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Rapid and Visual Detection of Barley Yellow Dwarf Virus by Reverse Transcription Recombinase Polymerase Amplification with Lateral Flow Strips
Na-Kyeong Kim, Hyo-Jeong Lee, Sang-Min Kim, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2022, 38권 2호 8 159-166 (8 pages)
Barley yellow dwarf virus (BYDV) has been a major viral pathogen causing significant losses of cereal crops including oats worldwide. It spreads naturally through aphids, and a rapid, specific, and reliable diagnostic method is imperative for disease monitoring and management. Here, we established a rapid and reliable method for isothermal reverse transcription recombinase polymerase amplification (RT-RPA) combined with a lateral flow strips (LFS) assay for the detection of BYDV-infected oat... -
Identification of Plant Viruses Infecting Pear Using RNA Sequencing
Nam-Yeon Kim, Hyo-Jeong Lee, Hong-Sup Kim, Su-Heon Lee, Jae-Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 3호 5 258-267 (10 pages)
Asian pear (Pyrus pyrifolia) is a widely cultivated and commercially important fruit crop, which is occasion- ally subject to severe economic losses due to latent viral infections. Thus, the aim of the present study was to ex- amine and provide a comprehensive overview of virus populations infecting a major pear cultivar (‘Singo’) in Korea. From June 2017 to October 2019, leaf samples (n = 110) of pear trees from 35 orchards in five major pear-producing regions were collected and subjected to... -
Identification and Characterization of Xanthomonas arboricola pv. juglandis Causing Bacterial Blight of Walnuts in Korea
Hyun Sup Kim, Wonsu Cheon, Younmi Lee, Hyeok-Tae Kwon, Sang-Tae Seo, Kotnala Balaraju, Yongho Jeon 한국식물병리학회 The Plant Pathology Journal 15 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 2호 5 137-151 (15 pages)
The present study describes the bacterial blight of wal- nut, caused by Xanthomonas arboricola pv. juglandis (Xaj) in the northern Gyeongbuk province, Korea. Dis- ease symptoms that appear very similar to anthracnose symptoms were observed in walnut trees in June 2016. Pathogens were isolated from disease infected leaves, fruits, shoots, bud, flower bud of walnut, and cultured onto yeast dextrose carbonate agar plates. Isolated bac- teria with bacterial blight symptoms were character- ized for... -
Variability in the Viral Protein Linked to the Genome of Turnip Mosaic Virus Influences Interactions with eIF(iso)4Es in Brassica rapa
Guoliang Li, Shifan Zhang, Fei Li, Hui Zhang, Shujiang Zhang, Jianjun Zhao, Rifei Sun 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 1호 4 47-56 (10 pages)
Plants protect against viruses through passive and ac- tive resistance mechanisms, and in most cases charac- terized thus far, natural recessive resistance to potyvi- ruses has been mapped to mutations in the eukaryotic initiation factor eIF4E or eIF(iso)4E genes. Five eIF4E copies and three eIF(iso)4E copies were detected in Brassica rapa. The eIF4E and eIF(iso)4E genes could interact with turnip mosaic virus (TuMV) viral protein linked to the genome (VPg) to initiate virus translation. From... -
Antagonistic Activity of Bacteria Isolated from Apple in Different Fruit Development Stages against Blue Mold Caused by Penicillium expansum
Roc?o Crystabel L?pez-Gonz?lez, Yara Suhan Ju?rez-Campusano, Jos? Luis Rodr?guez-Ch?vez, Guillermo Delgado-Lamas, Sof?a Mar?a Arvizu Medrano, Ram?n ?lvar Mart?nez-Peniche, Juan Ramiro Pacheco-Aguilar 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2021, 37권 1호 2 24-35 (12 pages)
Blue mold caused by Penicillium expansum is one of the most significant postharvest diseases of apples. Some microorganisms associated with the surface of ripen- ing apples possess the ability to inhibit the growth of P. expansum. However, the existing literature about their colonization in the stages before ripening is not explored in depth. This study aims to characterize the antagonistic capacity of bacterial populations from five fruit development stages of ‘Royal Gala’ apples. The re-... -
Characterization of Hibiscus Latent Fort Pierce Virus-Derived siRNAs in Infected Hibiscus rosa-sinensis in China
Han-hong Lan, Luan-mei Lu 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 6호 9 618-627 (10 pages)
Although limited progress have been made about pathogen system of Hibiscus rosa-sinensis and Hibiscus latent Fort Pierce virus (HLFPV), interaction between plant host and pathogen remain largely unknown, which led to deficiency of effective measures to control disease of hibiscus plants caused by HLFPV. In this study, infection of HLFPV in Hibiscus rosa-sinensis was firstly confirmed for the first time by traditional electron microscopy, modern reverse transcription polymerase chain reaction and... -
Characterization of a Strain of Malva Vein Clearing Virus in Alcea rosea via Deep Sequencing
Defu Wang, Liyan Cui, Yanni Pei, Zhennan Ma, Shaofei Shen, Dandan Long, Lingyu Li, Yanbing Niu 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 5호 9 468-475 (8 pages)
Malva vein clearing virus (MVCV) is a member of the Potyvirus species, and has a negative impact on the aesthetic development of Alcea rosea. It was first reported in Germany in 1957, but its complete genome sequence data are still scarce. In the present work, A. rosea leaves with vein-clearing and mosaic symptoms were sampled and analyzed with small RNA deep sequencing. By denovo assembly the raw sequences of virus-derived small interfering RNAs (vsiRs) and whole genome amplification of malva... -
Loop-Mediated Isothermal Amplification for the Detection of Xanthomonas arboricola pv. pruni in Peaches
Weilan Li, Seung-Yeol Lee, Chang-Gi Back, Leonid N. Ten, Hee-Young Jung 한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 9 635-643 (9 pages)
To detect Xanthomonas arboricola pv. pruni, a loopmediated isothermal amplification (LAMP) detection method were developed. The LAMP assay was designed to test crude plant tissue without pre-extraction, or heating incubation, and without advanced analysis equipment. The LAMP primers were designed by targeting an ABC transporter ATP-binding protein, this primer set was tested using the genomic DNA of Xanthomonas and non-Xanthomonas strains, and a ladder product was generated from the genomic DNA... -
siRNAs Derived from Cymbidium Mosaic Virus and Odontoglossum Ringspot Virus Down-modulated the Expression Levels of Endogenous Genes in Phalaenopsis equestris
Han-hong Lan, Cui-mei Wang, Shuang-shuang Chen, Jian-ying Zheng 한국식물병리학회 The Plant Pathology Journal 13 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 11 508-520 (13 pages)
Interplay between Cymbidium mosaic virus (CymMV)/ Odontoglossum ringspot virus (ORSV) and its host plant Phalaenopsis equestris remain largely unknown, which led to deficiency of effective measures to control disease of P. equestris caused by infecting viruses. In this study, for the first time, we characterized viral small interfering RNAs (vsiRNAs) profiles in P. equestris co-infected with CymMV and ORSV through small RNA sequencing technology. CymMV and ORSV small interfering RNAs (siRNAs)... -
Five Newly Collected Turnip Mosaic Virus (TuMV) Isolates from Jeju Island, Korea are Closely Related to Previously Reported Korean TuMV Isolates but Show Distinctive Symptom Development
Wen-Xing Hu, Byoung-Jo Kim, Younghwan Kwak, Eun-Young Seo, Jung-Kyu Kim, Jae-Yeong Han, Ik-Hyun Kim, Yong Pyo Lim, In-Sook Cho, Leslie L Domier, John Hammond, Hyoun-Sub Lim 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 10 381-387 (7 pages)
For several years, temperatures in the Korean peninsula have gradually increased due to climate change, resulting in a changing environment for growth of crops and vegetables. An associated consequence is that emerging species of insect vector have caused increased viral transmission. In Jeju Island, Korea, occurrences of viral disease have increased. Here, we report characterization of five newly collected turnip mosaic virus (TuMV) isolates named KBJ1, KBJ2, KBJ3, KBJ4 and KBJ5 from a survey...
