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41.

Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity

Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)

Sang-Hyun Kim, Yong-Hwan Kim 저
...
- 3,000원
Escherichia coli (E. coli) has ability to express thin aggregative fimbriae, known as curli, on the cell surface. Previously, a few example of curli expression in serogroup O157:H7 of enterohemorrhagic E. coli (EHEC) were reported, compared to other E. coli groups. However, significance of curliation in the EHEC pathobiology has not been described well in the literature. A highly curliated O157:H7 strain was used in this study in order to elucidate role of curliation in EHEC adherence to...
42.

Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. Paratuberculosis

Sung Jae Shin, Han Sang Yoo, Sean P. McDonough, Yung-Fu Chang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 4 111-117 (7 pages)

Sung Jae Shin, Han Sang Yoo, Sean P. McDonough, Yung-Fu Chang 저
...
- 3,000원
Eighty-five complex (85A, 85B and 85C), 35-kDa and superoxide dismutase (SOD) were cloned, expressed and purified as antigens in an enzyme-linked immunosorbent assay (ELISA) to compare the serological reactivity of cows with different shedding levels of Mycobacterium avium subsp. paratuberculosis (MPT). Antibody responses to all recombinant antigens positively increased depending on shedding levels. In particular, antibody responses to the 35 kDa were higher than those to the others in all...
43.

Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae

Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 4 225-228 (4 pages)

Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 저
...
- 1,500원
sequences of apxIA gene was reported to GeneBank with the accession numbers of AF363361. Identity of the Apx IA from the cloned gene in E. coli was proved by SDS-PAGE and Western blot. Yeast has been demonstrated to be an excellent host for the expression of recombinant proteins with uses in diagnostics, therapeutics and vaccine productions. Therefore, to use the yeast as a delivery system in new oral subunit vaccine, apxIA gene was subcloned into Saccharomyces cerevisiae, and identified the...
44.

Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)

In-Soo Choi and Han-Sang Yoo 저
...
- 3,000원
Interleukin-2 plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by...
45.

Time Course Study of Cytokine mRNA Expression in LPS-Stimulated Porcine Alveolar Macrophages

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 2호 6 97-102 (6 pages)

In-Soo Choi, Na-Ri Shin, Sung-Jae Shin, Deog-Yong Lee, Young-Wook Choand Han-Sang Yoo 저
...
- 3,000원
and GM-CSF, IL-12 p35, and IL-12 p40 were 14, and 24 h, respectively. After reaching the peak expression point, the expression levels of IL-6, GM-CSF, and IL-12 p40 reduced to the baseline by 72 h after stimulation, however, IL-12 p35 still kept a substantial expression by the same time. This study demonstrates that porcine alveolar macrophages primarily respond to express IL-6, GM-CSF, and IL-12 by LPS-stimulation and have a cytokine-specific expression profile during the stimulation time.
46.

Expression and characterization of the flavoprotein domain of gp91phox

Chang-Hoon Han and Mun-Han Lee 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2000, 제 1권 제 1호 3 19-26 (8 pages)

Chang-Hoon Han and Mun-Han Lee 저
...
- 3,000원
a portion of the highly soluble bacterial protein thioredoxin (TRX). TRX-gp91(phox) (306-569), which contains the putative FAD and NADPH binding sites, showed NADPH-dependent NBT (nitroblue tetrazolium) reductase activity, whereas TRX-gp91(phox) (304-423) and TRX-gp91(phox) (424-569) were inactive. Activity saturated at about a 1:1 molar ratio of FAD to TRX-gp91(phox) (306- 569), and showed the same Km for NADPH as that for superoxide generating activity by the intact enzyme. Activity was not...
47.

Cytokine expression pattern in milk somatic cells of subclinical mastitis-affected cattle analyzed by real time PCR

Vaibhav D Bhatt , Prasad S Khade , Sagar B Tarate , Ajai K Tripathi , Dev S Nauriyal , Dharamshi N Rank , Anju P Kunjadia 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 8 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2012, 제 52권 제 4호 3 231-238 (8 pages)

Vaibhav D Bhatt , Prasad S Khade , Sagar B Tarate , Ajai K Tripathi , Dev S Nauriyal , Dharamshi N Rank , Anju P Kunjadia 저
...
- 3,000원
48.

Norepinephrine induces MAIL mRNA expression in primary cultured hepatocytes through IL-1β released from non-parenchymal cells

Hyeon Cheol Kim , Bae Dong Jung 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2010, 제 50권 제 2호 1 79-84 (6 pages)

Hyeon Cheol Kim , Bae Dong Jung 저
...
- 3,000원
49.

A STUDY ON THE EXPRESSION OF TYPE I AND TYPE II COLLAGEN GENES AND PROTEINS IN THE DEVELOPING HUMAN MANDIBLE

Yoon-Ah Kook, Sang-Cheol Kim, Eun-Cheol Kim 대한치과교정학회 The Korean Journal of Orthodontics 9 Pages

대한치과교정학회 The Korean Journal of Orthodontics 1995, 제 25권 제 6호 8 723-731 (9 pages)

Yoon-Ah Kook, Sang-Cheol Kim, Eun-Cheol Kim 저
...
- 무료
(RT-PCR), Western blot analysis, and Southern blot analysis. Our results showed that pro-α1(II) collagen gene expression begins in the 5th week. Type II collagen is synthesized in mesenchymal cells in advance of overt chondrogenesis. The gene expression for type II collagen was highest during the appearance of Meckel´s cartilage. There was a switch in collagen protein expression from type I to type II during the appearance stage of Meckel´s cartilage. The distribution of the mRNA...
50.

Time-dependent proteomic and genomic alterations in Toll-like receptor-4-activated human chondrocytes: increased expression of lamin A/C and annexins

SeungHeeHa, HyoungKyuKim, NguyenThiTuyetAnh, NariKim, KyungSooKo, ByoungDooRhee, JinHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 16 Pages

대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.5 8 531-546 (16 pages)

SeungHeeHa, HyoungKyuKim, NguyenThiTuyetAnh, NariKim, KyungSooKo, ByoungDooRhee, JinHan 저
...
- 4,500원
increased interleukin-1 beta (IL-1β), interleukin-6 (IL-6), and tumor necrosis factor alpha (TNF-α) expression levels. In TLR-4-activated chondrocytes, proteomic changes were determined by two dimensional electrophoresis and matrix-assisted laser desorption/ionization-mass spectroscopy analysis, and genomic changes were determined by microarray and gene ontology analyses. Proteomics analysis identified 26 proteins with significantly altered expression levels; these proteins were related to the...

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