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411.

Topology of Scavenger Receptor Class B Type I (SR-BI) on Brush Border Membrane

Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 4호 3 265-272 (8 pages)

Chang-Hoon Han and Mun-Han Lee 저
...
- 3,000원
Both hydropathy plot and in vitro translation results predict the topology of SR-BI; the receptor is an integral membrane protein of 509 amino acids, consisting of a short cytoplasmic N-terminus of 9 amino acids followed by a first transmembrane domain of 22 amino acids, the extracellular domain of 408 amino acids, the second transmembrane domain of 22 amino acids, and the cytoplasmic C-terminus of 47 amino acids. The immunoblot of rBBMV in the presence or absence of pAb589 peptide antigen (the...
412.

Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)

In-Soo Choi and Han-Sang Yoo 저
...
- 3,000원
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by...
413.

Functional Role of Serine Residues of Transmembrane Dopamin VII in Signal Transduction of CB2 Cannabinoid Receptor

Man-Hee Rhee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 7 185-191 (7 pages)

Man-Hee Rhee 저
...
- 3,000원
Using site-directed mutagenesis technique, I have replaced serine 285 and serine 292 with the alanine, and assessed the binding of agonist and signaling such as the inhibition of adenylyl cyclase activity. I have found that serine 292 has an important role in the signal transduction of cannabinoid agonists, HU-210 and CP55940, but not in that of aminoalkylindoles derivatives WIN55,212-2. All mutants express well in protein level determined by western blot using monoclonal antibody HA 11 as...
414.

SR144528 as Inverse Agonist of CB2 Cannabinoid Receptor

Man-Hee Rhee and Sang-Keun Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 6 179-184 (6 pages)

Man-Hee Rhee and Sang-Keun Kim 저
...
- 3,000원
It is now well established that several G protein- coupled receptors can signal without agonist stimulation (constitutive receptors). Inverse agonists have been shown to inhibit the activity of such constitutive G protein-coupled receptor signaling. Agonist activation of the G(i/o)-coupled peripheral cannabinoid receptor CB2 normally inhibits adenylyl cyclase type V and stimulates adenylyl cyclase type II. Using transfected COS cells, we show here that application of SR144528, an inverse agonist...
415.

Biological Rhythm in Livestock

Piccione Giuseppe and Caola Giovanni 대한수의학회 Journal of Veterinary Science 13 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 1 145-157 (13 pages)

Piccione Giuseppe and Caola Giovanni 저
...
- 4,200원
The animal time structure is a basic fact of life, no matter if one wants to study it or not. The time-dependent, mostly rhythmic, and thus to a certain degree predictable, variations of biochemical and physiological functions and of sensitivity and resistance to many environmental agents are often quite large and offer not only new insight into animal physiology and pathology but also diagnostic possibilities and therapeutic advantages. Chronobiology, chronophysiology and its subspecialities,...
416.

Effect of IP3 and ryanodine treatments on the development of bovine parthenogenetic and reconstructed embryos

Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 2호 11 131-137 (7 pages)

Gook-jun Ahn, Byeong-chun Lee, Woo-suk Hwang 저
...
- 3,000원
For parthenogenetic activation as a model system of nuclear transfer, microinjection and electroporation as activation treatments in bovine metaphase II oocytes were administered to each of three groups as follows: control group (treatments with Ca2+, Mg2+ -free PBS+100 micro M EGTA), IP3 group (control+25 micro M IP3) and IP3+ ryanodine group (control+25 micro M IP3+10 mM ryanodine). In experiments using microinjection, no significant differences were observed between any of the developmental...
417.

Immunohistochemical detection of Prion protein (PrP-Sc) and epidemiological study of BSE in Korea

Hye Cheong Koo, Yong Ho Park, Byeong-Chun Lee, Chanhee Chae, Katherine I. O'Rourke, Timothy V. Baszler 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2001, 제 2권 제 1호 4 25-31 (7 pages)

Hye Cheong Koo, Yong Ho Park, Byeong-Chun Lee, Chanhee Chae, Katherine I. O'Rourke, Timothy V. Baszler 저
...
- 3,000원
Though the aetiology of transmissible spongiform encephalopathies (TSEs) remains uncertain, proteinase resistant prion protein (PrP-Sc), a converted form of the normal cellular prion protein (PrP-C), accumulates in the lysosome of cells of the nervous systems of animals with TSEs. In this study, clinical and epidemiological examinations of bovine spongiform encephalopathy (BSE) were conducted in Korea. During the investigated period, none of the cattle exhibited typical clinical signs of BSE,...
418.

Using pig biliary system, in vivo propagation of Enterocytozoon bieneusi, an AIDS-related zoonotic pathogen

John Hwa Lee 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 7 105-111 (7 pages)

John Hwa Lee 저
...
- 3,000원
A microsporidian parasite Enterocytozoon bieneusi is the most common microorganism recognized in AIDS patients, and slow scientific progress is attributed to our inability to propagate the parasite. We report upon the development of a system of propagation using the pig biliary system. The parasite spores were continuously detected in the bile samples post onset of spore shedding in the gall bladder, which suggests that this organism maintain persistent infection in the biliary system and that...
419.

Immunologic reactivity of a lipopolysaccharide-protein complex of type A Pasteurella multocida in mice

Hyo-ik Ryu, Chul-joong Kim 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 5 87-95 (9 pages)

Hyo-ik Ryu, Chul-joong Kim 저
...
- 3,000원
The immunologic reactivity of a lipopolysaccharide (LPS)-protein complex isolated from a potassium thiocyanate extract of a Pasteurella multocida (capsular type A and somatic type 3) strain was evaluated in mice. The LPS-protein complex provided 100% protection in mice against a challenge with the homologous strain. However, when the complex was fractionated into LPS and protein moieties by phenol-water treatment, both components lacked immunogenicity. The complex and extracted components were...
420.

A study on the immunocytochemical localization of eurofascin in rat sciatic nerve

Byung-joon Chang, Ik-hyun Cho and Peter J. Brophy 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 2호 1 67-71 (5 pages)

Byung-joon Chang, Ik-hyun Cho and Peter J. Brophy 저
...
- 1,500원
We examined the localization of neurofascin (NF) in the sciatic nerve of rat. In the myelinated fibers, neurofascin localizes strongly in the nodal axolemma except the small central cleft and also expresses in the paranodes, and weakly in the Schmidt-Lanterman incisures. In the paranodes, NF localizes around the axolemma and it expresses in the apposing membrane of paranodal loops. Axoplasm, compact myelin and cytoplasm of Schwann cell do not express NF at all. In the Schmidt-Lanterman...

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