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In vitro treatment of lipopolysaccharide increases invasion of Pasteurella multocida serotype B:2 into bovine aortic endothelial cells
Seng Kar Yap, Zunita Zakaria, Siti Sarah Othman, Abdul Rahman Omar 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2018, 제 19권 제 2호 7 207-215 (9 pages)
bacterium when invading the bloodstream is unclear. This study aimed to characterize the effects of immunomodulatory molecules, namely dexamethasone and lipopolysaccharide, on the invasion efficiency of P. multocida serotype B:2 toward bovine aortic endothelial cells (BAECs) and the involvement of actin microfilaments in the invasion mechanism. The results imply that treatment of BAECs with lipopolysaccharide at 100 ng/mL for 24 h significantly increases the intracellular bacteria number per cel... -
Establishment and evaluation of a murine ανβ3-integrin- expressing cell line with increased susceptibility to Foot- and-mouth disease virus
Wei Zhang, Kaiqi Lian, Fan Yang, Yang Yang, Zhijian Zhu, Zixiang Zhu, Weijun Cao, Ruoqing Mao, Ye Jin, Jijun He, Jianhong Guo, X 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 3 265-272 (8 pages)
a stable CHO-677 cell line expressing the murine ανβ3 heterodimer (designated as “CHO-677-mανβ3” cells) was established using a highly efficient lentiviral-mediated gene transfer technique. Integrin subunits αν and β3 were detected at the gene and protein levels by polymerase chain reaction (PCR) and indirect immunofluorescent assay (IFA), respectively, in the CHO-677-mανβ3 cell line at the 20th passage, implying that these genes were successfully introduced into the CHO-677 cells... -
The potential role of COX-2 in cancer stem cell-mediated canine mammary tumor initiation: an immunohistochemical study
Jian Huang, Di Zhang, Fuqiang Xie, Degui Lin 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 2호 13 225-231 (7 pages)
Increasing evidence suggests that cancer stem cells (CSCs) are responsible for tumor initiation and maintenance. Additionally, it is becoming apparent that cyclooxygenase (COX) signaling is associated with canine mammary tumor development. The goals of the present study were to investigate COX-2 expression patterns and their effect on CSC-mediated tumor initiation in primary canine mammary tissues and tumorsphere models using immunohistochemistry. Patterns of COX-2, CD44, octamer-binding... -
Radiation up-regulates the expression of VEGF in a canine oral melanoma cell line
Irene Flickinger*, Barbara C Rütgen, Wilhelm Gerner, Ivana Calice, Alexander Tichy, Armin Saalmüller, Miriam Kleiter 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 13 207-214 (8 pages)
To evaluate radiosensitivity and the effects of radiation on the expression of vascular endothelial growth factor (VEGF) and VEGF receptors in the canine oral melanoma cell line, TLM 1, cells were irradiated with doses of 0, 2, 4, 6, 8 and 10 Gray (Gy). Survival rates were then determined by a MTT assay, while vascular endothelial growth factor receptor (VEGFR)-1 and -2 expression was measured by flow cytometry and apoptotic cell death rates were investigated using an Annexin assay.... -
Suitability of autologous serum for expanding rabbit adipose-derived stem cell populations
Jae-Jun Bahn, Jin-Young Chung, Wooseok Im, Manho Kim, Sae Hoon Kim* 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 11 413-417 (5 pages)
and characterization of rabbit ASCs grown in medium containing autologous serum (AS) and compared these cells to ones cultured with FBS. Morphological changes were monitored by microscopy and flow cytometry. Proliferation rates were assessed with cell counting and ASC phenotypes were characterized by flow cytometry using representative surface markers (CD44 and CD45). Expression of epidermal growth factor, brain-derived neurotrophic factor, and vascular endothelial growth factor was measured by... -
Aerosol delivery of kinase-deficient Akt1 attenuates Clara cell injury induced by naphthalene in the lungs of dual luciferase mice
Arash Minai-Tehrani, Young-Chan Park, Soon-Kyung Hwang, Jung-Taek Kwon, Seung-Hee Chang, Sung-Jin Park, Kyeong-Nam Yu, Ji-Eun Ki 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 2 309-317 (9 pages)
KD did not. Our results showed that naphthalene affected extracellular signal-regulated kinase (ERK) protein levels, ERK-related signaling, and induced Clara cell injury. However, Clara cell injury induced by naphthalene was considerably attenuated in mice exposed to Akt1 KD. Furthermore, a dual luciferase activity assay showed that aerosol delivery of Akt1 WT and naphthalene treatment enhanced cap-dependent protein translation, while reduced cap-dependent protein translation was observed after... -
Radioprotective effects of fucoidan on bone marrow cells: improvement of the cell survival and immunoreactivity
Yun-Young Byon, Mi-Hyoung Kim, Eun-Sook Yoo, Kyu-Kye Hwang, Youngheun Jee, Taekyun Shin, Hong-Gu Joo* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 4호 4 359-366 (8 pages)
we assayed cell viability and immune responses. In a viability assay, fucoidan significantly increased the viability of BMCs. Based on the results of flow cytometric analysis, the increased viability of fucoidan-treated BMCs was attributed to the inhibition of radiation-induced apoptosis. Furthermore, fucoidan altered the production of immune-related cytokines from BMCs and increased the capability of BMCs to induce proliferation of allogeneic splenocytes. Taken together, our study demonstrated... -
Analysis of in vitro apoptosis induced by virulent Korean isolate of classical swine fever virus in peripheral blood B cell line
Seon Mi Kim , Seong In Lim , Jae Young Song , Bang Hun Hyun , 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 4 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2012, 제 52권 제 4호 6 259-262 (4 pages)
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Safety and outcomes of subconjunctival allogenic mesenchymal stem cell transplantation in canine experimental corneal defects
Ju Won Kim , So Young Lee , Hee Myung Park 대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 6 Pages
대한수의학회 Korean Journal of Veterinary Research(구 대한수의학회지) 2012, 제 52권 제 3호 2 157-162 (6 pages)
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EFFECTS OF NITRIC OXIDE SYNTHASE INHIBITORS ON OSTEOCLAST-LIKE CELL FORMATION
Seung-Kyu Ahn, Jung-Kun Kim, Kyung-Suk Cha 대한치과교정학회 The Korean Journal of Orthodontics 8 Pages
대한치과교정학회 The Korean Journal of Orthodontics 1995, 제 25권 제 6호 7 715-722 (8 pages)
the cell level. Convincing evidence has now been provided to support the view that osteoclasts are derived from mononuclear cells that originate in the bone marrow or other hematopoietic organs and they migrate to the bones via vascular routes. Nitric oxide(NO), which accounts for the biological properties of endothelium-derived relaxmg factor(EDRF), is the endogenous stimulator of soluble guanylate cylase. The discovery of the formation of nitric oxide(NO) from L-arginine in mammalian tissues...


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