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551.

Biological characteristics of Chinese hamster ovary cells transfected with bovine Prnp

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 5 131-137 (7 pages)

Sang-Gyun Kang, Deog-Yong Lee, Mi Lan Kang, Han Sang Yoo 저
...
- 3,000원
detected in either transfectants or CHO-K1 cells. In SOD assay under ConA stimulation, the SOD activity of transfectants was 10 times higher than that of CHO-K1 cells at 6 h after treatment (p < 0.05). The genomic DNA of both the transfectants and control cells began to be fragmented at 6 h after treatment with cyclohexamide. Caspase-3 activity was reduced by transfection with the bovine Prnp (p < 0.05). Conclusively, the viability of transfectants expressing exogenous bovine Prnp was decreased...
552.

Diversity of swine Bordetella bronchiseptica isolates evaluated by RAPD analysis and PFGE

Eun-Kyung Shin, Yeon-Soo Seo, Jeong Hee Han, Tae-Wook Hahn 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 10 65-73 (9 pages)

Eun-Kyung Shin, Yeon-Soo Seo, Jeong Hee Han, Tae-Wook Hahn 저
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- 3,000원
The degree of genetic diversity in 45 Bordetella (B.) bronchiseptica strains comprised of a vaccine strain (N = 1), reference strains (N = 3) and field isolates (N = 41) was evaluated using random amplified polymorphic DNA (RAPD) fingerprinting and pulsed-field gel electrophoresis (PFGE). Three candidate primers were selected for RAPD analysis after screening 20 random decamer oligonucleotides for their discriminatory abilities. The OPA-07, OPA-08 and OPA-18 primers yielded 10, 10, and 6...
553.

Development of a monoclonal antibody-based co-agglutination test to detect enterotoxigenic Escherichia coli isolated from diarrheic neonatal calves

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 9 57-64 (8 pages)

Brajesh C. Varshney, N.M. Ponnanna, Pranati A. Sarkar, Pragna Rehman, Jigar H. Shah 저
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- 3,000원
Escherichia coli (E. coli) strains were collected from young diarrheic calves in farms and field. Strains that expressed the K99 (F5) antigen were identified by agglutination tests using reference antibodies to K99 antigen and electron microscopy. The K99 antigen from a selected field strain (SAR-14) was heat-extracted and fractionated on a Sepharose CL-4B column. Further purification was carried out by sodium deoxycholate treatment and/or ion-exchange chromatography. Monoclonal antibodies to...
554.

Detection of swine hepatitis E virus in the porcine hepatic lesion in Jeju Island

Song-Hak Lee, Sang-Chul Kang, Dae-Yong Kim, Jong-Hee Bae, Jae-Hoon Kim 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 8 51-55 (5 pages)

Song-Hak Lee, Sang-Chul Kang, Dae-Yong Kim, Jong-Hee Bae, Jae-Hoon Kim 저
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- 1,500원
by reverse transcriptase polymerase chain reaction (RT-PCR). In total, 40 pigs with hepatitis lesions were selected from 19 different farms, based on examination by microscopy. RTPCR findings revealed swine HEV in 22 cases (55%), including 18 suckling pigs and 4 growing pigs. Several histopathological lesions, including multifocal lymphoplasmacytic hepatitis, portal inflammation, and focal hepatocellular necrosis, were observed in liver sections of swine HEV PCR-positive pigs. The present study...
555.

Radioprotective effects of an acidic polysaccharide of Panax ginseng on bone marrow cells

Hyun-Ji Kim, Mi-Hyoung Kim, Yun-Young Byon, Jae Woo Park, Youngheun Jee, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 6 39-44 (6 pages)

Hyun-Ji Kim, Mi-Hyoung Kim, Yun-Young Byon, Jae Woo Park, Youngheun Jee, Hong-Gu Joo 저
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- 3,000원
T lymphocyte proliferation. Furthermore, APG-treated mice had a larger number of BMs after gamma radiation than the control mice, and the BMs of APG-treated mice were successfully cultured into dendritic cells, which are the representative antigenpresenting cells. Overall, this study shows that APG alters the phenotype of BMs, increases the viability and alloreactivity of BMs after gamma radiation both in vitro and in vivo. Therefore, APG may be a good candidate radioprotective agent for BMs.
556.

Organotypic slice culture of the hypothalamic paraventricular nucleus of rat

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 3 15-20 (6 pages)

Eun Seong Cho, So Yeong Lee, Jae-Yong Park, Seong-Geun Hong, Pan Dong Ryu 저
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- 3,000원
Organotypic slice cultures have been developed as an alternative to acute brain slices because the neuronal viability and synaptic connectivity in these cultures can be preserved well for a prolonged period of time. This study evaluated a stationary organotypic slice culture developed for the hypothalamic paraventricular nucleus (PVN) of rat. The results showed that the slice cultures maintain the typical shape of the nucleus, the immunocytochemical signals for oxytocin, vasopressin, and...
557.

Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates

Dennis Kaw Gomez, Dong Joo Lim, Gun Wook Baeck, Hee Jeong Youn, Nam Shik Shin, Hwa Young Youn, Cheol Yong Hwang, Jun Hong Park, 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 11 369-374 (6 pages)

Dennis Kaw Gomez, Dong Joo Lim, Gun Wook Baeck, Hee Jeong Youn, Nam Shik Shin, Hwa Young Youn, Cheol Yong Hwang, Jun Hong Park, 저
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- 3,000원
of the invertebrates were examined by reverse transcriptasepolymerase chain reaction (RT-PCR) and nested PCR to detect betanodavirus. Positive nested PCR results were obtained from the brains of 8 marine fish species (shrimp fish Aeoliscus strigatus, milkfish Chanos chanos, three spot damsel Dascyllus trimaculatus, Japanese anchovy Engraulis japonicus, pinecone fish Monocentris japonica, blue ribbon eel Rhinomuraena quaesita, look down fish Selene vomer, yellow tang Zebrasoma flavesenes), 1...
558.

Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens

Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 10 361-368 (8 pages)

Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 저
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- 3,000원
in all groups except for the normal control group showed the induction of antibodies to the IBDV at day 10 after the challenge. As judged by the lymphocyte proliferation assays using the a WST-8 solution performed on the peripheral blood and splenic lymphocytes, the stimulation indices (SI) of the peripheral blood lymphocytes in all groups except for the normal control group were similar immediately before the challenge. At 10 days post-challenge, the SI for DNA vaccine plus either CpG-ODN or...
559.

Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea

Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 8 349-354 (6 pages)

Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 저
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- 3,000원
a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to determine its prevalence and no program has been developed to control the disease. In this study, the sera of beef (n = 1,056) and dairy cattle (n = 1,105) from all provinces in Korea were tested to determine the prevalence of PTB using two different ELISA: an ‘in house’ modified absorbed ELISA (P-ELISA) based on sonicated antigen...
560.

Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry

Meejung Ahn, Heechul Kim, Jeong Tae Kim, Jeeyoung Lee, Jin Won Hyun, Jae Woo Park, Taekyun Shin 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 1 309-314 (6 pages)

Meejung Ahn, Heechul Kim, Jeong Tae Kim, Jeeyoung Lee, Jin Won Hyun, Jae Woo Park, Taekyun Shin 저
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- 3,000원
1 PI (p < 0.05), and returned to the level of normal control rats on days 4 and 9 PI. Immunohistochemistry showed that caveolin-1 immunoreactivity was enhanced in some glial cells, vascular endothelial cells, and neurons in the spinal cords. The increased expression of glial fibrillary acidic protein (GFAP), a marker for an astroglial reaction, was consistent with that of caveolin-1. In addition, caveolin-1 was co-localized in hypertrophied GFAP-positive astrocytes. Taking all these facts into...

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