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51.

Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 12 575-579 (5 pages)

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 저
...
- 1,500원
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an...
52.

The Current Status and Future Outlook of Quantum Dot-Based Biosensors for Plant Virus Detection

한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 2호 1 85-92 (8 pages)

저
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- 3,000원
Enzyme-linked immunosorbent assay (ELISA) and polymerase chain reaction (PCR), widely used for the detection of plant viruses, are not easily performed, resulting in a demand for an innovative and more efficient diagnostic method. This paper summarizes the characteristics and research trends of biosensors focusing on the physicochemical properties of both interface elements and bioconjugates. In particular, the topological and photophysical properties of quantum dots (QDs) are discussed, along...
53.

Generation of an Infectious Clone of a New Korean Isolate of Apple chlorotic leaf spot virus Driven by Dual 35S and T7 Promoters in a Versatile Binary Vector

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 6호 10 608-613 (6 pages)

저
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- 3,000원
The full-length sequence of a new isolate of Apple chlorotic leaf spot virus (ACLSV) from Korea was divergent, but most closely related to the Japanese isolate A4, at 84% nucleotide identity. The full-length cDNA of the Korean isolate of ACLSV was cloned into a binary vector downstream of the bacteriophage T7 RNA promoter and the Cauliflower mosaic virus 35S promoter. Chenopodium quinoa was successfully infected using in vitro transcripts synthesized using the T7 promoter, detected at 20...
54.

Experimental Infection of Different Tomato Genotypes with Tomato mosaic virus Led to a Low Viral Population Heterogeneity in the Capsid Protein Encoding Region

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 5호 8 508-513 (6 pages)

저
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- 3,000원
The complete genome sequence of a Slovak SL-1 isolate of Tomato mosaic virus (ToMV) was determined from the next generation sequencing (NGS) data, further confirming a limited sequence divergence in this tobamovirus species. Tomato genotypes Monalbo, Mobaci and Moperou, respectively carrying the susceptible tm-2 allele or the Tm-1 and Tm-2 resistant alleles, were tested for their susceptibility to ToMV SL-1. Although the three tomato genotypes accumulated ToMV SL-1 to similar amounts as judged...
55.

A Novel Recombined Potato virus Y Isolate in China

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 4호 4 382-392 (11 pages)

저
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- 4,000원
This study reports the findings of a distinct Potato virus Y (PVY) isolate found in Northeast China. One hundred and ten samples (leaves and tubers) were collected from potato plants showing mosaic symptoms around the city of Harbin in Heilongjiang province of China. The collected tubers were planted and let to grow in a greenhouse. New potato plants generated from these tubers showed similar symptoms, except for one plant. Subsequent serological analyses revealed PVY as the causing agent of the...
56.

Efficient Transmission and Propagation of Tomato Chlorosis Virus by Simple Single-Leaflet Grafting

한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 3호 13 345-349 (5 pages)

저
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- 1,500원
Tomato chlorosis virus (ToCV), a member of the genus Crinivirus, has caused an epidemic disease in tomato worldwide. ToCV is phloem-limited and transmitted by whiteflies in a semi-persistent manner, but not by mechanical inoculation. Experimental propagation of ToCV has been performed primarily by using whitefly-mediated inoculation. To develop a simple and convenient method for transmission of ToCV, we investigated grafting single-leaflets from tomato plants infected with ToCV to recipient...
57.

A Model to Explain Temperature Dependent Systemic Infection of Potato Plants by Potato virus Y

한국식물병리학회 The Plant Pathology Journal 6 Pages

한국식물병리학회 The Plant Pathology Journal 2017, 33권 2호 12 206-211 (6 pages)

저
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- 3,000원
The effect of temperature on the rate of systemic infection of potatoes (Solanum tuberosum L. cv. Chu-Baek) by Potato virus Y (PVY) was studied in growth chambers. Systemic infection of PVY was observed only within the temperature range of 16°C to 32°C. Within this temperature range, the time required for a plant to become infected systemically decreased from 14 days at 20°C to 5.7 days at 28°C. The estimated lower thermal threshold was 15.6°C and the...
58.

Deep Sequencing Analysis of Apple Infecting Viruses in Korea

한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 8 441-451 (11 pages)

저
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- 4,000원
Deep sequencing has generated 52 contigs derived from five viruses; Apple chlorotic leaf spot virus (ACLSV), Apple stem grooving virus (ASGV), Apple stem pitting virus (ASPV), Apple green crinkle associated virus (AGCaV), and Apricot latent virus (ApLV) were identified from eight apple samples showing small leaves and/or growth retardation. Nucleotide (nt) sequence identity of the assembled contigs was from 68% to 99% compared to the reference sequences of the five respective viral genomes....
59.

Diversity Evaluation of Xylella fastidiosa from Infected Olive Trees in Apulia (Southern Italy)

한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2016, 32권 2호 4 102-111 (10 pages)

저
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- 3,000원
Olive culture is very important in the Mediterranean Basin. A severe outbreak of Olive Quick Decline Syndrome (OQDS) caused by Xylella fastidiosa infection was first noticed in 2013 on olive trees in the southern part of Apulia region (Lecce province, southern Italy). Studies were carried out for detection and diversity evaluation of the Apulian strain of Xylella fastidiosa. The presence of the pathogen in olive samples was detected by PCR amplifying the 16S rDNA, gyrase B subunit (gyrB) and HL...
60.

Development of a Rapid Detection Method for Potato virus X by Reverse Transcription Loop-Mediated Isothermal Amplification

한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2015, 31권 3호 3 219-225 (7 pages)

저
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- 3,000원
The primary step for efficient control of viral diseases is the development of simple, rapid, and sensitive virus detection. Reverse transcription loop-mediated isothermal amplification (RT-LAMP) has been used to detect viral RNA molecules because of its simplicity and high sensitivity for a number of viruses. RT-LAMP for the detection of Potato virus X (PVX) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to demonstrate its advantages over...

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