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411.

Establishment and identification of cell lines from type O blood Korean native pigs and their efficiency in supporting embryonic development via somatic cell nuclear transfer

Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 4호 3 492-499 (8 pages)

Anukul Taweechaipaisankul, Geon A Kim, Jun-Xue Jin, Su Cheong Yeom, Byeong Chun Lee 저
...
- 3,000원
Due to their similarities with humans in anatomy, physiology, and genetics miniature pigs are becoming an attractive model for biomedical research. We aim to establish and evaluate blood type O cells derived from Korean native pig (KNP), a typical miniature pig breed in Korea. Ten cell lines derived from 8 KNP piglets and one adult female KNP (kidney and ear tissues) were established. To confirm the presence of blood type O, genomic DNA, fucosyltransferase (FUT) expression, and...
412.

Clinical assessment after human adipose stem cell transplantation into dogs

Seok Hee Lee, Erif M.N. Setyawan, Yoo Bin Choi, Jeong Chan Ra, Sung Keun Kang, Byeong Chun Lee, Geon A Kim 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 15 452-461 (10 pages)

Seok Hee Lee, Erif M.N. Setyawan, Yoo Bin Choi, Jeong Chan Ra, Sung Keun Kang, Byeong Chun Lee, Geon A Kim 저
...
- 3,000원
Adipose tissue-derived stem cell (ASCs) are an attractive source of stem cells with therapeutic applicability in various fields for regenerating damaged tissues because of their stemness characteristics. However, little has reported on evaluating adverse responses caused by human ASC therapy. Therefore, in the present study, a clinical assessment after human ASC transplantation into dogs was undertaken. A total of 12 healthy male dogs were selected and divided into four groups: saline infusion,...
413.

Production of transgenic pigs using a pGFAP-CreERT2/EGFP LoxP inducible system for central nervous system disease models

Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 13 434-445 (12 pages)

Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 저
...
- 4,100원
Transgenic (TG) pigs are important in biomedical research and are used in disease modeling, pharmaceutical toxicity testing, and regenerative medicine. In this study, we constructed two vector systems by using the promoter of the pig glial fibrillary acidic protein (pGFAP) gene, which is an astrocyte cell marker. We established donor TG fibroblasts with pGFAP-CreERT2/LCMV-EGFPLoxP and evaluated the effect of the transgenes on TG-somatic cell nuclear transfer (SCNT) embryo development. Cleavage...
414.

Effect of immunization routes and protective efficacy of Brucella antigens delivered via Salmonella vector vaccine

Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 11 416-425 (10 pages)

Jonathan Lalsiamthara, Gayeon Won, John Hwa Lee 저
...
- 3,000원
route-wise comparison of the combined vaccine candidate, intraperitoneal (IP), intramuscular (IM), and subcutaneous immunizations revealed an indication of highly efficient routes of protection. Splenocytes of mice immunized via IM and IP routes showed significant relative expression of IL-17 upon antigenic pulsing. Taken together, each of the Brucella antigens delivered by ST successfully induced an antigen-specific immune response, and it was also evident that an individual antigen strain can...
415.

Prevalence, toxin gene profile, antibiotic resistance, and molecular characterization of Clostridium perfringens from diarrheic and non-diarrheic dogs in Korea

Jung-Whan Chon, Kun-Ho Seo, Dongryeoul Bae, Ji-Hee Park, Saeed Khan, Kidon Sung 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 6 368-374 (7 pages)

Jung-Whan Chon, Kun-Ho Seo, Dongryeoul Bae, Ji-Hee Park, Saeed Khan, Kidon Sung 저
...
- 3,000원
Clostridium perfringens causes diarrhea and other diseases in animals and humans. We investigated the prevalence, toxin gene profiles, and antibiotic resistance of C. perfringens isolated from diarrheic dogs (DD) and non-diarrheic dogs (ND) in two animal hospitals in Seoul, Korea. Fecal samples were collected from clinically DD (n = 49) and ND (n = 34). C. perfringens was isolated from 31 of 49 DD (63.3%) and 21 of 34 ND dogs (61.8%). All C. perfringens strains were positive for the ...
416.

Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang4, Chang 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 5 358-367 (10 pages)

Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang4, Chang 저
...
- 3,000원
The porcine reproductive and respiratory syndrome virus (PRRSV) is a globally ubiquitous swine viral pathogen that causes major economic losses worldwide. We previously reported an over-attenuated phenotype of cell-adapted PRRSV strain CA-2-P100 in vivo. In the present study, CA-2-P100 was serially propagated in cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain the derivative strain CA-2-MP120. Animal inoculation studies revealed that both CA-2-P100 and CA-2-MP120...
417.

Dembo polymerase chain reaction technique for detection of bovine abortion, diarrhea, and respiratory disease complex infectious agents in potential vectors and reservoirs

Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 4 350-357 (8 pages)

Sayed Samim Rahpaya, Shinobu Tsuchiaka, Mai Kishimoto, Mami Oba, Yukie Katayama, Yuka Nunomura, Saki Kokawa, Takashi Kimura, Atsushi Kob 저
...
- 3,000원
Bovine abortion, diarrhea, and respiratory disease complexes, caused by infectious agents, result in high and significant economic losses for the cattle industry. These pathogens are likely transmitted by various vectors and reservoirs including insects, birds, and rodents. However, experimental data supporting this possibility are scarce. We collected 117 samples and screened them for 44 bovine abortive, diarrheal, and respiratory disease complex pathogens by using Dembo polymerase chain...
418.

Genomic characterization and pathogenic study of two porcine reproductive and respiratory syndrome viruses with different virulence in Fujian, China

Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 3 339-349 (11 pages)

Xiukun Sui, Ting Xin, Xiaoyu Guo, Hong Jia, Ming Li, Xintao Gao, Jing Wu, Yitong Jiang, L.Willems, Hongfei Zhu, Shaohua Hou 저
...
- 4,000원
Two strains of porcine reproductive and respiratory syndrome virus (PRRSV) were isolated in 2006 and 2016 and designated as FZ06A and FZ16A, respectively. Inoculation experiments showed that FZ06A caused 100% morbidity and 60% mortality, while FZ16A caused 100% morbidity without death. By using genomic sequence and phylogenetic analyses, close relationships between a Chinese highly pathogenic PRRSV strain and the FZ06A and FZ16A strains were observed. Based on the achieved results, multiple...
419.

Cytological endometritis in dairy cows: diagnostic threshold, risk factors, and impact on reproductive performance

Soo Chan Lee, Jae Kwan Jeong, In Soo Choi, Hyun Gu Kang, Young Hun Jung, Soo Bong Park, Ill Hwa Kim 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 17 301-308 (8 pages)

Soo Chan Lee, Jae Kwan Jeong, In Soo Choi, Hyun Gu Kang, Young Hun Jung, Soo Bong Park, Ill Hwa Kim 저
...
- 3,000원
We determined the threshold proportion of polymorphonuclear leukocytes (PMNs) for a diagnosis of cytological endometritis (CEM), the risk factors for this condition, and its impact on reproductive performance in dairy cows. Uterine cytology was performed on 407 Holstein cows 4 weeks postpartum to determine the proportions of endometrial cells and PMNs. A receiver operator characteristics curve was used to determine the threshold above which the PMN proportion affected the likelihood of cows...
420.

Novel vertebral computed tomography indices in normal and spinal disorder dogs

Jongsu Lim, Youngmin Yoon, Taesung Hwang, Hee Chun Lee 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 16 296-300 (5 pages)

Jongsu Lim, Youngmin Yoon, Taesung Hwang, Hee Chun Lee 저
...
- 1,500원
This study was carried out to derive and evaluate reference computed tomography (CT)-based indices for normal canine spine. CT and magnetic resonance images were acquired from 12 clinically normal Beagle dogs (normal group) and 50 dogs with 56 spinal disorders (patient group). Image acquisition regions were cervical spine (C2-T1), thoracic spine (T1-T13), and lumbar spine (L1-L7). Measured indices were: the ratios of width to height of the spinal cord including the dura matter (CR) and of the...

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