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51.

Development and evaluation of a competitive enzyme-linked immunosorbent assay using a monoclonal antibody for diagnosis of severe fever with thrombocytopenia syndrome virus in bovine sera

Hyojin Lee, Eun-Ju Kim, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, In-Soo Cho, Yeun-Kyung Shin 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 3호 6 307-314 (8 pages)

Hyojin Lee, Eun-Ju Kim, Jae-Young Song, Jeong Soo Choi, Ji Youn Lee, In-Soo Cho, Yeun-Kyung Shin 저
...
- 3,000원
Severe fever with thrombocytopenia syndrome (SFTS) caused by the SFTS virus (SFTSV), a phlebovirus in the family Bunyaviridae, is an emerging tick-borne infectious disease that impacts humans. This disease manifests as a decreased blood cell count and multi-organ failure, with a case-fatality rate of more than 12% in China. Because vaccines or antiviral drugs for the treatment of this disease are not available, monitoring the SFTS circulation in animals and controlling the tick-mammal cycle are...
52.

Increased humoral antibody response of foot-and-mouth disease virus vaccine in growing pigs pre-treated with poly-γ-glutamic acid

Jee-Hoon Lee, Ik-Jae Kang, A-Reum Kim, You-Sun Noh, Hee-Chun Chung, Bong-Kyun Park 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 15 253-256 (4 pages)

Jee-Hoon Lee, Ik-Jae Kang, A-Reum Kim, You-Sun Noh, Hee-Chun Chung, Bong-Kyun Park 저
...
- 1,500원
This study was conducted to determine if humoral antibody response of foot-and-mouth disease (FMD) vaccine improved in 8-week-old growing pigs born to well-vaccinated sows pre-treated with 60 mg of poly-γ-glutamic acid (γ-PGA) three days before vaccination. Antibody against FMD virus serotype O was measured 0, 2, 4 and 6 weeks post-vaccination, using a PrioCHECK FMDV type O ELISA kit. The results showed that positive antibody reactions against FMDV serotype O antigen among a component of the...
53.

Cloned foal derived from in vivo matured horse oocytes aspirated by the short disposable needle system

Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 15 509-516 (8 pages)

Wonyou Lee, Kilyoung Song, Inhyung Lee, Hyungdo Shin, Byeong Chun Lee, Seongchan Yeon, Goo Jang 저
...
- 3,000원
Transvaginal ultrasound-guided follicle aspiration is one method of obtaining recipient oocytes for equine somatic cell nuclear transfer (SCNT). This study was conducted: (1) to evaluate the possibility of oocyte aspiration from pre-ovulatory follicles using a short disposable needle system (14-G) by comparing the oocyte recovery rate with that of a long double lumen needle (12-G); (2) to investigate the developmental competence of recovered oocytes after SCNT and embryo transfer. The recovery...
54.

Dietary germanium biotite supplementation enhances the induction of antibody responses to foot-and-mouth disease virus vaccine in pigs

Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 15 443-447 (5 pages)

Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 저
...
- 1,500원
We evaluated the potential ability of germanium biotite (GB) to stimulate the production of antibodies specific for foot-and-mouth disease virus (FMDV). To this aim, we measured the total FMDV-specific antibody responses and IgM production after vaccination against FMD both experimentally and in the field. GB supplementation with FMDV vaccination stimulated the production of anti-FMDV antibodies, and effectively increased IFN-γ and TNF-α levels. These results suggest that GB may be a novel...
55.

Gossypol acetic acid induces apoptosis in RAW264.7 cells via a caspase-dependent mitochondrial signaling pathway

Sijun Deng, Hui Yuan, Jine Yi, Yin Lu, Qiang Wei, Chengzhi Guo, Jing Wu, Liyun Yuan, Zuping He 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 7 281-289 (9 pages)

Sijun Deng, Hui Yuan, Jine Yi, Yin Lu, Qiang Wei, Chengzhi Guo, Jing Wu, Liyun Yuan, Zuping He 저
...
- 3,000원
To investigate the effects of gossypol acetic acid (GA) on proliferation and apoptosis of the macrophage cell line RAW264.7 and further understand the possible underlying mechanism responsible for GA-induced cell apoptosis, RAW264.7 cells were treated with GA (25∼35 μmol/L) for 24 h and the cytotoxicity was determined by MTT assay, while apoptotic cells were identified by TUNEL assay, acridine orange/ethidium bromide staining and flow cytometry. Moreover, mitochondrial membrane potential...
56.

Development of monoclonal antibodies against the abnormal prion protein isoform (PrP) associated with chronic wasting disease (CWD)

Hyun-Jeong Jeong, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Kun-Ho Seo, Dong-Woon Kim, Yong-Sun Kim, In-So 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 14 429-432 (4 pages)

Hyun-Jeong Jeong, Nak-Hyung Lee, Joong-Bok Lee, Seung-Yong Park, Chang-Seon Song, Kun-Ho Seo, Dong-Woon Kim, Yong-Sun Kim, In-So 저
...
- 1,500원
Monoclonal antibodies (mAbs) specific for the abnormal prion protein isoform (PrP) are indispensable for diagnosing chronic wasting disease (CWD). In this study, eight mAbs were developed by immunizing PrP knockout mice with recombinant elk PrP and an immunogenic PrP peptide. The reactivity of the mAbs to recombinant PrP and the PrP peptide was measured, and their isotypes were subsequently determined. Among them, four mAbs (B85-05, B85-08, B85-12, and B77-75) were shown by Western blotting to...
57.

Peste des petits ruminants virus detected in tissues from an Asiatic lion (Panthera leo persica) belongs to Asian lineage IV

Vinayagamurthy Balamurugan, Arnab Sen, Gnanavel Venkatesan, Vandana Bhanot, Vineeta Yadav, Veerakyathappa Bhanuprakash, Raj Kuma 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 2호 13 203-206 (4 pages)

Vinayagamurthy Balamurugan, Arnab Sen, Gnanavel Venkatesan, Vandana Bhanot, Vineeta Yadav, Veerakyathappa Bhanuprakash, Raj Kuma 저
...
- 1,500원
In this study, peste des petits ruminants virus (PPRV) was detected in frozen pooled tissue samples from a dead Asiatic lion (Panthera leo persica). The samples were negative for canine distemper virus and positive for PPRV nucleic acids when tested with one-step RT-PCR using the appropriate virus-specific primers. Subsequent amplification, cloning, and sequencing of the partial nucleocapsid, matrix, and fusion genes confirmed the presence of PPRV nucleic acid. Comparative sequence and...
58.

Evaluation and comparison of native and recombinant LipL21 protein-based ELISAs for diagnosis of bovine leptospirosis

Siju Joseph*, Naicy Thomas, E. Thangapandian, Vijendra P Singh, Rishendra Verma, S. K. Srivastava 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 14 99-101 (3 pages)

Siju Joseph*, Naicy Thomas, E. Thangapandian, Vijendra P Singh, Rishendra Verma, S. K. Srivastava 저
...
- 1,500원
A 21-kDa leptospiral lipoprotein (LipL21) was evaluated for its diagnostic potential to detect bovine leptospirosis by ELISA. Both native LipL21 (nLipL21) and recombinant LipL21 (rLipL21) proteins were tested and compared regarding diagnostic efficiency, and no statistically significant difference was observed. The sensitivity of rLipL21 ELISA for 62 microscopic agglutination test (MAT) positive sera was 100% and the specificity with 378 MAT negative sera was 97.09%. Thus, rLipL21 protein-based...
59.

Generation of transgenic corn-derived Actinobacillus pleuropneumoniae ApxIIA fused with the cholera toxin B subunit as a vaccine candidate

Min-Kyoung Shin, Myung Hwan Jung, Won-Jung Lee, Pil Son Choi, Yong-Suk Jang, Han Sang Yoo* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 14 401-403 (3 pages)

Min-Kyoung Shin, Myung Hwan Jung, Won-Jung Lee, Pil Son Choi, Yong-Suk Jang, Han Sang Yoo* 저
...
- 1,500원
Corn, one of the most important forage crops worldwide, has proven to be a useful expression vehicle due to the availability of established transformation procedures for this well-studied plant. The exotoxin Apx, a major virulence factor, is recognized as a common antigen of Actinobacillus (A.) pleuropneumoniae, the causative agent of porcine pleuropneumonia. In this study, a cholera toxin B (CTB)-ApxIIA#5 fusion protein and full-size ApxIIA expressed in corn seed, as a subunit vaccine...
60.

Expression and regulation of Enpp2 in rat uterus during the estrous cycle

Hyo-Jin Ahn, Hyun Yang, Beum-Soo An, Kyung-Chul Choi, Eui-Bae Jeung* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 11 379-385 (7 pages)

Hyo-Jin Ahn, Hyun Yang, Beum-Soo An, Kyung-Chul Choi, Eui-Bae Jeung* 저
...
- 3,000원
Ectonucleotide pyrophosphatase/phosphodiestrase 2 (Enpp2) isolated from the supernatant of human melanoma cells is a lysophospholipase D that transforms lysophosphatidylcholine into lysophospatidic acid. Although multiple analyses have investigated the function of Enpp2 in the hypothalamus, its role in the uterus during the estrous cycle is not well understood. In the present study, rat uterine Enpp2 was analyzed by RT-PCR, Western blotting, and immunohistochemistry. Quantitative PCR analysis...

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