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411.

Research Articles : Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 1 301-309 (9 pages)

저
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- 3,000원
The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination...
412.

Potential Roles of Essential Oils on Controlling Plant Pathogenic Bacteria Xanthomonas Species: A Review

한국식물병리학회 The Plant Pathology Journal 18 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 2 207-224 (18 pages)

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- 4,700원
on the antibacterial efficacy of essential oils that could be considered suitable for application in agriculture as biocontrol measures against plant pathogenic bacteria of Xanthomonas species. The current knowledge on the use of essential oils to control Xanthomonas bacteria in vitro and in vivo models has been discussed. A brief description on the legal aspects on the use of essential oils against bacterial pathogens has also been presented. Through this review, a mode of antibacterial action...
413.

Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)

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- 3,000원
Our previous sequence and phylogenetic analyses of the Korean Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSVRNA segment variations, we developed a reverse transcription- polymerase reaction/restriction enzyme (RTPCR/ RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV...
414.

Identification of Alternaria alternata as a Causal Agent for Leaf Blight in Syringa Species

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 4 120-127 (8 pages)

저
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- 3,000원
While many isolates of Alternaria alternata are common saprophytes on trees and shrubs, this study clearly demonstrated that A. alternata is a primary pathogen in lilac (Syringa sp.), causing a leaf-blight that affects different Syringa species. Isolates of Alternaria sp. were collected from leaf blight samples of lilacs in the field. The internal transcribed spacer (ITS) region and morphological characterization were used to identify lilac blight pathogen. Based on 100% ITS nucleotide sequence...
415.

Detection of Viruses Infecting Stone Fruits in Western Mediterranean Region of Turkey

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 8 44-52 (9 pages)

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- 3,000원
Field surveys were conducted in 45 stone fruit orchards in seven districts of Isparta Province located in western Mediterranean region of Turkey important for stone fruit production. Leaf samples were collected from 175 trees showing virus-like symptoms. These samples were first tested by ELISA for five different RNA viruses including Apple mosaic ilarvirus (ApMV), Prunus necrotic ringspot ilarvirus (PNRSV), Prune dwarf ilarvirus (PDV), Plum pox potyvirus (PPV), Apple chlorotic leafspot...
416.

Greenhouse Method for Assessing Spot Blotch Resistance in Barley

한국식물병리학회 The Plant Pathology Journal 3 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 18 421-423 (3 pages)

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- 1,500원
New sources of barley (Hordeum vulgare L.) resistant to spot blotch, caused by Cochliobolus sativus, are needed to provide effective resistance because of the rapid change pathotype patterns of C. sativus in fields. The purposes of our study were to develop a method to screen barley for resistance to spot blotch disease and then use this methodology to screen barley genotypes for resistance to the major virulent pathotype Pt4 in barley populations in Syria. A transparent tape method, in which a...
417.

Suppression of Green and Blue Mold in Postharvest Mandarin Fruit by Treatment of Pantoea agglomerans 59-4

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 4호 8 353-359 (7 pages)

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- 3,000원
by green and blue mold, was tested for the control efficacy and its mode of action was investigated. Pa 59-4 inhibited infection by green and blue mold on wounded mandarins, which were artificially inoculated with a spore suspension of Penicillium digitatum and P. italicum with control efficacies of 85-90% and 75-80%, respectively. The biocontrol efficacy was increased by raising the concentration of cells to between 108 and 109 cfu/ml, and pretreatment with the antagonist prevented subsequent...
418.

Microwave Irradiation-assisted RNA Extraction from Woody Tissues for Plant Virus Detection

한국식물병리학회 The Plant Pathology Journal 3 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 3호 14 286-288 (3 pages)

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- 1,500원
method, however, is both time-consuming and expensive. Additionally, homogenization may generate excessive endogenous RNases, polyphenolics, and other substances that reduce the quality and quantity of RNA. In this study, we describe the microwave irradiation-assisted RNA extraction (MIRE) technique which, without tissue disruption and homogenization, allows for the cost-effective and rapid generation of intact RNA from apple cane shavings and the reliable detection of apple virus by RT-PCR.
419.

Molecular Characterization and Survey of the Infection Rate of Orchid fleck virus in Commercial Orchids

한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 2호 5 130-138 (9 pages)

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- 3,000원
Orchid fleck virus (OFV) is an unassigned plant virus in the family Rhabdoviridae. OFV was isolated from Cymbidium sp. showing oval necrotic lesions on their leaves in Korea, and designated as OFV-NHHS1. The complete nucleotide sequence of the RNA1 (6,413 nt) (GenBank accession no. AB516442) and RNA2 (6,001 nt) (GenBank accession no. AB516441) was determined in this study. RNA1 and RNA2 contained five and one ORF respectively. RNA1 encodes nucleocapsid (N) of 49 kDa, ORF2 of 26 kDa, ORF3 of 38...
420.

Survey of Egg- and Cyst-parasitic Fungi of Potato Cyst Nematode in Indonesia

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 5 32-36 (5 pages)

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- 1,500원
A hundred percent infections occurred in eggs or cysts by three fungal isolates G. virens, F. oxysporum and P. oxalicum, suggesting that these fungi may have a good potential for the PCN biocontrol. Especially, G. virens isolates, which occurred most frequently in the PCN-infested potato fields and are known to be highly adaptable to varying habitats, may be developed as reliable agents for controlling PCN with both egg- and cyst-parasitic capabilities and with high ecological adaptabilities.

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