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Opening of ATP-sensitive K+ Channel by Pinacidil Requires Serine/ Threonine Phosphorylation in Rat Ventricular Myocytes
Yong-GeunKwakSoo-WanChae 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 11 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1999, Vol.3 No.3 7 293-303 (11 pages)
K (KATP) channel-opening effect of pinacidil were investigated in single rat ventricular myocytes using patch clamp technique. In cell-attached patches, pinacidil (100 μM) induced the opening of the KATP channel, which was blocked by the pretreatment with H-7 (100 μM) whereas enhanced by the pretreatment with genistein (30 μM) or tyrphostin A23 (10 μM). In inside-out patches, pinacidil (10 μM) activated the KATP channels in the presence of ATP (0.3 mM) or AMP-PNP (0.3 mM) and in a... -
Effects of Prostaglandin E2 on the Spontaneous Contractions and Electrical Activities of the Antral Circular Muscle in Guinea-pig Stomach
JongYoonKim, SungJoonKim, TongMookKang, SangJinLee, JaeYeoulJun, InsukSo, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1998, Vol.2 No.3 11 361-368 (8 pages)
an intracellular recording technique. To elucidate the underlying mechanism for its effect on contractility, ionic currents were also measured using a whole-cell patch clamp method. The basal tone by PGE2 was variable, whereas the magnitude of phasic contractions was reduced (19.0⁑2.1%, n=19). The resting membrane potentials were hyperpolarized (4.4⁑0.5 mV, n=10), and plateau potentials were lowered (2.9⁑0.5 mV, n=10). In most cases, however, the initial peak... -
Tetanus-induced LTD of Developing MNTB-LSO Synapses in Rat is Dependent on Postsynaptic Ca2+
SeungCheolAhn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2007, Vol.11 No.3 1 79-84 (6 pages)
synapses is most active during the first postnatal week and the long term depression (LTD) has been suggested as one of its mechanisms, LTD of MNTB-LSO synapses was investigated in neonatal rat brain stem slices with the whole cell voltage clamp technique. In Mg2 free condition, tetanus (10 stimuli at 10 Hz for 2 min) in the current clamp mode induced a robust LTD of isolated D, L-APV-sensitive postsynaptic currents (PSCs) for more than 30 min (n=6, 2.4⁑0.4% of the control), while... -
Methylene Blue-stained Interstitial Cells are Electrically Active in the Myenteric Board Freshly Prepared from the Murine Small Intestine
KyuPilLee, Ju-HongJeon, InsukSo, KiWhanKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2006, Vol.10 No.4 5 193-198 (6 pages)
technique to freshly isolate the cells is indispensable to explore the electrophysiological properties of the ICCs. To apply an enzyme solution on the serosal surface for cell isolation, the intestine was inverted and 0.02% trypsin solution and 0.04% collagenase solution were applied to serosal cavity. After the enzyme treatment, mucosal layer was removed and longitudinal muscle layer was gently separated from the rest of tissue. The thin layer was stretched in the recording chamber and mounted... -
Ca2+-activated K+ Currents of Pancreatic Duct Cells in Guinea-pig
Han-WookLee, JingchaoLi, Na-YounKoo, ZhengGenPiao, SungMinHwang, Jae-WoongHan, Han-SaemChoi, Jong-HeunLee, JoongSooKim, KyungpyoPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.6 7 335-338 (4 pages)
transepithelial K transport of normal duct cells in exocrine glands are scarce. In the present study, we examined the characteristics of K currents in single duct cells isolated from guinea pig pancreas, using a whole-cell patch clamp technique. Both Cl and K conductance were found with KCl rich pipette solutions. When the bath solution was changed to low Cl, reversal potentials shifted to the negative side, 75⁑4 mV, suggesting that this current is... -
Effect of pH on the ATP-sensitive K+ Channel in Aortic Smooth Muscle Cells from Rats
SeHoonKim, IlSuKim, HoeSukKim, ByeongHwaJeon, SeokJongChang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 1997, Vol.1 No.5 11 555-563 (9 pages)
The effects of pH on K currents were investigated in single smooth muscle cells isolated from the thoracic aorta of Wistar-Kyoto rats. Whole-cell K currents were recorded in the conventional configuration of the voltage-clamp technique. Pinacidil (10uM) activated the whole-cell current and the pinacidil-activated current was completely inhibited by glibenclamide (10uM) , an inhibitor of ATP-sensitive K channel (KATP channel). Pinacidil-activated current was reversed at near... -
Voltage-Dependent Ionic Currents and Their Regulation by GTP and Phorbol Ester in the Unfertilized Eggs of Mouse and Hamster
Kim, Ik-Hyun, Kim, Yang-Mi, Haan, Jae-Hee, Park, Choon-Ok, Hong, Seong-Geun 대한생리학회 대한생리학회지 14 Pages
대한생리학회 대한생리학회지 1993, 제 27권 제 1호 9 93-106 (14 pages)
The present study was performed to investigate the properties of ionic currents elicited by voltage pulses in the unfertilized eggs of mouse and hamster by using the whole cell voltage clamp techniques and to find out if there are any differences in properties between eggs of the two rodents. In addition, the modulatory effect of G proteins and protein kinase C (PKC) on the ionic channels were observed. The inward current in hamster eggs was shown to be due to Ca2+ current (ica)). The current... -
Diclofenac, a Non-steroidal Anti-inflammatory Drug, Inhibits L-type Ca2＋ Channels in Neonatal Rat Ventricular Cardiomyocytes
OlegV.Yarishkin, EunMiHwang, DonggyuKim, JaeChealYoo, SangSooKang, DeokRyoungKim, Jae-Hee-JungShin, Hye-JooChung, Ho-SangJeong, DawonKang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.6 5 437-442 (6 pages)
the L-type calcium channels which are essential to the E-C coupling at the level of single ventricular myocytes isolated from neonatal rat heart, using the whole-cell voltage-clamp technique. Only diclofenac of three NSAIDs, including naproxen and ibuprofen, significantly reduced inward whole cell currents. At concentrations higher than 3ՌM, diclofenac inhibited reversibly the Na＋ current and did irreversibly the L-type Ca2＋ channels-mediated inward current (IC50=12.89±0.43ՌM) in... -
Effects of Apigenin on Glutamate-induced [Ca2+]i Increases in Cultured Rat Hippocampal Neurons
Ji-HwaHan, KiJungKim, Hyun-JongJang, JuHoJang, Myung-JunKim, KiWugSung, Duck-JooRhie, Yang-HyeokJo, SangJuneHahn, Mun-YongLee, ShinHeeYoon 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2008, Vol.12 No.2 2 43-50 (8 pages)
Flavonoids have been shown to affect calcium signaling in neurons. However, there are no reports on the effect of apigenin on glutamate-induced calcium signaling in neurons. We investigated whether apigenin affects glutamate-induced increase of free intracellular Ca2+ concentration ([Ca2+]i) in cultured rat hippocampal neurons, using fura-2-based digital calcium imaging and microfluorimetry. The hippocampal neurons were used between 10 and 13 days in culture from embryonic day 18 rats.... -
Modulation of Large Conductance Ca2+-activated K+ Channel of Skin Fibroblast (CRL-1474) by Cyclic Nucleotides
JihyunYun, SeungtaeKim, HyoweonBang 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 4 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2005, Vol.9 No.2 9 131-134 (4 pages)
Potassium channels in human skin fibroblast have been studied as a possible site of Alzheimer disease pathogenesis. Fibroblasts in Alzheimer disease show alterations in signal transduction pathway such as changes in Ca2 homeostasis and/or Ca2-activated kinases, phosphatidylinositol cascade, protein kinase C activity, cAMP levels and absence of specific K channel. However, little is known so far about electrophysiological and pharmacological characteristics of...
