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461.

Induction of antibody and interferon-γ production in mice immunized with virus-like particles of swine hepatitis E virus

Young-Jo Song, Woo-Jung Park, Seul-Kee Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Kun-Ho Seo, Young-Sun 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 15 575-578 (4 pages)

Young-Jo Song, Woo-Jung Park, Seul-Kee Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Sang-Won Lee, Kun-Ho Seo, Young-Sun 저
...
- 1,500원
Virus-like particles (VLPs) composed of the truncated capsid protein of swine hepatitis E virus (HEV) were developed and immune responses of mice immunized with the VLPs were evaluated. IgG titers specific for the capsid protein of swine HEV were significantly higher for all groups of mice immunized with the VLPs than those of the negative control mice. Splenocytes from mice immunized with the VLPs also produced significantly greater quantities of interferon (IFN)-γ than interleukin (IL)-4 and...
462.

Epidemiology of brucellosis among cattle in Korea from 2001 to 2011

Hachung Yoon, , Oun-Kyong Moon, Soo-Han Lee, Won-Chang Lee, Moon Her, Wooseog Jeong, Suk-Chan Jung, Do-Soon Kim 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 11 537-543 (7 pages)

Hachung Yoon, , Oun-Kyong Moon, Soo-Han Lee, Won-Chang Lee, Moon Her, Wooseog Jeong, Suk-Chan Jung, Do-Soon Kim 저
...
- 3,000원
In the present study, the outbreak patterns of bovine brucellosis in Korea from 2000 to 2011 were analyzed to understand the epidemiological evolution of this disease in the country. A total of 85,521 brucella reactor animals were identified during 14,215 outbreaks over the 12-year study period. The number of bovine brucellosis cases increased after 2003 and peaked in 2006 before decreasing thereafter. The majority of the bovine brucellosis cases were Korean native cattle, Han Woo. The numbers...
463.

Enhancing effects of serum-rich and cytokine-supplemented culture conditions on developing blastocysts and deriving porcine parthenogenetic embryonic stem cells

Soo-Kyung Jung, Hyun-Jung Kim, Chan-Lan Kim, Joo-Hyeong Lee, Jin-Young You, Eun-Song Lee, Jeong-Mook Lim, Seon Jong Yun, Jae-You 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 9 519-528 (10 pages)

Soo-Kyung Jung, Hyun-Jung Kim, Chan-Lan Kim, Joo-Hyeong Lee, Jin-Young You, Eun-Song Lee, Jeong-Mook Lim, Seon Jong Yun, Jae-You 저
...
- 3,000원
The present study was conducted to develop an effective method for establishment of porcine parthenogenetic embryonic stem cells (ppESCs) from parthenogenetically activated oocyte-derived blastocysts. The addition of 10% fetal bovine serum (FBS) to the medium on the 3rd day of oocyte culturing improved the development of blastocysts, attachment of inner cell masses (ICMs) onto feeder cells, and formation of primitive ppESC colonies. ICM attachment was further enhanced by basic fibroblast growth...
464.

Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development

Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 6 503-509 (7 pages)

Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 저
...
- 3,000원
Simple methods for measuring the levels of serum antibody against canine distemper virus (CDV) would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific for CDV, we expressed hydrophilic extra-viral domain (HEVD) protein of the A75/17-CDV H gene in a pET 28a plasmid-based Escherichia (E.) coli vector system. Expression was confirmed by dot and Western blotting. We proposed that detection of E. coli-expressed H protein might be...
465.

N-acetylcysteine protects against cadmium-induced oxidative stress in rat hepatocytes

Jicang Wang, Huali Zhu, Xuezhong Liu, Zongping Liu, 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 4 485-493 (9 pages)

Jicang Wang, Huali Zhu, Xuezhong Liu, Zongping Liu, 저
...
- 3,000원
Cadmium (Cd) is a well-known hepatotoxic environmental pollutant. We used rat hepatocytes as a model to study oxidative damage induced by Cd, effects on the antioxidant systems, and the role of N-acetylcysteine (NAC) in protecting cells against Cd toxicity. Hepatocytes were incubated for 12 and 24 h with Cd (2.5,  5, 10 μM). Results showed that Cd can induce cytotoxicity: 10 μM resulted in 36.2% mortality after 12 h and 47.8% after 24 h. Lactate dehydrogenase, aspartate...
466.

Stanniocalcin-1 protects bovine intestinal epithelial cells from oxidative stress-induced damage

Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 3 475-483 (9 pages)

Li-ming Wu, Rui Guo, Lin Hui, Yong-gang Ye, Jing-mei Xiang, Chun-yun Wan, Miao Zou, Rui Ma, Xiao-zhuan Sun, Shi-jin Yang, Ding-z 저
...
- 3,000원
for 48 h to over-express STC-1. Acridine orange/ ethidium bromide (AO/EB) double staining and trypan blue exclusion assays were then performed to measure cell viability and apoptosis of the cells, respectively. The expression of STC-1 and apoptosis-related proteins in the cells was monitored by real-time PCR and Western blotting. The results indicated that both STC-1 mRNA and protein expression levels positively correlated with the duration of H2O2 treatment. H2O2 damaged the bovine IECs in a...
467.

Effects of progestagen exposure duration on estrus synchronization and conception rates of crossbreed ewes undergoing fixed time artificial insemination

Wanessa Blaschi, Paula A. Lunardelli, Luciana S.R. Marinho, Marilu C. Max, Gustavo M.G. Santos, Katia C. Silva-Santos, Fabiana A 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 13 433-437 (5 pages)

Wanessa Blaschi, Paula A. Lunardelli, Luciana S.R. Marinho, Marilu C. Max, Gustavo M.G. Santos, Katia C. Silva-Santos, Fabiana A 저
...
- 1,500원
Synchronization of estrus and ovulation are of paramount importance in modern livestock improvement programs. These methods are critical for assisted reproduction technologies, including artificial insemination and embryo transfer, that can increase productivity. In the current study, subcutaneous implants containing norgestomet were placed for long (14 days), medium (9 days), and short (5 days) periods of time in 70 crossbred ewes undergoing fixed-time artificial insemination. The resulting...
468.

Use of a domestic Korean black goat (Capra hircus coreanae) with its chest crayon-harnessed in detecting estrus of Himalayan tahrs (Hemitragus jemlahicus)

Hwanyul Yong, Eunsong Lee* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 12 427-432 (6 pages)

Hwanyul Yong, Eunsong Lee* 저
...
- 3,000원
h later. Two females treated with sponges containing 60 or 45 mg of FGA had estrogen levels of 8.7 and 11.1 pg/mL, respectively. No red marks were found on the backs of these two tahrs. The remaining females had higher levels of estradiol, and the red crayon marks were clearly shown. The cervical folds of these tahrs were readily penetrated and the insemination gun was smoothly inserted into the uterine body. In conclusion, a Korean domestic goat with its chest crayon-harnessed was successfully...
469.

Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs

Min-Hee Kang, Min-Joo Chae, Jang-Won Yoon, Seung-Gon Kim, So-Young Lee, Jong-Hyun Yoo, Hee-Myung Park* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 9 409-415 (7 pages)

Min-Hee Kang, Min-Joo Chae, Jang-Won Yoon, Seung-Gon Kim, So-Young Lee, Jong-Hyun Yoo, Hee-Myung Park* 저
...
- 3,000원
The prevalence, virulence potential, and antibiotic resistance of ophthalmic Staphylococcus pseudintermedius (SP) isolated from dogs were examined. Sixty-seven Staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the Staphylococcus intermedius group (SIG) nuclease gene (SInuc), exfoliative toxin gene for SIG (siet), and antibiotic resistance genes (blaZ and mecA). PCR-restriction fragment length polymorphism analysis of the pta gene was also...
470.

Molecular characterization of duck enteritis virus CHv strain UL49.5 protein and its colocalization with glycoprotein M

Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 7 389-398 (10 pages)

Meng Lin, Renyong Jia*, Mingshu Wang, Xinghong Gao, Dekang Zhu, Shun Chen, Mafeng Liu, Zhongqiong Yin, Yin Wang, Xiaoyue Chen, A 저
...
- 3,000원
The UL49.5 gene of most herpesviruses is conserved and encodes glycoprotein N. However, the UL49.5 protein of duck enteritis virus (DEV) (pUL49.5) has not been reported. In the current study, the DEV pUL49.5 gene was first subjected to molecular characterization. To verify the predicted intracellular localization of gene expression, the recombinant plasmid pEGFP-C1/pUL49.5 was constructed and used to transfect duck embryo fibroblasts. Next, the recombinant plasmid pDsRed1-N1/ glycoprotein M (gM)...

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