the blastocyst, and cell number of the blastocyst after PA and IVF. In conclusion, PGS is an efficient macromolecule in porcine IVM, and GTH supplementation of the IVM media is beneficial when PS is used as macromolecule, regardless of its origin. -
Quercetin improves the in vitro development of porcine oocytes by decreasing reactive oxygen species levels
Jung-Taek Kang, Dae-Kee Kwon, Sol-Ji Park, Su-Jin Kim, Joon-Ho Moon, Ok-Jae Koo, Goo Jang, Byeong-Chun Lee* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 3 15-20 (6 pages)
ug/mL). However, cleavage rates and blastocyst cell numbers were not affected. Oocytes treated with 1 or 10 mg/mL quercetin had significantly lower (p ＜ 0.05) levels of ROS than the control and group treated with the highest concentration of quercetin (50 ug/mL). Moreover, this highest concentration was detrimental to oocyte nuclear maturation and blastocyst formation. Based on our findings, we concluded that exogenous quercetin reduces ROS levels during oocyte maturation and is beneficial for... -
Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles
Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 9 257-265 (9 pages)
Effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (PFs) was investigated. PFs (200∼250 mm) were isolated by micro-dissection and cultured in 0 (control), 10, 10, 10, and 10 M SNP. To examine the reversible effect of SNP, PFs were cultured with 10 M SNP ＋ 1 mM N-nitro-L-arginine methyl ester (L-NAME) or 1.0 mg hemoglobin (Hb). The results showed that greater concentrations of SNP (10,... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
cell phase and the morula. At 6 h after activation, there was almost no distribution of Ca in the SCNT embryos. However, Ca was distributed in the donor nucleus at 10 h and it was distributed throughout the blastomere in the 2-8 cell embryos. In this study, Ca showed significant fluctuations with regularity of IVF and SCNT groups, but PA did not. Systematic investigation of the Ca location and distribution changes during oocyte maturation and early embryo development processes should facilitate... -
In vitro maturation and fertilization of prepubertal and pubertal black Bengal goat oocytes
Momena Khatun, Mohammad Musharraf Uddin Bhuiyan, Jalal Uddin Ahmed, Aminul Haque, Mohammad Bozlur Rahman*, Mohammed Shamsuddin 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 11 75-82 (8 pages)
Oocytes retrieval, in vitro maturation (IVM) and fertilization (IVF) efficiency are inevitable steps towards in vitro production of embryos. In the present study, these parameters were investigated in the ovaries of prepubertal (n = 31) and pubertal (n = 61) black Bengal goats obtained from a slaughterhouse. Nuclear maturation was evaluated upon aspiration and following IVM in TCM-199 (Earle's salt with L-glutamine and sodium bicarbonate) for 27 h at 39C under 5% CO2 in humidified air. The... -
A comparative study of Sephadex, glass wool and Percoll separation techniques on sperm quality and IVF results for cryopreserved bovine semen
Hae-Lee Lee, Sue-Hee Kim, Dong-Beom Ji, Yong-Jun Kim* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 11 249-255 (7 pages)
The aim of this study was to compare the effects of spermatozoa separation techniques on sperm quality and in-vitro fertilization (IVF) results for cryopreserved bovine semen. Sephadex, glass wool and Percoll gradient separation techniques were used for sperm separation and sperm motility, morphology and membrane integrity were evaluated before and after separation. Also, cleavage and blastocyst developmental rate were investigated after IVF with sperm recovered by each separation technique. The...
