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Prolonged expression of senescence markers in mice exposed to gamma-irradiation
Min-A Seol, Uhee Jung*, Hyeon Soo Eom, Seol-Hwa Kim, Hae-Ran Park, Sung-Kee Jo 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 1 331-338 (8 pages)
Although ionizing radiation is known to induce cellular senescence in vitro and in vivo, its long-term in vivo effects are not well defined. In this study, we examined the prolonged expression of senescence markers in mice irradiated with single or fractionated doses. C57BL/6 female mice were exposed to 5 Gy of γ-rays in single or 5, 10, 25 fractions. At 2, 4, and 6 months after irradiation, senescence markers including mitochondrial DNA (mtDNA) common deletion, p21, and senescence-associated... -
Protective effects of recombinant Brucella abortus Omp28 against infection with a virulent strain of Brucella abortus 544 in mice
Jeong Ju Lim, Dong Hyeok Kim, Jin Ju Lee, Dae Geun Kim, Wongi Min, Hu Jang Lee, Man Hee Rhee, Suk Kim* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 10 287-292 (6 pages)
The outer membrane proteins (OMPs) of Brucella (B.) abortus have been extensively studied, but their immunogenicity and protective ability against B. abortus infection are still unclear. In the present study, B. abortus Omp28, a group 3 antigen, was amplified by PCR and cloned into a maltose fusion protein expression system. Recombinant Omp28 (rOmp28) was expressed in Escherichia coli and was then purified. Immunogenicity of rOmp28 was confirmed by Western blot analysis with Brucella-positive... -
Pathogen translocation and histopathological lesions in an experimental model of Salmonella Dublin infection in calves receiving lactic acid bacteria and lactose supplements
Laureano S. Frizzo*, Maria V. Zbrun, Lorena P. Soto, Ezequiel Bertozzi, Gabriel J. Sequeira, Luis E. Marti, Marcelo L. Signorini 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 7 261-270 (10 pages)
The purpose of this study was to evaluate the capacity of a lactic acid bacteria (LAB) inoculum to protect calves with or without lactose supplements against Salmonella Dublin infection by evaluating histopathological lesions and pathogen translocation. Fifteen calves were divided into three groups [control group (C-G), a group inoculated with LAB (LAB-G), and a group inoculated with LAB and given lactose supplements (L-LAB-G)] with five, six, and four animals, respectively. The inoculum,... -
Hsp70 and HSF-1 expression is altered in the tissues of pigs transported for various periods of times
Miao Zhang, Zhenhua Yue, Zhijun Liu, Ali Islam, Buriro Rehana, Shu Tang, Endong Bao*, Jorg Hartung 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 6 253-259 (7 pages)
The aim of this study was to assess changes of Hsp70 and HSF-1 protein and mRNA expression in stress-sensitive organs of pigs during transportation for various periods of time. Twenty pigs were randomly divided into four groups (0 h, 1 h, 2 h, and 4 h of transportation). A significant increased activity of AST and CK was observed after 1 h and 2 h of transportation. Histopathological changes in the heart, liver, and stomach indicated that these organs sustained different degrees of injury. Hsp70... -
Exendin-4 improves resistance to Listeria monocytogenes infection in diabetic db/db mice
Hsien Yueh Liu, Chih-Yao Chung, Wen-Chin Yang, Chih-Lung Liang, Chi-Young Wang, Chih-Yu Chang, Cicero Lee-Tian Chang* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 5 245-252 (8 pages)
The incidence of diabetes mellitus is increasing among companion animals. This disease has similar characteristics in both humans and animals. Diabetes is frequently identified as an independent risk factor for infections associated with increased mortality. In the present study, homozygous diabetic (db/db) mice were infected with Listeria (L.) monocytogenes and then treated with the anti-diabetic drug exendin-4, a glucagon-like peptide 1 analogue. In aged db/db mice, decreased CD11b macrophage... -
Evidence for estrogen receptor expression during medullary bone formation and resorption in estrogen―treated male Japanese quails (Coturnix coturnix japonica)
Shinji Hiyama*, Toshie Sugiyama, Seiji Kusuhara, Takashi Uchida 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 3호 2 223-227 (5 pages)
The temporal expression of estrogen receptor (ER)―a and ER―b mRNA was examined in male Japanese quails. Femurs of quails receiving 17b―estradiol underwent RT―PCR and histochemical analysis 1 to 15 days after treatment. Untreated quails were used as controls (day 0). Between days 0 and 5, cells lining the bone endosteal surface differentiated into osteoblasts, which in turn formed medullary bone. Expression of ER―a was already observed on day 0 and increased slightly during bone formation... -
Evaluation of the effect of a 0.0584% hydrocortisone aceponate spray on clinical signs and skin barrier function in dogs with atopic dermatitis
Eui-Hwa Nam, Seol-Hee Park, Ji-Young Jung, Seung-Hee Han, Hwa-Young Youn, Jun-Seok Chae, Cheol-Yong Hwang* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 10 187-191 (5 pages)
The purpose of this study was to evaluate the effects of a topical spray containing 0.0584% hydrocortisone aceponate (HCA) on canine atopic dermatitis (CAD) and to evaluate the skin barrier function during the treatment of CAD. Twenty-one dogs that fulfilled the diagnostic criteria for CAD were included in this study. The HCA spray was applied once a day to the lesions of all dogs for 7 or 14 days. Clinical assessment was performed before (day 0) and after treatment (day 14), and clinical... -
Sequence variations of the bovine prion protein gene (PRNP) in native Korean Hanwoo cattle
Sangho Choi, Hee-Jong Woo*, Jeongmin Lee* 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 3 127-137 (11 pages)
region of PRNP, determined their frequency, and evaluated their significance. We identified four synonymous polymorphisms and two non-synonymous mutations in PRNP, but found no novel polymorphisms. The sequence and number of octapeptide repeats were completely conserved, and the haplotype frequency of the coding region was similar to that of other B. taurus strains. When we examined the 23-bp and 12-bp insertion/deletion (indel) polymorphisms in the non-coding region of PRNP, Hanwoo cattle had a... -
Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea
Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 7 43-48 (6 pages)
Korea were isolated and propagated in murine neuroblastoma (NG108-15) cells with titers ranging from 10 to 10 TCID50/mL. Although the RIDA findings did not completely coincide with results obtained from FAT, VI, and RT-PCR, RIDA appears to be a fast and reliable assay that can be used to analyze brain samples. In summary, the results from our study showed that VI, RT- PCR, and RIDA can be used as supplementary diagnostic tools for detecting rabies viruses in both laboratory and field settings. -
The effect of conglutinin on production of reactive oxygen species in bovine granulocytes
Marta Dec*, Andrzej Wernicki, Andrzej Puchalski, Renata Urban-Chmiel, Adam Wa?ko 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 5 33-38 (6 pages)
immune defense mechanisms, showing antiviral and antibacterial activities when tested in vivo and in vitro. The present study evaluated the effect of conglutinin on the respiratory bursts in bovine peripheral phagocytes. Using nitroblue tetrazolium and hydrogen peroxide assays, we showed that sugar ligand- bound conglutinin stimulated the production of superoxide and H2O2 in granulocytes whereas the non-sugar-bound form of conglutinin inhibited these processes. These results indicate that both...


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