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51.

Effects of DDA, CpG-ODN, and plasmid-encoded chicken IFN-γ on protective immunity by a DNA vaccine against IBDV in chickens

Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 10 361-368 (8 pages)

Ha Jung Roh, Haan Woo Sung, Hyuk Moo Kwon 저
...
- 3,000원
This study examined the adjuvant effects of dimethyl dioctadecyl ammonium bromide (DDA), CpG oligodeoxynucleotides (CpG-ODN), and chicken interferon-γ (ChIFN-γ) on a DNA vaccine (pcDNA-VP243) against the infectious bursal disease virus (IBDV). A plasmid encoding chicken IFN-a was constructed. Twice at 2-week intervals, twoweek- old chickens were injected intramuscularly and intraperitoneally with either a DNA vaccine alone or a DNA vaccine together with the respective adjuvants. On week 2...
52.

Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea

Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 8 349-354 (6 pages)

Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 저
...
- 3,000원
Paratuberculosis (PTB) is a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to determine its prevalence and no program has been developed to control the disease. In this study, the sera of beef (n = 1,056) and dairy cattle (n = 1,105) from all provinces in Korea were tested to determine the prevalence of PTB using two different ELISA: an ‘in house’ modified absorbed ELISA (P-ELISA)...
53.

pH-dependent modulation of intracellular free magnesium ions with ion- selective electrodes in papillary muscle of guinea pig

Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 5 31-36 (6 pages)

Shang-Jin Kim, In-Gook Cho, Hyung-Sub Kang, Jin-Shang Kim 저
...
- 3,000원
A change in pH can alter the intracellular concentration of electrolytes such as intracellular Ca2+ and Na+ ([Na+]i) that are important for the cardiac function.For the determination of the role of pH in the cardiac magnesium homeostasis, the intracellular Mg 2+ concentration ([Mg 2+]i), membrane potential and contraction in the papillary muscle of guinea pigs using ion-selective electrodes changing extracellular pH ([pH]o)or intracellular pH ([pH ]i )were measured in this study. A high...
54.

Development of synthetic peptide ELISA based on nonstructural protein 2C of foot and mouth disease virus

Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Jong Hyeon Park, Yong Joo Kim, Hee Jong Song, Max Yeh 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 10 317-325 (9 pages)

Jae Ku Oem, Soo Jeong Kye, Kwang Nyeong Lee, Jong Hyeon Park, Yong Joo Kim, Hee Jong Song, Max Yeh 저
...
- 3,000원
It was reported that the sera of convalescent animals contain antibodies to foot and mouth disease (FMD) virus (FMDV) 2C, highly conserved nonstructural protein (NSP), whereas the sera of vaccinated animals do not. But ELISA methods using this protein were not reported and developed until recently. In this study, NSP 2C peptides were synthesized within the amino acid sequence of the conserved 2C nonstructural region of FMDV according to the sequences from Genbank database and used for...
55.

Validation of an electrometric blood cholinesterase measurement in goats

M. M.H. Al-Jobory, F. K. Mohammad 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 7 299-303 (5 pages)

M. M.H. Al-Jobory, F. K. Mohammad 저
...
- 1,500원
as a substrate. The mixture was incubated at 37 degrees C for 40 minutes. The pH of the reaction mixture was determined by a pH meter before and after the incubation. The initial pH was measured before the substrate addition. The enzyme activity was expressed as deltapH/40 min. The coefficients of variation of the described method in measuring plasma and erythrocyte cholinesterase activities were 4 and 2%, respectively. Preliminary reference values (n = 14) of the mean cholinesterase activity...
56.

Development of a novel antigen capture-ELISA using IgY against porcine interleukin-6 and its application

Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 9 337-343 (7 pages)

Deog Yong Lee, Young Wook Cho, Sang Gyun Kang, Sung Jae Shin, Han Sang Yoo 저
...
- 3,000원
Interleukin-6 (IL-6) is introduced as a marker of disease. At present, a variety of method may be used to quantify expression of this protein. Antigen capture-ELISA is a sensitive and accurate quantification method previously used with ovine, rat, and human IL-6 proteins. However, it has never been reported to quantify porcine IL-6 protein using capture ELISA. In this study, we generated and characterized a set of IgY and mono-specific polyclonal antibodies to recombinant porcine IL-6 (rpIL-6),...
57.

Identification of a putative cellular receptor 150 kDa polypeptide for porcine epidemic diarrhea virus in porcine enterocytes

Jin Sik Oh, Dae Sub Song, Bong Kyun Park 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 12 269-275 (7 pages)

Jin Sik Oh, Dae Sub Song, Bong Kyun Park 저
...
- 3,000원
Porcine epidemic diarrhea virus (PEDV) causes an acute enteritis in pigs of all ages, often fatality for neonates. PEDV occupies an intermediate position between two well characterized members of the coronavirus group I, human coronavirus (HCoV-229E) and transmissible gastroenteritis virus (TGEV) which uses aminopeptidase N (APN), a 150 kDa protein, as their receptors. However, the receptor of the PEDV has not been identified yet. A virus overlay protein binding assay (VOPBA) was used to...
58.

Expression of apxIA of Actinobacillus pleuropneumoniae in Saccharomyces cerevisiae

Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2003, 제 4권 제 3호 4 225-228 (4 pages)

Sung Jae Shin, Jong Lye Bae, Young Wook Cho, Moon Sik Yang, Dae Hyuk Kim, Yong Suk Jang, Han Sang Yoo 저
...
- 1,500원
Actinobacillus pleuropneumoniae is an important primary pathogen in pigs, in which it causes a highly contagious pleuropneumoniae. In our previous study, apxIA gene amplified from A. pleuropneumoniae Korean isolate by PCR with primer designed based on the N- and C-terminal of the toxin was cloned in TA cloning vector and sequenced. The nucleotide sequences of apxIA gene was reported to GeneBank with the accession numbers of AF363361. Identity of the Apx IA from the cloned gene in E. coli was...
59.

Development and Evaluation of a New Apparatus for Continuous Perfusion of Isolated Perfused Pig Heart

Mi-Young An, Emmanuelle P. Canel, In-Ho Jang, Didier Revel, Theresa W. Fossum, Nam-Sik Chungand Marc F. Janier 대한수의학회 Journal of Veterinary Science 14 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 12 219-232 (14 pages)

Mi-Young An, Emmanuelle P. Canel, In-Ho Jang, Didier Revel, Theresa W. Fossum, Nam-Sik Chungand Marc F. Janier 저
...
- 4,300원
To develop a better model of isolated perfused heart, a new apparatus of coronary artery cannula- fixed-in-aortic tube was developed for continuous normothermic perfusion and compared to the Casalis apparatus with cold ischemia. Eight mongrel pigs with the body weight of 18 to 24 kg were divided half into two groups. All the continuous perfusion experimental hearts resumed a spontaneous heart beat and stabilized earlier than the control hearts without the need of defibrillator or pacemaker,...
60.

Expression of Recombinant Porcine Interleukin-2 and Application of Its Antibody to Immunoassays

In-Soo Choi and Han-Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 10 207-212 (6 pages)

In-Soo Choi and Han-Sang Yoo 저
...
- 3,000원
plays an important role in T lymphocyte proliferation and immune response regulations. In this study, porcine IL-2 cDNA was cloned from peripheral blood mononuclear cells, and recombinant porcine IL-2 (rpIL-2) was expressed in Escherichia coli. The size of rpIL-2 without signal peptides was about 15 kDa when determined by SDS-PAGE and Western blotting analysis. Anti-rpIL-2 antibody was produced from mice immunized with the purified rpIL-2, and its specificity was examined by Western blotting and...

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