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471.

Production of transgenic pigs using a pGFAP-CreERT2/EGFP LoxP inducible system for central nervous system disease models

Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 13 434-445 (12 pages)

Seon-Ung Hwang, Kiyoung Eun, Junchul David Yoon, Hyunggee Kim, Sang-Hwan Hyun 저
...
- 4,100원
Transgenic (TG) pigs are important in biomedical research and are used in disease modeling, pharmaceutical toxicity testing, and regenerative medicine. In this study, we constructed two vector systems by using the promoter of the pig glial fibrillary acidic protein (pGFAP) gene, which is an astrocyte cell marker. We established donor TG fibroblasts with pGFAP-CreERT2/LCMV-EGFPLoxP and evaluated the effect of the transgenes on TG-somatic cell nuclear transfer (SCNT) embryo development. Cleavage...
472.

Comparison of the effects of isoflurane versus propofolremifentanil anesthesia on oxygen delivery during thoracoscopic lung lobectomy with one-lung ventilation in dogs

Jiyoung Park, Hae-Beom Lee, Seong Mok Jeong 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 12 426-433 (8 pages)

Jiyoung Park, Hae-Beom Lee, Seong Mok Jeong 저
...
- 3,000원
This study compared effects of isoflurane inhalation (ISO) and propofol-remifentanil combined total intravenous anesthesia (TIVA) on oxygenation during thoracoscopic lung lobectomy with 30-min one-lung ventilation (1LV). Thoracoscopic right middle lung lobectomy was performed in ten dogs divided into ISO and TIVA groups, and cardiopulmonary parameters were measured with blood gas analysis. Throughout the study, isoflurane was inhaled up to 1.5%, and the infusion rates of propofol and...
473.

Identification, sequence analysis, and infectivity of H9N2 avian influenza viruses isolated from geese

Rui Zhu, Xueqin Yang, Jianjun Zhang, Danwen Xu, Jiawen Fan, Huoying Shi, Shifeng Wang, Xiufan Liu 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 10 406-415 (10 pages)

Rui Zhu, Xueqin Yang, Jianjun Zhang, Danwen Xu, Jiawen Fan, Huoying Shi, Shifeng Wang, Xiufan Liu 저
...
- 3,000원
The subtype H9N2 avian influenza virus greatly threatens the Chinese poultry industry, even with annual vaccination. Waterfowl can be asymptomatically infected with the H9N2 virus. In this study, three H9N2 virus strains, designated A/Goose/Jiangsu/YZ527/2011 (H9N2, Gs/JS/YZ527/11), A/Goose/Jiangsu/SQ119/2012 (H9N2, Gs/JS/SQ119/12), and A/Goose/Jiangsu/JD564/2012 (H9N2, Gs/JS/JD564/12), were isolated from domestic geese. Molecular characterization of the three isolates showed that the...
474.

Marek’s disease vaccine activates chicken macrophages

Dan Wang, Shuhong Sun, Mohammad Heidari 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 7 375-383 (9 pages)

Dan Wang, Shuhong Sun, Mohammad Heidari 저
...
- 3,000원
To provide insights into the role of innate immune responses in vaccine-mediated protection, we investigated the effect of Marek’s disease (MD) vaccine, CVI988/Rispens, on the expression patterns of selected genes associated with activation of macrophages in MD-resistant and MD-susceptible chicken lines. Upregulation of interferon , interleukin (IL)-1, IL-8, and IL-12 at different days post-inoculation (dpi) revealed activation of macrophages in both chicken lines. A strong...
475.

Phenotypic and genotypic analyses of an attenuated porcine reproductive and respiratory syndrome virus strain after serial passages in cultured porcine alveolar macrophages

Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang4, Chang 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 5 358-367 (10 pages)

Seung-Chul Lee, Sunhee Lee, Gun-Woo Yoo, Hwan-Won Choi, Yun-Hee Noh, Chang Eon Park, Jae-Ho Shin, In-Joong Yoon, Shien-Young Kang4, Chang 저
...
- 3,000원
The porcine reproductive and respiratory syndrome virus (PRRSV) is a globally ubiquitous swine viral pathogen that causes major economic losses worldwide. We previously reported an over-attenuated phenotype of cell-adapted PRRSV strain CA-2-P100 in vivo. In the present study, CA-2-P100 was serially propagated in cultured porcine alveolar macrophage (PAM) cells for up to 20 passages to obtain the derivative strain CA-2-MP120. Animal inoculation studies revealed that both CA-2-P100 and CA-2-MP120...
476.

Association between endotoxin levels in dust from indoor swine housing environments and the immune responses of pigs

Katharine Roque, Kyung Min Shin, Ji Hoon Jo, Gyeong Dong Lim, Eun Seob Song, So Jung Shin, Ravi Gautam, Jae Hee Lee, Yeon Gyeong Kim, Ah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 2 331-338 (8 pages)

Katharine Roque, Kyung Min Shin, Ji Hoon Jo, Gyeong Dong Lim, Eun Seob Song, So Jung Shin, Ravi Gautam, Jae Hee Lee, Yeon Gyeong Kim, Ah 저
...
- 3,000원
Indoor animal husbandry environments are inevitably contaminated with endotoxins. Endotoxin exposure is associated with various inflammatory illnesses in animals. This cross-sectional study evaluated the relationship between the degree of endotoxin exposure and the cellular and humoral immune profiles of fattening pigs. Blood samples were taken from the jugular vein of 47 pigs from ten pig farms in Korea. Whole blood cell counts and plasma immunoglobulin (Ig) classes were determined....
477.

Microglial involvement in the development of olfactory dysfunction

Yoojin Seo, Hyung-Sik Kim, Kyung-Sun Kang 대한수의학회 Journal of Veterinary Science 12 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 3호 1 319-330 (12 pages)

Yoojin Seo, Hyung-Sik Kim, Kyung-Sun Kang 저
...
- 4,100원
Olfactory impairment is the most common clinical manifestation among the elderly, and its prevalence increases sharply with age. Notably, growing evidence has shown that olfactory dysfunction is the first sign of neurodegeneration, indicating the importance of olfactory assessment an early marker in the diagnosis of neurological disorders. In this review, we describe the nature of olfactory dysfunction and the advantage of using animal models in olfaction study, and we include a brief...
478.

Detection of hepatitis E virus genotypes 3 and 4 in pig farms in Korea

Yong-Hyun Kim, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Hyeon-Jeong Go, Dong-Hwi Kim, Joong-Bok Lee, Seung-Yong Park, Chang- 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 18 309-312 (4 pages)

Yong-Hyun Kim, Byung-Joo Park, Hee-Seop Ahn, Sang-Hoon Han, Hyeon-Jeong Go, Dong-Hwi Kim, Joong-Bok Lee, Seung-Yong Park, Chang- 저
...
- 1,500원
Zoonotic transmission of hepatitis E virus (HEV) is mostly mediated by HEV-3 and HEV-4 genotypes, and domestic pigs are an important reservoir of these genotypes. A survey of 14 pig farms in Korea revealed HEV RNA in 30 of 148 (20.3%) fecal samples. HEV-3a and HEV-4c subtypes were identified in five pig farms (35.7%) and two pig farms (14.3%), respectively. Phylogenetic analysis indicated that the isolated HEV strains were closely related to previously reported zoonotic strains in Korea. The...
479.

Cytological endometritis in dairy cows: diagnostic threshold, risk factors, and impact on reproductive performance

Soo Chan Lee, Jae Kwan Jeong, In Soo Choi, Hyun Gu Kang, Young Hun Jung, Soo Bong Park, Ill Hwa Kim 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 17 301-308 (8 pages)

Soo Chan Lee, Jae Kwan Jeong, In Soo Choi, Hyun Gu Kang, Young Hun Jung, Soo Bong Park, Ill Hwa Kim 저
...
- 3,000원
We determined the threshold proportion of polymorphonuclear leukocytes (PMNs) for a diagnosis of cytological endometritis (CEM), the risk factors for this condition, and its impact on reproductive performance in dairy cows. Uterine cytology was performed on 407 Holstein cows 4 weeks postpartum to determine the proportions of endometrial cells and PMNs. A receiver operator characteristics curve was used to determine the threshold above which the PMN proportion affected the likelihood of cows...
480.

A two-component signal transduction system contributes to the virulence of Riemerella anatipestifer

Qing Wang, Mianmian Chen, Wei Zhang 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 12 260-270 (11 pages)

Qing Wang, Mianmian Chen, Wei Zhang 저
...
- 4,000원
Similar to other studies of bacterial pathogens, current studies of the pathogenesis of Riemerella anatipestifer (RA) are focused mainly on in vitro culture conditions. To elucidate further the pathogenesis of RA in vivo, bacterial RNA was extracted from overnight tryptic soy broth cultures (in vitro) and from the blood of infected ducks (in vivo) for comparative RNA sequencing analysis. In total, 682 upregulated genes were identified in vivo. Among the upregulated genes, a signal transduction...

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