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전체 선택해제
총 469건 검색
본문검색 결과만 보기
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활성화된 Sepharose Gels에 공유결합으로 고정화된 Urokinase를 이용한 융합단백질 절단반응
서창우, 강관엽, 이효실, 안상점, 이은규 한국생물공학회 한국생물공학회지 7 Pages
한국생물공학회 한국생물공학회지 2000, Vol.15 No.1 42-48 (7 pages)
본 연구에서는 고정화 UK를 이용한 융합단백질의 절단방응에 대해 UK의 고정화, 고정화 UK의 특성과 절단방응, 절단반응 후의 분리정제 그리고 고정화 UK으 재생에 대해 실험하였다. 고정화 수율은 99% 이상이였고 고정화 후의 효소활성은 80%를 유지하였다. 융합단백질 전단반응에서 액상 UK와 고정화 UK를 이용한 회분식 반응 모두 약 70%의 절단반응을 얻었고, 특히 고정화 UK의 사용시 부반응이 매우 낮은 이점이 있었다. 컬럼식 절단반응에서는 기절의 주입속도에 따라 절단수율은 크게 변화하였다. 최적의 유속은 50%의 절단수율을... -
Amyloid β-InducedImpairment of autophagolysosome maturation play an important role in neuronalcell death through Accumulation of Damaged Mitochondria
Dah Ihm Kim, Jung Min Ryu, Ki Hoon Lee, Jeong Yeon Kim, Gee Euhn Choi, Ing Ing Chai, Ho Jae Han 대한수의학회 대한수의학회 학술대회발표집 1 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 269 482-482 (1 pages)
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Effect of serum-derived albumin scaffold and canine adipose tissue-derived mesenchymal stem cells on osteogenesis in canine segmental bone defect model
Daeyoung Yoon, Byung-Jae Kang, Yongsun Kim, Seung Hoon Lee, Daeun Rhew, Wan Hee Kim, Oh-Kyeong Kweon 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 2 397-404 (8 pages)
Composite biological and synthetic grafts with progenitor cells offer an alternative approach to auto- or allografts for fracture repair. This study was conducted to evaluate osteogenesis of autologous serum-derived albumin (ASA) scaffolds seeded with canine adipose tissue-derived mesenchymal stem cells (Ad-MSCs) in a canine segmental bone defect model. ASA scaffold was prepared with canine serum using cross-linking and freeze-drying procedures. Beta-tricalcium phosphate (β-TCP) was mixed at... -
Comparable bone healing capacity of different bone graft matrices in a rabbit segmental defect model
Jong Min Kim, Myoung Hwan Kim, Seong Soo Kang, Gonhyung Kim, Seok Hwa Choi* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 16 289-295 (7 pages)
We compared the bone healing capacity of three different demineralized bone matrix (DBM) products applied using different carrier molecules (hyaluronic acid [HA] vs. carboxymethylcellulose [CMC]) or bone compositions (cortical bone vs. cortical bone and cancellous bone) in a rabbit segmental defect model. Overall, 15-mm segmental defects in the left and right radiuses were created in 36 New Zealand White rabbits and filled with HA-based demineralized cortical bone matrix (DBX), CMC-based... -
Isolation and characterization of canine umbilical cord blood-derived mesenchymal stem cells
Min-Soo Seo, Yun-Hyeok Jeong, Jeung-Ran Park, Sang-Bum Park, Kyoung-Hwan Rho, Hyung-Sik Kim, Kyung-Rok Yu, Seung-Hee Lee, Ji-Won 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 3호 2 181-187 (7 pages)
the neuronal markers glial fibrillary acidic protein (GFAP), neuronal class III b tubulin (Tuj-1), neurofilament M (NF160) in the basal culture media. After neuronal differentiation, the cMSCs expressed the neuronal markers Nestin, GFAP, Tuj-1, microtubule- associated protein 2, NF160. In the osteogenic & chondrogenic differentiation studies, cMSCs were stained with alizarin red and toluidine blue staining, respectively. With osteogenic differentiation, the cMSCs presented osteoblastic... -
Lysophosphatidic acid enhances breast cancer cells-mediated osteoclastogenesis
Ju-Suk Nam, Ashish Ranjan Sharma, Lich Thi Nguyen, Supriya Jagga, Yeon-Hee Lee, Garima Sharma, Sang-Soo Lee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2018, Vol.22 No.5 4 503-511 (9 pages)
Lysophosphatidic acid (LPA) is known to play a critical role in breast cancer metastasis to bone. In this study, we tried to investigate any role of LPA in the regulation of osteoclastogenic cytokines from breast cancer cells and the possibility of these secretory factors in affecting osteoclastogenesis. Effect of secreted cytokines on osteoclastogenesis was analyzed by treating conditioned media from LPA-stimulated breast cancer cells to differentiating osteoclasts. Result demonstrated that...
