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51.

Paclitaxel inhibits the hyper-activation of spleen cells by lipopolysaccharide and induces cell death

Hyun-Ji Kim, Hong-Gu Joo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 4호 3 453-458 (6 pages)

Hyun-Ji Kim, Hong-Gu Joo 저
...
- 3,000원
anticancer agent. Paclitaxel prevents cancer cell division by inhibiting spindle fiber function, inducing cell death. A recent study demonstrated that paclitaxel binds to myeloid differentiation protein-2 of Toll-like receptor 4 and prevents the signal transduction of lipopolysaccharide (LPS). Paclitaxel converts immune cells hypo-responsive to LPS. In this study, we investigated whether paclitaxel can inhibit the phenotype and function of immune cells. To accomplish this, we used spleen cells,...
52.

Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model

Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)

Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 저
...
- 3,000원
cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated bovine mammary gland epithelial cells (bMGEs) were used as a...
53.

Rapamycin treatment during in vitro maturation of oocytes improves embryonic development after parthenogenesis and somatic cell nuclear transfer in pigs

Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 16 373-380 (8 pages)

Joohyeong Lee, Jong-Im Park, Jung Im Yun, Yongjin Lee, Hwanyul Yong, Seung Tae Lee, Choon-Keun Park, Sang-Hwan Hyun, Geun-Shik L 저
...
- 3,000원
This study was conducted to investigate the effects of rapamycin treatment during in vitro maturation (IVM) on oocyte maturation and embryonic development after parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) in pigs. Morphologically good (MGCOCs) and poor oocytes (MPCOCs) were untreated or treated with 1 nM rapamycin during 0–22 h, 22–42 h, or 0–42 h of IVM. Rapamycin had no significant effects on nuclear maturation and blastocyst formation...
54.

Developmental changes in cell proliferation and apoptosis in the normal duck bursa of Fabricius

Jing Fang, Xi Peng 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 2 465-474 (10 pages)

Jing Fang, Xi Peng 저
...
- 3,000원
The aim of this work was to investigate developmental changes in cell proliferation and apoptosis in normal duck bursa of Fabricius using flow cytometry and immunohistochemistry. Studies were carried out on Tianfu ducks on days 24 and 27 of embryogenesis (E24 and E27) along with days 20, 70, and 200 of postnatal development (P20, P70, and P200). Results showed that the percentage of G0/G1 bursa cells significantly increased between E24 and P200 while the percentage of cells in the S phase or G2...
55.

Cloned calves derived from somatic cell nuclear transfer embryos cultured in chemically defined medium or modified synthetic oviduct fluid

Goo Jang*, So Gun Hong, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 12 83-89 (7 pages)

Goo Jang*, So Gun Hong, Byeong Chun Lee* 저
...
- 3,000원
Somatic cell nuclear transfer (SCNT) is considered to be a critical tool for propagating valuable animals. To determine the productivity calves resulting from embryos derived with different culture media, enucleated oocytes matured in vitro were reconstructed with fetal fibroblasts, fused, and activated. The cloned embryos were cultured in modified synthetic oviduct fluid (mSOF) or a chemically defined medium (CDM) and developmental competence was monitored. After 7 days of culturing, the...
56.

Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Mingeun Sagong, Choi-Kyu Park, Seong-Hee Kim, Sung-Up Moon, Seong-Cheol Cho, Changhee Lee 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 13 169-171 (3 pages)

Mingeun Sagong, Choi-Kyu Park, Seong-Hee Kim, Sung-Up Moon, Seong-Cheol Cho, Changhee Lee 저
...
- 1,500원
industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.
57.

Microtubule distribution in somatic cell nuclear transfer bovine embryos following control of nuclear remodeling type

Dae-Jin Kwon, Yu-Mi Lee, In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 1 93-101 (9 pages)

Dae-Jin Kwon, Yu-Mi Lee, In-Sun Hwang, Choon-Keun Park, Boo-Keun Yang, Hee-Tae Cheong 저
...
- 3,000원
not disappear until 3 h after fusion, regardless of treatment (82.9-87.2%). A significantly high proportion of embryos showing an abnormal chromosome or microtubule distribution was observed in the roscovitine- treated group (40.0%, p ＜ 0.05) compared to the caffeine- treated group (22.1%). In conclusion, PCC is a favorable condition for the normal organization of microtubules, and inhibition of PCC can cause abnormal mitotic division of bovine SCNT embryos by causing microtubule dysfunction.
58.

Fatal cases of Theileria annulata infection in calves in Portugal associated with neoplastic-like lymphoid cell proliferation

Sandra Branco, Jo?o Orvalho, Alexandre Leit?o, Isadora Pereira, Manuel Malta, Isabel Mariano, T?nia Carvalho, Rui Baptista, Bria 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 5 27-34 (8 pages)

Sandra Branco, Jo?o Orvalho, Alexandre Leit?o, Isadora Pereira, Manuel Malta, Isabel Mariano, T?nia Carvalho, Rui Baptista, Bria 저
...
- 3,000원
This study was carried out to investigate fifteen cases of acute lethal infection of calves (≤ 4 months of age) by the protozoan parasite Theileria (T.) annulata in the south of Portugal. Calves developed multifocal to coalescent nodular skin lesions, similar to multicentric malignant lymphoma. Infestation with ticks (genus Hyalomma) was intense. Theileria was seen in blood and lymph node smears, and T. annulata infection was confirmed by isolation of schizont-transformed cells and sequencing...
59.

Oocyte maturation under a biophoton generator improves preimplantation development of pig embryos derived by parthenogenesis and somatic cell nuclear transfer

Joohyeong Lee , Hyeji Shin , Wonyou Lee , Seung Tae Lee , Geun-shik Lee , Sang-hwan Hyun , Eunsong Lee 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 7 Pages

대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2017, 제 57권 제 2호 3 89-95 (7 pages)

Joohyeong Lee , Hyeji Shin , Wonyou Lee , Seung Tae Lee , Geun-shik Lee , Sang-hwan Hyun , Eunsong Lee 저
...
- 3,000원
60.

The p90rsk-mediated signaling of ethanol-induced cell proliferation in HepG2 cell line

HanSangKim, Su-JinKim, JinhyungBae, YiyiWang, SunYoungPark, YoungSilMin, HyunDongJe, UyDongSohn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages

대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.6 5 595-603 (9 pages)

HanSangKim, Su-JinKim, JinhyungBae, YiyiWang, SunYoungPark, YoungSilMin, HyunDongJe, UyDongSohn 저
...
- 3,000원
is known to be an important downstream kinase of p44/42 MAPK. We investigated the role of p90rsk on ethanol-induced cell proliferation of HepG2 cells. HepG2 cells were treated with 10~50 mM of ethanol with or without ERK and p90rsk inhibitors. Cell viability was measured by MTT assay. The expression of pERK1, NHE1 was measured by Western blots. The phosphorylation of p90rsk was measured by ELISA kits. The expression of Bcl-2 was measured by qRT-PCR. When the cells were treated with 10~30 mM of...

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