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Enhancement of protective immune responses by oral vaccination with Saccharomyces cerevisiae expressing recombinant Actinobacillus pleuropneumoniae ApxIA or ApxIIA in mice
Sung Jae Shin, Seung Won Shin, Mi Lan Kang, Deog Yong Lee, Moon-Sik Yang, Yong-Suk Jang, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 11 383-392 (10 pages)
We previously induced protective immune response by oral immunization with yeast expressing the ApxIIA antigen. The ApxI antigen is also an important factor in the protection against Actinobacillus pleuropneumoniae serotype 5 infection; therefore, the protective immunity in mice following oral immunization with Saccharomyces cerevisiae expressing either ApxIA (group C) or ApxIIA (group D) alone or both (group E) was compared with that in two control groups (group A and B). The immunogenicity of... -
Time of initial detection of fetal and extra-fetal structures by ultrasonographic examination in Miniature Schnauzer bitches
Bang-Sil Kim, Chang-Ho Son 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 3호 14 289-293 (5 pages)
Serial ultrasonographic examinations were performed daily on 9 Miniature Schnauzer bitches from the 15th day of gestation until parturition to determine the time the gestational structures were first detected. The gestational age was timed from the day of ovulation (day 0), which was estimated to occur when the plasma progesterone concentration was >4.0 ng/ml. The gestational length in 9 Miniature Schnauzer bitches was found to be 63.0 ± 1.7 (range 61-65) days. The initial detection of the... -
Diagnosis of Helicobacter spp. infection in canine stomach
W. Prachasilpchai, S. Nuanualsuwan, T. Chatsuwan, S. Techangamsuwan, S. Wangnaitham, A. Sailasuta 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 6 139-145 (7 pages)
A total of 75 biopsied samples of cardia, fundus, body, and pyloric antrum from necropsied dogs that were submitted to the Department of Pathology, Faculty of Veterinary Science, Chulalongkorn University from April 2003 to June 2004 were investigated. The objectives of this study were to determine the prevalence of Helicobacter spp. in canine stomach by polymerase chain reaction (PCR) in comparison to histochemistry versus immunohistochemistry (IHC), and to correlate these diagnostic methods... -
Establishment of a canine spinal cord injury model induced by epidural balloon compression
Ji-Hey Lim, Chang-Su Jung, Ye-Eun Byeon, Wan Hee Kim, Jung-Hee Yoon, Kyung-Sun Kang, Oh-kyeong Kweon 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 13 89-94 (6 pages)
A model that provides reproducible, submaximal yet sufficient spinal cord injury is needed to allow experiments leading to development of therapeutic techniques and prediction of clinical outcome to be conducted. This study describes an experimental model for spinal cord injury that uses three different volumes of balloon inflation and durations of compression to create a controlled gradation outcome in adult dogs. Twenty-seven mongrel dogs were used for this study. A 3-french embolectomy... -
Birth of puppies after intrauterine and intratubal insemination with frozen-thawed canine semen
Hye Jin Kim, Hyun Ju Oh, Goo Jang, Min Kyu Kim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 11 75-80 (6 pages)
The present study was performed to assess the fertility of frozen-thawed dog semen prepared by freezing with 6% glycerol and thawing at 70ºC for 8 sec, and to evaluate the least number of post-thaw spermatozoa necessary to achieve pregnancy by intrauterine or intratubal artificial insemination. It was found that the pregnancy rate of intrauterine artificial insemination was 100% using 6% glycerol buffer and thawing at 70ºC for 8 sec with 5 × 107 spermatozoa. Even though the pregnancy rate... -
Estrogen receptor independent neurotoxic mechanism of bisphenol A, an environmental estrogen
Yoot Mo Lee, Min Jae Seong, Jae Woong Lee, Yong Kyung Lee, Tae Myoung Kim, Sang-Yoon Nam, Dae Joong Kim, Young Won Yun, Tae Seon 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 5 27-38 (12 pages)
Bisphenol A (BPA), a ubiquitous environmental contaminant, has been shown to cause developmental toxicity and carcinogenic effects. BPA may have physiological activity through estrogen receptor (ER) -α and -β, which are expressed in the central nervous system. We previously found that exposure of BPA to immature mice resulted in behavioral alternation, suggesting that overexposure of BPA could be neurotoxic. In this study, we further investigated the molecular neurotoxic mechanisms of BPA. BPA... -
Organotypic slice culture of the hypothalamic paraventricular nucleus of rat
Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 3 15-20 (6 pages)
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and... -
Immunohistochemical localization of calcium binding proteins and some neurotransmitters in myenteric plexus of goat stomach
Heungshik S. Lee, Young Sam Nam 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 2 315-319 (5 pages)
To understand the neurochemical properties of the gastric myenteric plexus of ruminants, the expression patterns of calbindin D-28k (CB), calretinin (CR), substance P (SP) and calcitonin gene-related peptide (CGRP) were explored in the Korean native goat. In gastric myenteric plexus, CB and SP immunoreactivity were observed in round- or ovalshaped neurons. CR and CGRP immunoreactivity were detected only in the nerve fibers. This immunohistochemical localization of CB, CR, CGRP and SP in the... -
Genotoxicity and toxicological effects of acrylamide on reproductive system in male rats
Hye-Jin Yang, Sang-Hyun Lee, Yong Jin, Jin-Hyang Choi, Chang-Hoon Han, Mun-Han Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 2호 3 103-109 (7 pages)
The toxicity of acrylamide was evaluated through mutagenicity of Salmonella, chromosome aberration of Chinese hamster lung fibroblasts, micronucleus formation in mice and reproductive toxicity in rats. Based on Ames test, acrylamide showed mutagenic potency for strains TA98 and TA100. Moreover, both chromosomal aberration assay and micronucleus assay indicated that acrylamide might have genotoxic potency; the chromosomal aberration frequencies were observed to be proportional to acrylamide... -
Distribution of trkA in cerebral cortex and diencephalon of the mongolian gerbil after birth
Il-Kwon Park, Xilin Hou, Kyung-Youl Lee, O-Sung Park, Kang-Yi Lee, Min-Young Kim, Tae-Sun Min, Geun-Jwa Lee, Won-Sik Kim, Moo-Kan 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 4 303-307 (5 pages)
TrkA is essential components of the high-affinity NGF receptor necessary to mediate biological effects of the neurotrophins NGF. Here we report on the expression of trkA in the cerebral cortex and diencephalon of mongolian gerbils during postnatal development. The expression of trkA was identified by immunohistochemical method. In parietal cortex and piriform cortex, higher levels of trkA-IR (immunoreactivity) were detected at 3 days postnatal (P3) and at P9. Although trkA was not expressed till...


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