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Effect of Endothelin-1 on Proliferation and Differentiation of Rat Tracheal Epithelial Cells
ChangSooKim, SaeOckOh, JaeSukWoo, JinSupJung, YongKeunKim, SangHoLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 13 763-770 (8 pages)
control cells. Endothelin-1 increased mitogen-activated protein kinase (MAPK) activity. Herbimycin A, a tyrosine kinase inhibitor, inhibited endothelin-1-induced proliferation of epithelial cells. The treatment of endothelin-1 during the primary culture of tracheal epithelial cells increased AB-PAS-stained cell population and ciliated cell population 6.5 fold and 1.5 fold, respectively, when compared with those in control cells. The responsiveness to carbachol and forskolin in the Cl- secretion... -
Increase of L-type Calcium Current by cGMP-dependent Protein Kinase Regulates in Rabbit Ventricular Myocytes
JinHan, NariKim, EuiyongKim, WonkyungHo, YungEEarm, HankyounKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 10 733-742 (10 pages)
in rabbit ventricular myocytes using whole-cell voltage clamp method. ICa was elicited by a depolarizing pulse to 10 mV from a holding potential of 40 mV. Extracellular 8-(4-Chlorophenylthio)-guanosine-3',5'-cyclic monophosphate (8-pCPT-cGMP), potent stimulator of cGMP-dependent protein kinase (cGMP-PK), increased basal ICa. cGMP-PK also increased basal ICa. The stimulation of basal ICa by cGMP-PK required both 8-Br-cGMP in low concentration and intracellular ATP to be present. The... -
Sensitivity of Rabbit Cerebral Artery to Serotonin is Increased with the Moderate Increase of Extracellular K+
SukHyoSuh, SungJinPark, JaiYoungChoi, JaeHoonSim, YoungChulKim, SungJoonKim, InsukSo, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.6 6 695-703 (9 pages)
[K]O can be increased under a variety of conditions including subarachnoid hemorrhage. The increase of [K]O in the range of 5∼15 mM may affect tensions of blood vessels and can change their sensitivity to various vasoactive substances. Therefore, it was examined in the present study whether the sensitivity of cerebral arteries to vasoactive substances can be changed with the moderate increase of [K]O, using Mulvany-type myograph and [Ca2]c measurement. The... -
The Effects of Intracellular Monocarboxylates on the ATP-sensitive Potassium Channels in Rabbit Ventricular Myocytes
NariKim, JinHan, Eui-YongKim, Won-KyungHo, YungE.Earm 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 6 581-589 (9 pages)
A regulating mechanism of the ATP-sensitive potassium channels (KATP channels) is yet to fully explained. This study was carried out to investigate the effects of intracellular application of monocarboxylates (acetate, formate, lactate, and pyruvate) on KATP channels in isolated rabbit ventricular myocytes. Single channel currents of KATP channels were recorded using the excised inside-out or permeabilized attached (open-cell) patch-clamp technique at room temperature. Intracellular application... -
Dual Action of d-Tubocurarine on Large-Conductance Ca2+-activated K+ Channels from Rat Brain Reconstituted into Planar Lipid Bilayer
SungkwonChungJungHoonShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 2 549-553 (5 pages)
Using the planar lipid bilayer method, we investigated the effect of d-tubocurarine (dTC) on the extracellular side of large-conductance Ca2+-activated K+ channel from rat brain. When the initial open probability (Po) of the channel was relatively high, dTC decreased channel activity in a concentration dependent manner. In contrast, when the initial Po was lower, sub-micro molar dTC increased channel activity by destabilizing the closed states of the channel. Further addition of dTC up to micro... -
cAMP-Dependent Signalling is Involved in Adenosine-Stimulated Cl Secretion in Rabbit Colon Mucosa
SaeOckOh, EuiYongKim, JinSupJung, JaeSukWoo, YongKeunKim, SangHoLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 14 521-527 (7 pages)
Cl secretion in intestinal epithelial cells still continues. To investigate the effect of adenosine on Cl secretion and its underlying mechanism in the rabbit colon mucosa, we measured short circuit current (ISC) under automatic voltage clamp with DVC-1000 in a modified Ussing chamber. Adenosine, when added to the basolateral side of the muocsa, increased ISC in a dose-dependent manner. The adenosine-stimulated ISC response was abolished when Cl in the bath solution was... -
Mechanism of Vasoactive Intestinal Polypeptide-Induced Catecholamine Secretion from the Rat Adrenal Medulla
Dong-YoonLim, Jae-BongHeo, Cheol-HeeChoi, Geon-HanLim, Yong-GyoonLee, Song-HoonOh, Il-SikKim, Jong-InKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 12 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.4 6 443-454 (12 pages)
The present study was attempted to investigate the effect of vasoactive intestinal polypeptide (VIP) on secretion of catecholamines (CA) and to establish whether there is the existence of a noncholinergic mechanism in adrenomedullary CA secretion from the isolated perfused rat adrenal gland. The perfusion into an adrenal vein of VIP (3⁓106 M) for 5 min or the injection of acetylcholine (ACh, 5.32⁓103 M) resulted in great increases in CA secretion. Tachyphylaxis to... -
Effects of Prostaglandin E2 on the Spontaneous Contractions and Electrical Activities of the Antral Circular Muscle in Guinea-pig Stomach
JongYoonKim, SungJoonKim, TongMookKang, SangJinLee, JaeYeoulJun, InsukSo, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.3 11 361-368 (8 pages)
of control. The frequency of the slows wave was increased from 5.7⁑0.2 cycles/min to 6.5⁑0.2 (n=22). Voltage-operated Ca2 currents were decreased by PGE2 (n=5). Voltage-operated K currents, both Ca-dependent and Ca-independent, were increased (n=5). These results suggest that PGE2 plays an important role in the modulation of gastric smooth muscle activities, and its inhibitory effects on the contractility and activities of slow waves are resulted from both decrease of... -
Comparison of Inodilator Effect of Higenamine, YS49, YS51, Tetrahydroisoquinoline Analogs, and Dobutamine in the Rat
Won-SeogChong, Young-SooLee, Young-JinKang, Duck-HyungLee, Jae-ChunRyu, HyeSookYun-Choi, Ki-ChurlChang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.3 7 323-330 (8 pages)
Tetrahydroisoquinoline (THI) alkaloids can be considered as cyclized derivatives of simple phenylethylamines. Many of them, especially with 6,7-disubstitution, demonstrate a relatively high affinity for catecholamines. Present study examines the pharmacological action of limited series of THI, using rats' isolated atria and aorta. In addition, a [3H] prazosin displacement binding study with THI compounds was performed, using rat brain homogenates to investigate whether these probes have...


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