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Effects of High Glucose on Na,K-ATPase and Na/glucose Cotransporter Activity in Primary Rabbit Kidney Proximal Tubule Cells
Han, Ho-Jae 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 7 69-80 (12 pages)
Renal proximal tubular hypertrophy and hyperfunction are known to be early manifestations of experimental and human diabetes. As the hypertrophy and hyperfunction have been suggested to be central components in the progression to renal failure, an understanding of their underlying causes is potentially important for the development of therapy. A primary rabbit kidney proximal tubule cell culture system was utilized to evaluate the possibility that the renal proximal tubular hypertrophy and... -
The Action of Ginkgo Bibloba Extract in the Isolated Rabbit Corpus Cavernosum
Chung, Woo-Sik, Choi, Young-Deuk, Park, Young-Yo, Hah, Jong-Sik 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 6 61-68 (8 pages)
The extract of Ginkgo bibloba (EGb) is a complex mixture of natural products from the Ginkgo leaves and clinically used for the treatment of cerebral and peripheral circulatory disturbances due to its combined activity of several vasoactive principles. In this study we investigated the action of EGb and its mechanism in isolated rabbit corporal smooth muscle to evaluate the possibility of using this material as a pharmacoerecting agent. Strips of rabbit corpus cavernosum were mounted in organ... -
Effect of Cyclic Nucleotides on Phorbol Ester-Induced Contraction in Rabbit Carotid Artery
Jung, Dong-Keun, Woo, Jae-Suk, Jung, Jin-Sup, Kim, Yong-Keun, Lee, Sang-Ho 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 4 39-50 (12 pages)
This study was designed to clarify the action of cyclic nucleotides, cyclic AMP and cyclic GMP, on phorbol 12,13-dibutyrate (PDBu)-induced contraction in rings isolated from rabbit carotid artery. Arterial rings, 2 mm in width, were myographied isometrically in an isolated organ bath. PDBu produced slowly developing, sustained contraction in rabbit carotid artery, in a dose dependent manner, which was independent of extracellular Ca2+ PDBu-induced contraction was relaxed by staurosporine, which... -
Inhibitory Effects of Verapamil and TMB-8 on Tonic Contraction Are Accompanied by Inhibition of Phospholipase C Activity in Intact Gastric Smooth Muscle Cells
Sim, Sang-Soo, Yoon, Shin-Hee, Hahn, Sang-June, Rhie, Duck-Joo, Jo, Yang-Hyeok, Kim, Myung-Suk 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1995, 제 29권 제 1호 3 29-38 (10 pages)
Gastric smooth muscle of guinea pigs was used to investigate whether the inhibitory effect of calcium antagonists on tonic contraction was accompanied by inhibition of phospholipase C activity. Tonic contraction and [3H] inositol phosphate (IP) formation in response to acetylcholine were measured after pretreatment with verapamil, nifedipine, 8-(N,N-diethylamino)octyl 3,4,5-trimethoxy-benzoate (TMB-8) or EGTA. Verapamil (10 μM), TMB-8 (10 μM) or EGTA (2 mM) significantly inhibited... -
Transformation of Rabbit Proximal Tubule Cells by Strontium Phosphate Transfection with a Plasmid Containing SV4O Early Region Genes
Han, Ho-Jae, Taub, Mary L., 대한생리학회 대한생리학회지 8 Pages
대한생리학회 대한생리학회지 1994, 제 28권 제 2호 14 233-240 (8 pages)
In this study, it was investigated whether immortalized proximal tubule cells transformed with pRSVT could survive through the numerous passages. Results were as follows: 1. The cells transfected with pRSVT formed rapidly growing, multilayered colonies within 2 weeks in a hormone defined medium. Domes were also observed in some of the cultures. 2. r-glutamyl transpeptidase activity was equivalent to that observed in primary renal proximal tubule cell cultures. 3. Transformed cells with pRSVT... -
Immunocytochemical Study on the Translocation Mechanism of Glucose Transporters by Insulin
Hah, Jong-Sik, Kim, Ku-Ja 대한생리학회 대한생리학회지 16 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 2호 2 123-138 (16 pages)
The mechanism of insulin action to increase glucose transport is attributed to glucose transporter translocation from intracellular storage pools to the plasma membrane in insulin-sensitive cells. The present study was designed to visualize the redistribution of the glucose transporter by means of an immunogold labelling method. Our data clearly show that glucose transporter molecules were visible by this method. According to the method this distribution of glucose transporters between cell... -
Inhibition of Dicarboxylate Transport by p-chloromercuribenzoic Acid (PCMB) in Plasma Membrane Vesicles of Rabbit Proximal Tubule
Kim, Yong-Keun, Kim, Tae-In, Jung, Jin-Sup, Lee, Sang-Ho 대한생리학회 대한생리학회지 10 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 2호 8 179-188 (10 pages)
Effect of a sulfhydryl reagent, p-chloromercuribenzoic acid (PCMB), on the transport of succinate was studied in brush border (BBMV) and basolateral (BLMV) membrane vesicles isolated from rabbit renal cortex. PCMB induced an irreversible inhibition of the Na+-dependent succinate uptake in a dose-dependent manner with IC50 of 55 and 65 μM in BBMV and BLMV, respectively. The inhibitory effect of PCMB was prevented by a pretreatment of vesicles with dithiothreitol. PCMB did not increase Na+... -
Central Projections of Sensory Information Produced by Topical Application of Capsaicin to the Tongue in the Cat
Hong, Seung-Kil, Nahm, Sook-Hyun, Han, Hee-Chul, Suh, Young-Suk, Won, Ran, Lee, Bae-Hwan 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1991, 제 25권 제 1호 9 87-100 (14 pages)
In order to elucidate whether capsaicin applied topically acts as a pain-producing subastance or as a tastant, neuronal activities of the chorda tympani nerve(CN), lingual nerve(LN), solitary tract nucleus(STN), and trigeminal nucleus(TGN) were recorded while thermal and taste stimuli, and capsaicin were being applied topically, and algesics intra-arterially to the tongue of cats anesthetized with α-chloralsoe. In addition, the STN neurons were examined after wheat germ agglutinin-horseradish... -
고양이 회장 종주근에서 Na-Ca 교환 기전의 특성에 관한 연구
우재석(Woo, Jae-Suk), 서덕준(Suh, Duk-Joon), 김용근(Kim, Yong-Keun), 이상호(Lee, Sang-Ho) 대한생리학회 대한생리학회지 16 Pages
대한생리학회 대한생리학회지 1989, 제 23권 제 2호 2 237-252 (16 pages)
고양이 회장 종주근에서 세포막 소포를 분리하여 Na+의 농도 경사에 의존하여 일어나는 Ca2+ 이동의 특성에 대하여 연구하였다. 막소포 내부에서 외부로 향하는 Na+의 농도 경사 존재시 Ca2+의 축적이 현저히 증가하여 Na+ 의존성 Ca2+ 축적을 보였으며, 이는 외부용액에 Na+ ionophore인 monensin을 처리시 소실되었다. 한편 이러한 Ca2+ 축적의 증가 작용은 Na+에 특이적이었으며 K+, Li+, Rb+, Cs+ 및 choline이온은 Na+의 작용을 대치하지 못하였다. Ba2+, Sr2+, Mn2+ 및 Cd2+ 등의 2가 양이온들은 0.5 mM의 농도에서 Na+ 의존성 Ca2+... -
가토 근위세뇨관 Basolateral Membrane Vesicle에서 Succinate 이동 특성
김용근(Kim, Yong-Keun), 배혜란(Bae, Hae-Rahn), 임병용(Rhim, Byung-Yong) 대한생리학회 대한생리학회지 12 Pages
대한생리학회 대한생리학회지 1988, 제 22권 제 2호 13 307-318 (12 pages)
가토 신장 피질에서 Percoll density gradient방법으로 분리한 basolateral membrane vesicle (BLMV)에서 rapid filtration technique을 이용하여 succinate의 이동 특성을 관찰하였다. Na+은 succinate의 이동을 증가시켜 overshoot 현상을 보였으며 이러한 효과는 K+, Li+, Rb+, choline 과 같은 다른 양이온들에 의해 나타나지 않았다. Na+농도변화에 따른 succinate의 이동율은 sigmoid모양을 보였고, Na+에 대한 Hill coefficient는 2.0이었다. soccinate의 이동은 vesicle 내부가 음전압일 때 더욱 증가되었다. BLMV에서...
