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Differential Activation of Ras/Raf/MAPK Pathway between Heart and Cerebral Artery in Isoproterenol-induced Cardiac Hypertrophy
HyunjuKim, NariKim, HyunJoo, JaeBoumYoum, WonSunPark, MohamedWarda, SunghyunKang, VuThiThu, TranMinhKhoa, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.5 7 299-304 (6 pages)
Cardiac hypertrophy contributes an increased risk to major cerebrovascular events. However, the molecular mechanisms underlying cerebrovascular dysfunction during cardiac hypertrophy have not yet been characterized. In the present study, we examined the molecular mechanism of isoproterenol (ISO)-evoked activation of Ras/Raf/MAPK pathways as well as PKA activity in cerebral artery of rabbits, and we also studied whether the activations of these signaling pathways were altered in cerebral artery,... -
Morphine-induced Modulation of Nociceptive Spinal Dorsal Horn Neuronal Activities after Formalin-induced Inflammatory Pain
JooMinPark, KangWuLi, SungJunJung, JunKim, SangJeongKim, 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 2 77-86 (10 pages)
the naïve state, suggesting that basal GABAA tone increased after the formalin injection. Muscimol, GABAA agonist, reduced the neuronal responses before the treatment with formalin, but not after formalin treatment, again indicating an increase in the GABAergic basal tone after the formalin injection which saturated the neuronal responses to GABA agonist. Morphine-induced increase in the spinal nociceptive responses after formalin treatment was inhibited by co-application of muscimol. These... -
Effect of Defibrotide on Rat Reflux Esophagitis
HyoungKiKim, SooRanChoi, SangJinChoi, MyungSupChio, YongKyooShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.6 5 319-328 (10 pages)
This study was aimed at evaluating the effect of defibrotide on the development of the surgically induced reflux esophagitis, on gastric secretion, lipid peroxidation, polymorphonuclear leukocytes (PMNs) accumulation, polymorphonuclear leukocytes adherence, superoxide anion and hydrogen peroxide production in PMNs, scavenge of hydroxyl radical and hydrogen peroxide, cytokine (interleukin-1β, tumor necrosis factor-α) production in blood, and intracellular calcium mobilization in PMNs.... -
Influence of Cilnidipine on Catecholamine Release in the Perfused Rat Adrenal Medulla
Seong-ChangWoo, Young-JooBaek, Dong-YoonLim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.5 5 265-272 (8 pages)
dose of cilnidipine did not affect CA secretion by high K (5.6⁓102 M), higher dose of it reduced greatly CA secretion of high K. Cilnidipine itself did fail to affect basal catecholamine output. In the presence of cilnidipine (10μM), the CA secretory responses evoked by Bay-K-8644 (10μM), an activator of L-type Ca2 channels and cyclopiazonic acid (10μM), an inhibitor of cytoplasmic Ca2-ATPase were also inhibited. Moreover, ω-conotoxin GVIA (1μM), a... -
Effect of Mild Hypothermia on the Mitogen Activated Protein Kinases in Experimental Stroke
HyungSooHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.4 3 187-194 (8 pages)
p38 was observed in the vessels at 3 h, neuron-like cells at 6 and 12 h and glia-like cells at 12 h. Activation of JNK was not remarkable, and a few cells showed active JNK following ischemia. Phosphorylation of Elk-1, a transcription factor, was reduced by ischemic insult. Hypothermia attenuated the activation of ERK, p38 and JNK, and inhibited reduction of Elk-1. These data suggest that signals via different MAPK family members converge on the cell damage process and hypothermia protects the... -
Effect of Amrinone, a Selective Inhibitor of Phosphodiesterase III, on PMNs-induced
ByungKwonOh, HyoungKiKim, SooRanChoi, JinHoSong, EonSubPark, Byung 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.1 7 43-50 (8 pages)
reperfusion, significantly improved coronary flow, left ventricular developed pressure (P＜0.001), and the maximal rate of development of left ventricular developed pressure (P＜0.001), compared with ischemic/reperfused hearts perfused with PMNs in the absence of amrinone. In addition, amrinone significantly reduced myeloperoxidase activity by 50.8%, indicating decreased PMNs infiltration (p＜0.001). Superoxide radical and hydrogen peroxide production were also significantly reduced in fMLP- and... -
Cooperation of Gβ and Gαq Protein in Contractile Response of Cat Lower Esophageal Sphincter (LES)
UyDongSohnTaiSangLee 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.6 9 349-355 (7 pages)
dose dependent manner in LES and esophageal muscle cells obtained by enzymatic digestion with collagenase. PLC-β1 or PLC-β3 antibody incubation reduced contraction in response to ACh in LES but not in esophageal permeabilized cells, but PLC-γ1 antibody incubation did not have an inhibitory effect. The inhibition by PLC-β1 or PLC-β3 antibody on Ach-induced contraction was antibody concentration dependent. The combination with PLC-β1 and PLC-β3 antibody completely abolished the contraction,... -
Antagonists of NMDA Receptor, Calcium Channel and Protein Kinase C Potentiate Inhibitory Action of Morphine on Responses of Rat Dorsal Horn Neuron
HongKeeShin, YeonSukKim, JongHunJun, SeoEunLee, JaeHwaKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.5 1 251-254 (4 pages)
The present study was designed to examine whether the co-application of morphine with Ca2 channel antagonist (Mn2, verapamil), N-methyl-D-aspartate (NMDA) receptor antagonist (2-amino-5-phosphonopentanoic acid[AP5], Mg2) or protein kinase C inhibitor (H-7) causes the potentiation of morphine- induced antinociceptive action by using an in vivo electrophysiological technique. A single iontophoretic application of morphine or an antagonist alone induced weak inhibition of wide... -
Effects of Local Anesthetics on Rotational Mobility of n-(9-Anthroyloxy)stearic Acid in Neuronal Membranes
Hye-OckJang, ChangLee, Min-GakChoi, Sang-HunShin, In-KyoChung, IlYun 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.3 1 119-124 (6 pages)
To elucidate the molecular mechanism of pharmacological action of local anesthetics, we studied membrane actions of tetracaine, bupivacaine, lidocaine, prilocaine and procaine. Fluorescence polarization of n-(9-anthroyloxy)stearic acid (n-AS) was used to examine the effects of these local anesthetics on differential rotational mobility of different positions of the number of synaptosomal plasma membrane vesicle (SPMV) phospholipid carbon atoms. The four membrane components differed with respect... -
Mechanism of Glutamate-induced [Ca2+]i Increase in Substantia Gelatinosa Neurons of Juvenile Rats
SungJunJung, JeongSookChoi, JiyeonKwak, JunKim, JongWhanKim, SangJeongKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.2 1 53-57 (5 pages)
The glutamate receptors (GluRs) are key receptors for modulatory synaptic events in the central nervous system. It has been reported that glutamate increases the intracellular Ca2 concentration ([Ca2]i) and induces cytotoxicity. In the present study, we investigated whether the glutamate-induced [Ca2]i increase was associated with the activation of ionotropic (iGluR) and metabotropic GluRs (mGluR) in substantia gelatinosa neurons, using spinal cord slice of juvenile rats...
