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661.

Development of a universal plate-agglutination test for detecting Haemophilus parasuis

Dingqian Guo, Cheng Tang, Quan Hai, Guoqing Shao, Hua Yue* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 13 355-357 (3 pages)

Dingqian Guo, Cheng Tang, Quan Hai, Guoqing Shao, Hua Yue* 저
...
- 1,500원
Due to the serovar diversity in Haemophilus (H.) parasuis, it is difficult to develop a universal serological method for detection of this pathogen. Here, we report a universal plate-agglutination test for detecting H. parasuis. Diagnostic antisera were prepared by mixing antisera of serovars 4, 5, 12, 13 and 14 in the optimized ratio. The results of the plate-agglutination test showed that the diagnostic antisera could agglutinate with all 15 reference strains of H. parasuis and 74/75 clinical...
662.

Optimization of culture media of pathogenic Mycoplasma hyopneumoniae by a response surface methodology

Mi-Hyun Hwang, Dereje Damte, Min-Hee Cho, Young-Hoan Kim, Seung-Chun Park* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 8 327-332 (6 pages)

Mi-Hyun Hwang, Dereje Damte, Min-Hee Cho, Young-Hoan Kim, Seung-Chun Park* 저
...
- 3,000원
Composition of culture medium for mass production of Mycoplasma hyopneumoniae was optimized using a response surface methodology (RSM). Initially, the influence of glucose, thallium acetate, fresh yeast extract, horse serum, and porcine serum on the production of mycoplasmal protein was assessed using a 'one factor at a time' technique. Next, factors with a significant effect, including fresh yeast extract, and horse and porcine sera, were selected for further optimization using a central...
663.

Detection of Escherichia coli O157 and Escherichia coli O157:H7 by the immunomagnetic separation technique and stx1 and stx2 genes by multiplex PCR in slaughtered cattle in Samsun Province, Turkey

G?khan Inat*, Belgin Siriken 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 7 321-326 (6 pages)

G?khan Inat*, Belgin Siriken 저
...
- 3,000원
This study was conducted to investigate the presence of Escherichia (E.) coli O157 and E. coli O157:H7 and stx1 and stx2 genes on cattle carcasses and in rectal samples collected from Samsun Province of Turkey. A total of 200 samples collected from cattle carcasses and the rectal contents of 100 slaughtered cattle from two commercial abattoirs were tested using the immunomagnetic separation technique and multiplex PCR methods. E. coli O157 and E. coli O157:H7 were detected in 52 of the 200...
664.

Simultaneous detection and subtyping of porcine endogenous retroviruses proviral DNA using the dual priming oligonucleotide system

Hyoung-Joon Moon, Seong-Jun Park, Hye-Kwon Kim, Soo-Kyung Ann, Semi Rho, Hyun-Ok Keum, Bong-Kyun Park* 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 14 269-271 (3 pages)

Hyoung-Joon Moon, Seong-Jun Park, Hye-Kwon Kim, Soo-Kyung Ann, Semi Rho, Hyun-Ok Keum, Bong-Kyun Park* 저
...
- 1,500원
The purpose of this study was to develop a multiplex PCR that can detect porcine endogenous retrovirus (PERV) proviral genes (pol, envA, envB, envC) and porcine mitochondrial DNA, using a dual priming oligonucleotide (DPO) system. The primer specifically detected the PERV proviral genes pol, envA, envB, envC, and porcine mitochondrial DNA only in samples of pig origin. The sensitivity of the primer was demonstrated by simultaneous amplification of all 5 target genes in as little as 10 pg of pig...
665.

Identification of Arcanobacterium pyogenes isolated by post mortem examinations of a bearded dragon and a gecko by phenotypic and genotypic properties

H. ?lbegi-Mohyla, M. Hijazin, J. Alber, C. L?mmler, A. A. Hassan, A. Abdulmawjood, E. Prenger-Berninghoff, R. Weiß, M. Zsch?ck 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 13 265-267 (3 pages)

H. ?lbegi-Mohyla, M. Hijazin, J. Alber, C. L?mmler, A. A. Hassan, A. Abdulmawjood, E. Prenger-Berninghoff, R. Weiß, M. Zsch?ck 저
...
- 1,500원
The present study was designed to identify phenotypically and genotypically two Arcanobacterium (A.) pyogenes strains isolated by post mortem examinations of a bearded dragon and a gecko. The A. pyogenes strains showed the typical biochemical properties and displayed CAMP-like synergistic hemolytic activities with various indicator strains. The species identity could be confirmed genotypically by amplification and sequencing of the 16S rDNA gene and, as novel target gene, by sequencing of the...
666.

Development and evaluation of a multiplex PCR assay for simultaneous detection of Flavobacterium psychrophilum, Yersinia ruckeri and Aeromonas salmonicida subsp. salmonicida in culture fisheries

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 9 235-241 (7 pages)

Ertan Emek Onuk, Alper Ciftci*, Arzu Findik, Yuksel Durmaz 저
...
- 3,000원
Bacterial cold water disease, enteric red mouth disease and frunculosis are the common bacterial diseases of fish worldwide. The etiologic agents of these diseases are Flavobacterium (F.) psychrophilum, Yersinia (Y.) ruckeri and Aeromonas (A.) salmonicida subsp. salmonicida, respectively. In this study, a multiplex polymerase chain reaction (m-PCR) method with YER8/10-Fer3/4-FP1/3 primer pairs which can identify these fish pathogens simultaneously was developed and optimized. In optimized...
667.

Detection of Helicobacter spp. in gastric, fecal and saliva samples from swine affected by gastric ulceration

Patrizia Casagrande Proietti*, Annalisa Bietta, Chiara Brachelente, Elvio Lepri, Irit Davidson, Maria Pia Franciosini 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 7 221-225 (5 pages)

Patrizia Casagrande Proietti*, Annalisa Bietta, Chiara Brachelente, Elvio Lepri, Irit Davidson, Maria Pia Franciosini 저
...
- 1,500원
The aim of this study was to evaluate the presence of Helicobacter (H.) spp. in swine affected by gastric ulceration. Stomachs from 400 regularly slaughtered swine were subjected to gross pathological examination to evaluate the presence of gastric ulcers. Sixty-five samples collected from ulcerated pars esophagea and 15 samples from non-ulcerated pyloric portions were submitted to histopathological and molecular analyses, to detect Helicobacter spp., H. suis and H. pylori by PCR. Feces and...
668.

64-Channel multi-detector row CT angiographic evaluation of the micropigs for potential living donor lung transplantation

Woong Yoon, Jung Min Ryu, Min Young Lee, Yong Ju Moon, Sang Hun Lee, Jae Hong Park, Seung Pil Yun, Min Woo Jang, Sung Su Park, H 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 3호 2 185-189 (5 pages)

Woong Yoon, Jung Min Ryu, Min Young Lee, Yong Ju Moon, Sang Hun Lee, Jae Hong Park, Seung Pil Yun, Min Woo Jang, Sung Su Park, H 저
...
- 1,500원
Micropigs are the most likely source animals for xenotransplantation. However, an appropriate method for evaluating the lung of micropigs had not been established. Therefore, this study was performed to evaluate the feasibility of 64-channel multi-detector row computed tomography (MDCT) to measure the diameter of the pulmonary arteries and the lung volume in micropigs. The mean diameters of the trachea, and left and right bronchi were 1.6 ± 0.17, 1.18 ± 0.14, and 1.1 ± 0.11 cm,...
669.

Development and characterization of stable cell lines constitutively expressing the porcine reproductive and respiratory syndrome virus nucleocapsid protein

Mingeun Sagong, Choi-Kyu Park, Seong-Hee Kim, Sung-Up Moon, Seong-Cheol Cho, Changhee Lee 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 13 169-171 (3 pages)

Mingeun Sagong, Choi-Kyu Park, Seong-Hee Kim, Sung-Up Moon, Seong-Cheol Cho, Changhee Lee 저
...
- 1,500원
industry worldwide. In this study, we attempted to generate and characterize a panel of stable BHK cell lines that constitutively express the nucleocapsid (N) protein of type 1 or type 2 PRRSV. The established BHK cell lines were found to react well with N-specific antibodies as well as the hyperimmune serum of pigs raised against each genotype of PRRSV. Taken together, the data implicate a potential usefulness for the newly generated stable cell lines as a diagnostic reagent for PRRSV serology.
670.

Spectral Doppler ultrasound in the major arteries of normal conscious immature micropigs

Joohyun Jung, Jinhwa Chang, Sunkyoung Oh, Mincheol Choi 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 2호 10 155-159 (5 pages)

Joohyun Jung, Jinhwa Chang, Sunkyoung Oh, Mincheol Choi 저
...
- 1,500원
Spectral waveform analysis of blood flow velocity in the major arteries of six healthy, conscious immature micropigs was determined using Doppler ultrasonography. Doppler spectral tracings were recorded from the external iliac artery, femoral artery, and renal arcuate artery. Tracings were also taken from three parts of the common carotid artery and two parts of the abdominal aorta. Spectral Doppler parameters included peak systolic velocity, early diastolic velocity, peak systolic...

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