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591.

Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat

Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 2 11-18 (8 pages)

Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 저
...
- 3,000원
Gaeumannomyces graminis var. tritici is a soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression...
592.

Rapid and Specific Detection of Apple stem grooving virus by Reverse Transcription-recombinase Polymerase Amplification

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 한국식물병리학회 The Plant Pathology Journal 5 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 12 575-579 (5 pages)

Nam-Yeon Kim, Jong-hee Oh, Su-Heon Lee, Hong-sup Kim, Jae Sun Moon, Rae-Dong Jeong 저
...
- 1,500원
Apple stem grooving virus (ASGV) is considered to cause the most economically important viral disease in pears in Korea. The current PCR-based methods used to diagnose ASGV are time-consuming in terms of target detection. In this study, a novel assay for specific ASGV detection that is based on reverse transcription-recombinase polymerase amplification is described. This assay has been shown to be reproducible and able to detect as little as 4.7 ng/μl of purified RNA obtained from an...
593.

Complete Genome Sequences and Evolutionary Analysis of Cucurbit aphid-borne yellows virus Isolates from Melon in Korea

Hae-Ryun Kwak, Hee Ju Lee, Eun-A Kim, Jang-Kyun Seo, Chang-Seok Kim, Sang Gyu Lee, Jeong-Soo Kim, Hong-Soo Choi, Mi-kyeong Kim 한국식물병리학회 The Plant Pathology Journal 12 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 8 532-543 (12 pages)

Hae-Ryun Kwak, Hee Ju Lee, Eun-A Kim, Jang-Kyun Seo, Chang-Seok Kim, Sang Gyu Lee, Jeong-Soo Kim, Hong-Soo Choi, Mi-kyeong Kim 저
...
- 4,100원
Complete genome sequences of 22 isolates of Cucurbit aphid-borne yellows virus (CABYV), collected from melon plants showing yellowing symptom in Korea during the years 2013-2014, were determined and compared with previously reported CABYV genome sequences. The complete genomes were found to be 5,680-5,684 nucleotides in length and to encode six open reading frames (ORFs) that are separated into two regions by a non-coding internal region (IR) of 199 nucleotides. Their genomic organization is...
594.

A New Distinct Clade for Iranian Tomato spotted wilt virus Isolates Based on the Polymerase, Nucleocapsid, and Non-structural Genes

Mahsa Abadkhah, Davoud Koolivand, Omid Eini 한국식물병리학회 The Plant Pathology Journal 18 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 7 514-531 (18 pages)

Mahsa Abadkhah, Davoud Koolivand, Omid Eini 저
...
- 4,700원
leaf and fruit samples from tomato (Solanum lycopersicum) plants with viral symptoms were collected from the fields in various regions of Iran. ELISA test revealed that the samples were infected by TSWV. The results of RTPCR showed that the expected DNA fragments of about 819 bp in length were amplified using a pair of universal primer corresponding to the RNA polymerase gene and DNA fragments of ca 777 bp and 724 bp in length were amplified using specific primers that have been designed based o...
595.

Race- and Isolate-specific Molecular Marker Development through Genome- Realignment Enables Detection of Korean Plasmodiophora brassicae Isolates, Causal agents of Clubroot Disease

Ji-Yun Jeong, Arif Hasan Khan Robin, Sathishkumar Natarajan, Rawnak Laila, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou 한국식물병리학회 The Plant Pathology Journal 8 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 6 506-513 (8 pages)

Ji-Yun Jeong, Arif Hasan Khan Robin, Sathishkumar Natarajan, Rawnak Laila, Hoy-Taek Kim, Jong-In Park, Ill-Sup Nou 저
...
- 3,000원
in the laboratory and its races are genetically variable worldwide. In Korea, there are at least five races that belongs to four pathotype groups. A recent study conducted in Korea attempted to develop molecular markers based on ribosomal DNA polymorphism to detect P. brassicae isolates, but none of those markers was either race-specific or pathotype-specific. Our current study aimed to develop race- and isolate-specific markers by exploiting genomic sequence variations. A total of 119 markers...
596.

Loop-mediated Isothermal Amplification assay for Detection of Candidatus Liberibacter Asiaticus, a Causal Agent of Citrus Huanglongbing

Cheol Woo Choi, Jae Wook Hyun, Rok Yeon Hwang, Charles A Powell 한국식물병리학회 The Plant Pathology Journal 7 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 5 499-505 (7 pages)

Cheol Woo Choi, Jae Wook Hyun, Rok Yeon Hwang, Charles A Powell 저
...
- 3,000원
Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting...
597.

Identification and Characterization of Colletotrichum Species Associated with Bitter Rot Disease of Apple in South Korea

May Moe Oo, Ha-Yeon Yoon, Hyun A Jang, Sang-Keun Oh 한국식물병리학회 The Plant Pathology Journal 10 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 3 480-489 (10 pages)

May Moe Oo, Ha-Yeon Yoon, Hyun A Jang, Sang-Keun Oh 저
...
- 3,000원
Bitter rot caused by Colletotrichum species is a common fruit rotting disease of apple and one of the economically important disease in worldwide. In 2015 and 2016, distinct symptoms of bitter rot disease were observed in apple orchards in five regions of South Korea. In the present study, infected apples from these regions were utilized to obtain eighteen isolates of Colletotrichum spp. These isolates were identified and characterized according to their morphological characteristics and...
598.

The Roles of Two hfq Genes in the Virulence and Stress Resistance of Burkholderia glumae

Jieun Kim, Mohamed Mannaa, Namgyu Kim, Chaeyeong Lee, Juyun Kim, Jungwook Park, Hyun-Hee Lee, Young-Su Seo 한국식물병리학회 The Plant Pathology Journal 14 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 5호 7 412-425 (14 pages)

Jieun Kim, Mohamed Mannaa, Namgyu Kim, Chaeyeong Lee, Juyun Kim, Jungwook Park, Hyun-Hee Lee, Young-Su Seo 저
...
- 4,300원
The Hfq protein is a global small RNA chaperone that interacts with regulatory bacterial small RNAs (sRNA) and plays a role in the post-transcriptional regulation of gene expression. The roles of Hfq in the virulence and pathogenicity of several infectious bacteria have been reported. This study was conducted to elucidate the functions of two hfq genes in Burkholderia glumae, a causal agent of rice grain rot. Therefore, mutant strains of the rice-pathogenic B. glumae BGR1, targeting each of the...
599.

Comparative Pathogenicity of Fusarium graminearum Isolates from Wheat Kernels in Korea

Sanghyun Shin, Jae-Han Son, Jong-Chul Park, Kyeong-Hoon Kim, Young-mi Yoon, Young-Keun Cheong, Kyong-Ho Kim, Jong-Nae Hyun, Chul Soo Par 한국식물병리학회 The Plant Pathology Journal 9 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 5호 1 347-355 (9 pages)

Sanghyun Shin, Jae-Han Son, Jong-Chul Park, Kyeong-Hoon Kim, Young-mi Yoon, Young-Keun Cheong, Kyong-Ho Kim, Jong-Nae Hyun, Chul Soo Par 저
...
- 3,000원
Fusarium head blight (FHB) caused by Fusarium species is a major disease of wheat and barley around the world. FHB causes yield reductions and contamination of grains with trichothecene mycotoxins including; nivalenol (NIV), deoxynivalenol (DON), 3-cetyldeoxynivalenol (3-ADON), and 15-acetyldeoxynivalenol (15-ADON). The objectives of this study were to identify strains of F. graminearum isolated in Korea from 2012-harvested wheat grain and to test the pathogenicity of these NIV- and...
600.

Morphological and Molecular Characterization of Heterodera schachtii and the Newly Recorded Cyst Nematode, H. trifolii Associated with Chinese Cabbage in Korea

Abraham Okki Mwamula, Hyoung-Rai Ko, Youngjoon Kim, Young Ho Kim, Jae-Kook Lee, Dong Woon Lee 한국식물병리학회 The Plant Pathology Journal 11 Pages

한국식물병리학회 The Plant Pathology Journal 2018, 34권 4호 4 297-307 (11 pages)

Abraham Okki Mwamula, Hyoung-Rai Ko, Youngjoon Kim, Young Ho Kim, Jae-Kook Lee, Dong Woon Lee 저
...
- 4,000원
essential in differentiating the two nematode species. A comparison of morphometric measurements of both infective juveniles and vulval cones of cysts showed significant differences between the two cyst nematodes. When total RNA and genomic DNA were extracted from a mixed field population, COI genes and ITS regions were clearly amplified with primers of the two Heterodera species, suggesting that Heterodera population collected from the Chinese cabbage field consisted of a mixture of two...

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