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Prevention of Diabetes Using Adenoviral Mediated Hepatocyte Growth Factor Gene Transfer in Mice
Hye-JeongLee, Hyun-JeongKim, Mee-SookRoh, Jae-IkLee, Sung-WonLee, Dong-SikJung, Duk-KyuKim, Mi-KyoungPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.5 3 261-266 (6 pages)
Type 1 diabetes is an organ-specific autoimmune disease caused by the cytotoxic T cells-mediated destruction of the insulin-producing beta cells in the Langerhans pancreatic islets. Hepatocyte growth factor (HGF) is a potent mitogen and a promoter of proliferation of insulin producing beta cells of pancreatic islets. To study the role of HGF via viral vector in the development of streptozotocin (STZ)-induced diabetes in mice, we have developed an adenoviral vector genetically engineered to carry... -
Roles of Gonadal Steroids on Exocrine Secretion of Isolated Perfused Rat Pancreas
HyungSeoPark, SeHoonKim, HyoungJinPark, MeeYoungLee, YoungHeeHan 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.4 5 217-221 (5 pages)
To clarify the roles of gonadal steroids on pancreatic exocrine secretion, effects of progesterone and estradiol-17β on spontaneous and secretagogue-induced exocrine response of isolated perfused rat pancreas were investigated. Intra-arterial infusion of progesterone resulted in significant increase of the spontaneous pancreatic fluid and amylase secretion dose-dependently. However, estradiol-17β did not exert any influence on spontaneous pancreatic exocrine secretion. Exogenous secretin,... -
Comparative Analysis of Phospholipase D2 Localization in the Pancreatic Islet of Rat and Guinea Pig
GyeongRyulRyu, Myung-JunKim, Chan-HeeSong, DoSikMin, Duck-JooRhie, Shin-HeeYoon, SangJuneHahn, Myung-SukKim, Yang-HyeokJo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.4 4 211-215 (5 pages)
of phospholipase D2 (PLD2) in the pancreatic islet (the islet of Langerhans) depending on species, we conducted a morphological experiment in the rat and guinea pig. Since individual islets display a typical topography with a central core of B cell mass and a peripheral boundary of A, D, and PP cells, double immunofluorescent staining with a panel of antibodies was performed to identify PLD2-immunoreactive cells in the islets PLD2 immunoreactivity was mainly present in A and PP cells of the rat... -
Altered Expression of Aquaporins in Rat Submandibular Glands after Parasympathetic Denervation
JiYeonJung, KangOkByun, WonJaeKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 5 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2003, Vol.7 No.2 8 97-101 (5 pages)
weight loss and morphologic changes, including cell loss and atrophy, as the time elapsed after parasympathetic denervation increased, whereas there were no histologic alteration in control side. AQP5 which is known to reside in apical membrane and secretory caraliculi of the submandibular acini were gradually underexpressed according, as the time after denervation increased. Expression of AQP4 in submandibular ductal epithelium was down-regulated after denervation. Besides, AQP3 and 8, which is... -
Altered Secretory Pattern of Pancreatic Enzymes and Gastrointestinal Hormones in Streptozotocin-induced Diabetic Rats
Myung-JunKim, GyeongRyulRyu, Sae-YoungYi, DoSikMin, Duck-JooRhie, ShinHeeYoon, SangJuneHahn, Myung-SukKim, Yang-HyeokJo 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.6 6 311-317 (7 pages)
This study was performed to investigate the pancreatic exocrine dysfunction in streptozotocin- induced diabetic rats. Changes in pancreatic enzymes secretion and in pancreatic enzymes content were observed. The output and the tissue content of amylase were significantly reduced in diabetic rats, while the output and the content of lipase were increased. Plasma secretin and cholecystokinin (CCK) concentrations of diabetic rats were significantly increased compared to those of normal rats. The... -
Molecular Mechanism of Pancreatic Bicarbonate Secretion
MinGooLee, JeWooKim, KyungHwanKim, ShmuelMuallem 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2002, Vol.6 No.3 1 131-138 (8 pages)
Thanks to recent progress in availability of molecular and functional techniques it became possible to search for the basic molecular and cellular processes that mediate and control HCO3 and fluid secretion by the pancreatic duct. The coordinated action of various transporters on the luminal and basolateral membranes of polarized epithelial cells mediates the transepithelial HCO3 transport, which involves HCO3 absorption in the resting state and HCO3 secretion in the... -
Effects of Pretreatment of Serotonin Synthesis Inhibitor p-chlorophenylalanine on Lipopolysaccharide-induced Anorexia in Rats
So-youngPark, ByungSuckKim, SeoungSookBack 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2001, Vol.5 No.2 3 133-138 (6 pages)
In the present study, we investigated the effect of pretreatment of p-chlorophenylalanine (PCPA), inhibitor of serotonin synthesis, on lipopolysaccharide (LPS)-induced anorexia in rats. First of all, effects of PCPA injection on food intake and body weight in rats were investigated. During 4 days of PCPA injection (300 mg/kg BW once a day), food intake was decreased by 33% and daily gain in body weight was inhibited compared with controls. Twenty-four hours after last PCPA injection, food... -
Regulation of the Contraction Induced by Emptying of Intracellular Ca2+ Stores in Cat Gastric Smooth Muscle
Hye-JungBaek, Sang-SooSim, Duck-JooRhie, ShinHeeYoon, SangJuneHahn, Yang-HyeokJo, Myung-SukKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2000, Vol.4 No.2 5 113-120 (8 pages)
To investigate the mechanism of smooth muscle contraction induced by emptying of intracellular Ca2 stores, we measured isometric contraction and 45Ca2 influx. CaCl2 increased Ca2 store emptying- induced contraction in dose-dependent manner, but phospholipase C activity was not affected by the Ca2 store emptying-induced contraction. The contraction was inhibited by voltage-dependent Ca2 channel antagonists dose dependently, but not by TMB-8 (intracellular... -
Regulation of Magnesium Release by cAMP during Chemical Hypoxia in the Rat Heart and Isolated Ventricular Myocytes
JinShangKimAntonioScarpa 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 10 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.1 8 59-68 (10 pages)
Chemically induced hypoxia has been shown to induce a depletion of ATP. Since intracellular free Mg2 ([Mg2]i) appears to be tightly regulated following cellular energy depletion, we hypothesized that the increase in [Mg2]i would result in Mg2 extrusion following hormonal stimulation. To determine the relation between Mg2 efflux and cellular energy state in a hypoxic rat heart and isolated myocytes, [Mg2]i, ATP and Mg2 content were measured by... -
Effects of Small Molecular Antioxidants on Cerulein-induced Acute Pancreatitis in Rat
JooYoungChoiKyungHwanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.5 11 629-635 (7 pages)
It has been suggested that oxygen free radicals are involved in the initiation process of acute pancreatitis, although its pathogenesis is not clear. This study evaluates the roles of oxygen radicals and the effects of small molecular antioxidants (rebamipide, N-acetyl-cysteine, allopurinol, -carotene) on the development of cerulein-induced acute pancreatitis. Acute edematous pancreatitis was induced by the intravenous infusion of cerulein at supramaximal dose of 10 ㄍg/kg/hour...
