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In vitro and in vivo gene therapy with CMV vector-mediated presumed dog β-nerve growth factor in pyridoxine-induced neuropathy dogs
Jin-Young Chung, Jung-Hoon Choi, Il-Seob Shin, Eun-Wha Choi, Cheol-Yong Hwang, Sang-Koo Lee, Hwa-Young Youn* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 5 367-374 (8 pages)
to the dog β-NGF. The recombinant pdβ-NGF plasmid was administrated into the intrathecal space in the gene therapy group. Twenty-four hours after the vector inoculation, the gene therapy group and the positive control group were intoxicated with excess pyridoxine for seven days. Each morning throughout the test period, the dogs' body weight was taken and postural reaction assessments were made. Electrophysiological recordings were performed twice, once before the experiment and once after the... -
Percutaneous heartworm removal from dogs with severe heart worm (Dirofilaria immitis) infestation
Seung-Gon Lee, Hyeong-Sun Moon, Changbaig Hyun* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 2호 11 197-202 (6 pages)
Canine heart worm disease is often life-threatening due to its various complications, including right side heart failure, caval syndrome and pulmonary eosinophilic granulomatosis. Several preventive medications and melarsomine have been developed and they are very effective to control heartworm infestation. However, in a case of severe infestation, melarsomine therapy often results in an unfavorable outcome because of the severe immune reaction caused by rapid killing of the adult worm. Surgical... -
Autologous somatic cell nuclear transfer in pigs using recipient oocytes and donor cells from the same animal
Eunsong Lee*, Kilyoung Song 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 15 415-422 (8 pages)
The objective of the present study was to examine the feasibility of the production of autologous porcine somatic cell nuclear transfer (SCNT) blastocysts using oocytes and donor cells from slaughtered ovaries. Therefore, we attempted to optimize autologous SCNT by examining the effects of electrical fusion conditions and donor cell type on cell fusion and the development of SCNT embryos. Four types of donor cells were used: 1) denuded cumulus cells (DCCs) collected from in vitro-matured (IVM)... -
The determination of dark adaptation time using electroretinography in conscious Miniature Schnauzer dogs
Hyung-Ah Yu, Man-Bok Jeong, Shin-Ae Park, Won-Tae Kim, Se-Eun Kim, Je-Min Chae, Na-Young Yi, Kang-Moon Seo* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 14 409-414 (6 pages)
The optimal dark adaptation time of electroretinograms (ERG's) performed on conscious dogs were determined using a commercially available ERG unit with a contact lens electrode and a built-in light source (LED-electrode). The ERG recordings were performed on nine healthy Miniature Schnauzer dogs. The bilateral ERG's at seven different dark adaptation times at an intensity of 2.5 cdㆍs/m² was performed. Signal averaging (4 flashes of light stimuli) was adopted to reduce electrophysiologic... -
Comparison of different bronchial closure techniques following pneumonectomy in dogs
Hakan Salci*, A. Sami Bayram, Ozgur Ozyigit, Cengiz Gebitekin, O. Sacit Gorgul 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 12 393-400 (8 pages)
and group II (GII) (n=9). Right and left pneumonectomies were performed on the animals in GI and GII, respectively. Each group was further divided into three subgroups according to BS closure technique: subgroup I (SGI) (n=3), manual suture; subgroup II (SGII) (n=3), stapler; and subgroup III (SGIII) (n=3), manual suture plus tissue flab. The dogs were sacrificed after one month of observation, and the bronchial stumps were removed for histological examination. The complications observed during... -
Successful introduction of modified dorsolumbar epidural anesthesia in a bovine referral center
Miho Hiraoka, Toshiharu Miyagawa, Hayato Kobayashi, Toshihiko Takahashi, Hiroaki Kishi, Hiroshi Kobayashi, Inhyung Lee 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 13 181-184 (4 pages)
3 ml of lidocaine. Infiltration anesthesia was performed in 22 cattle whose epidural space could not be reached in order to perform the flank surgery. The surgeries began about 12 min after the administration of the anesthetic and lasted for about 36 min. The modified method using a fixed volume of anesthetic was successfully introduced and effectively used in a bovine referral center. This modified method will allow veterinarians to save time and effort, thus lowering the cost of each surgery. -
Establishment of a canine spinal cord injury model induced by epidural balloon compression
Ji-Hey Lim, Chang-Su Jung, Ye-Eun Byeon, Wan Hee Kim, Jung-Hee Yoon, Kyung-Sun Kang, Oh-kyeong Kweon 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 13 89-94 (6 pages)
3-french embolectomy catheter was inserted into the epidural space through a left hemilaminectomy hole at the L4 vertebral arch. Balloons were then inflated with 50, 100, or 150 μl of a contrast agent at the L1 level for 6, 12, or 24 h and spinal canal occlusion (SCO) measured using computed tomography. Olby score was used to evaluate the extent of spinal cord injury and a histopathologic examination was conducted 1 week after surgery. The SCO of the 50, 100, and 150 μl inflations was 22-46%,... -
Modification of maturation condition improves oocyte maturation and in vitro development of somatic cell nuclear transfer pig embryos
Kilyoung Song, Eunsong Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 1호 12 81-87 (7 pages)
This study examined effects on the developmental competence of pig oocytes after somatic cell nuclear transfer (SCNT) or parthenogenetic activation (PA) of : 1) co-culturing of oocytes with follicular shell pieces (FSP) during in vitro maturation (IVM); 2) different durations of maturation; and 3) defined maturation medium supplemented with polyvinyl alcohol (PVA; control), pig follicular fluid (pFF), cysteamine (CYS), or β-mercaptoethanol (β-ME). The proportion of metaphase II oocytes was... -
Echocardiographic indices in normal German shepherd dogs
Ruthnea Aparecida Lazaro Muzzi, Leonardo Augusto Lopes Muzzi, Roberto Baracat de Araujo, Marcos Cherem 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 17 193-198 (6 pages)
Sixty clinically normal German shepherd dogs, 31 males and 29 females, ranging in age from 1 and 5 years and with a body weight ranging from 22 to 37.2 kg, were examined by the two-dimensional mode, M-mode, and Doppler echocardiography. In Doppler mode, the mitral valve flows were obtained, where the aim was to determine the velocity peaks and ratios of the E and A waves and the mitral E wave deceleration time. The velocity peaks were obtained for the tricuspid, pulmonary and aortic valves. On... -
Evaluation of a Bacillus stearothermophilus tube test as a screening tool for anticoccidial residues in poultry
Anakalo Shitandi, Aila Oketch, Symon Mahungum 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 14 177-180 (4 pages)
A Bacillus stearothermophilus var. calidolactis C953 tube test was evaluated for its ability in detecting the residue of selected anticoccidial drugs in poultry, specically sulfamethazine, furazolidone, and amprolium. Various concentrations of each drug were injected into chicken liver and kidney tissues and these tissues were tested to determine the drug detection limits for each drug. The detection limit was defined as the drug concentration at which 95% of the test results were interpreted as...


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