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Development of a Daily Epidemiological Model of Rice Blast Tailored for Seasonal Disease Early Warning in South Korea
Kwang-Hyung Kim, Imgook Jung 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 3 406-417 (12 pages)
into a realistic and measurable disease value through statistical analyses with 13 rice blast incidence datasets, and subsequently validated using the data from another rice blast experiment conducted in Icheon, South Korea, from 1974 to 2000. The sensitivity of the model to air temperature, relative humidity, and precipitation input variables was examined, and the relative humidity resulted in the most sensitive response from the model. Overall, our results indicate that the EPIRICE Daily Risk... -
Investigating the Induced Systemic Resistance Mechanism of 2,4-Diacetylphloroglucinol (DAPG) using DAPG Hydrolase-Transgenic Arabidopsis
Dae-Han Chae, Da-Ran Kim, Mi Sun Cheong, Yong Bok Lee, Youn-Sig Kwak 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 6 255-266 (12 pages)
Plant immune responses can be triggered by chemicals, microbes, pathogens, insects, or abiotic stresses. In particular, induced systemic resistance (ISR) refers to the activation of the immune system due to a plant’s in- teraction with beneficial microorganisms. The phenolic compound, 2,4-diacetylphloroglucinol (DAPG), which is produced by beneficial Pseudomonas spp., acts as an ISR elicitor, yet DAPG’s mechanism in ISR remains unclear. In this study, transgenic Arabidopsis thaliana plants... -
Bacteriophage Usage for Bacterial Disease Management and Diagnosis in Plants
Nguyen Trung Vu, Chang-Sik Oh 한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 3호 2 204-217 (14 pages)
In nature, plants are always under the threat of pests and diseases. Pathogenic bacteria are one of the ma- jor pathogen types to cause diseases in diverse plants, resulting in negative effects on plant growth and crop yield. Chemical bactericides and antibiotics have been used as major approaches for controlling bacterial plant diseases in the field or greenhouse. However, the appearance of resistant bacteria to common antibiot- ics and bactericides as well as their potential negative effects... -
Rapid Detection of Lily mottle virus and Arabis mosaic virus Infecting Lily (Lilium spp.) Using Reverse Transcription Loop-Mediated Isothermal Amplification
Yubao Zhang, Yajun Wang, Zhongkui Xie, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 2호 6 170-178 (9 pages)
cross-reactivity from other lily viruses. Optimal conditions for LAMP reactions were 65°C and 60°C for 60 min for LMoV and ArMV, respectively. Detection sensitivity for both RT-LAMP assays was a hundredfold greater than that of our comparative RT-polymerase chain reaction as- says. We have also found this relatively rapid, target specific and sensitive method can also be used for samples collected in the field and may be especially useful in regions with limited or no laboratory facilities. -
Rapid and Sensitive Detection of Lettuce Necrotic Yellows Virus and Cucumber Mosaic Virus Infecting Lettuce (Lactuca sativa L.) by Reverse Transcription Loop-Mediated Isothermal Amplification
Yubao Zhang, Zhongkui Xie, John D Fletcher, Yajun Wang, Ruoyu Wang, Zhihong Guo, Yuhui He 한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 7 76-86 (11 pages)
Optimum LAMP reactions were conducted in betaine-free media with 6 mM Mg2+ at 65°C for LNYV and 60°C for 60 min for CMV, respectively. The detection limit was 3.5 pg/ml and 20 fg/ml using RT-LAMP for LNYV and CMV plasmids, respectively. Detection sensitivity for both RT-LAMP assays was greater by a factor of 100 compared to the conventional reverse transcription polymerase chain reaction assays. This rapid, specific, and sensitive technique should be more widely applied due to its low cost and... -
Application of Numerical Weather Prediction Data to Estimate Infection Risk of Bacterial Grain Rot of Rice in Korea
Hyo-suk Kim, Ki Seok Do, Joo Hyeon Park, Wee Soo Kang, Yong Hwan Lee, Eun Woo Park 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 1호 5 54-66 (13 pages)
on bacterial grain rot (BGR) of rice was examined as compared with the model output based on the automated weather stations (AWS)-observed weather data. We analyzed performance of BGRcast based on the UM-predicted and the AWS-observed daily minimum temperature and average relative humidity in 2014 and 2015 from 29 locations representing major rice growing areas in Korea using regression analysis and two-way contingency table analysis. Temporal changes in weather conduciveness at two locations in... -
IsIsolation and Characterization of Airborne Mushroom Damaging Trichoderma spp. from Indoor Air of Cultivation Houses Used for Oak Wood Mushroom Production Using Sawdust Mediaolation and Characterization of Airborne Mushroom Damaging Trichoderma spp. from
Jun Young Kim, Hyuk Woo Kwon, Dong Hyeung Lee, Han Kyu Ko, Seong Hwan Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 13 674-683 (10 pages)
Some species of the Trichoderma genus are reported as the major problem in oak wood mushroom production in Korea. In spite of economic loss by the fungi, scientific information on airborne Trichoderma species is not much available. To generate information for disease management development we analyzed airborne Trichoderma. A total of 1,063 fungal isolates were purely obtained from indoor air sampling of cultivation houses used for oak wood mushroom using sawdust media. Among the obtained... -
Identification of Heterodera glycines (Tylenchida; Heteroderidae) Using qPCR
Hyoung-Rai Ko, Heonil Kang, Eun-Hyoung Park, Eun-Hwa Kim, Jae-Kook Lee 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 11 654-661 (8 pages)
from a single cyst and single second-stage juvenile (J2). The specificity was confirmed by the absence of SYBR fluorescent signals of three other Heterodera species. A serial dilution of DNA extracted from a single cyst was obtained for the sensitivity test. The result showed that the standard curve of the test had a highly significant linearity between DNA concentration and Ct value (R2 = 0.996, slope = −3.49) and that the detection limit concentration of DNA of the primer set was 10 pg... -
Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches
Weilan Li, Seung-Yeol Lee, Chang-Gi Back, Leonid N. Ten, Hee-Young Jung 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 9 635-643 (9 pages)
To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA... -
Optimization of Polyethylene Glycol-Mediated Transformation of the Pepper Anthracnose Pathogen Colletotrichum scovillei to Develop an Applied Genomics Approach
Jong-Hwan Shin, Joon-Hee Han, Hyun-Hoo Park, Teng Fu, Kyoung Su Kim 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 4 575-584 (10 pages)
Colletotrichum acutatum is a species complex responsible for anthracnose disease in a wide range of host plants. Strain C. acutatum KC05, which was previously isolated from an infected pepper in Gangwon Province of South Korea, was reidentified as C. scovillei using combined sequence analyses of multiple genes. As a prerequisite for understanding the pathogenic development of the pepper anthracnose pathogen, we optimized the transformation system of C. scovillei KC05. Protoplast generation from...


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