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Genetic analysis of ORF5 of recent Korean porcine reproductive and respiratory syndrome viruses (PRRSVs) in viremic sera collected from MLV-vaccinating or non-vaccinating farms
Hye Kwon Kim, Jeong Sun Yang, Hyoung Joon Moon, Seong Jun Park, Yuzi Luo, Chul Seung Lee, Dae Sub Song, Bo Kyu Kang, Soo Kyung A 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 2호 5 121-130 (10 pages)
The 23 open reading frame (ORF) 5 sequences of Korean type II porcine reproductive and respiratory syndrome virus (PRRSV) were collected from viremic sera from the (modified live vaccine) MLV-vaccinating and non-vaccinating farms from 2007 to 2008. The samples were phylogenetically analyzed with previous ORF5 sequences, including type I Korean PRRSV, and previously reported or collected sequences from 1997 to 2008. A MN184-like subgroup of type II Korean PRRSV was newly identified in the viremic... -
A biosensor assay for the detection of Mycobacterium avium subsp. paratuberculosis in fecal samples
Vijayarani Kumanan, Sam R. Nugen, Antje J. Baeumner, Yung-Fu Chang* 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 6 35-42 (8 pages)
A simple, membrane-strip-based lateral-flow (LF) biosensor assay and a high-throughput microtiter plate assay have been combined with a reverse transcriptase polymerase chain reaction (RT-PCR) for the detection of a small number (ten) of viable Mycobacterium (M.) avium subsp. paratuberculosis (MAP) cells in fecal samples. The assays are based on the identification of the RNA of the IS900 element of MAP. For the assay, RNA was extracted from fecal samples spiked with a known quantity of (10 to... -
Cytokine response in Balb/c mice infected with Francisellatularensis LVS and the Pohang isolate
Eun-Ju Kim, Sang-Hee Park, Young-Sill Choi, Soo-Kyoung Shim, Mi-Yeoun Park, Man Suck Park, Kyu-Jam Hwang 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 3호 12 309-316 (8 pages)
We investigated the immune response induced by the Francisella (F.)tularensis live vaccine strain (LVS) and the Pohang isolate. After the Balb/c mice were infected intradermally (i.d) with 2 × 10 cfu of F. tularensis LVS and Pohang, respectively, their blood and organs were collected at different times; 0, 3, 6, 24, 72, 96, 120 and 168 h after infection. Using these samples, RT-PCR and ELISA analysis were carried out for the comparative study of the cytokines, including TNF-a, INF-g, IL-2,... -
Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea
Seong Joon Joh, *, Chae Ik Shon, Sung Won Kang, Byoung Han Kim, Byung Yul Jeong, Kyung Gi Lee, Jun Hun Kwon, Gang Jun Heo2 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 11 85-90 (6 pages)
The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2,538 bp open reading frame, which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser/Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1... -
The expression of plasmid mediated afimbrial adhesin genes in an avian septicemic Escherichia coli strain
Eliana Guedes Stehling, *, Tatiana Amabile Campos, Marcelo Brocchi, Vasco Ariston de Carvalho Azevedo, Wanderley Dias da Silveira 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 10 75-83 (9 pages)
An Escherichia coli strain (SEPT13) isolated from the liver of a hen presenting clinical signs of septicaemia had a LD50 of 4.0 × 105 CFU/ml in one-day-old chickens, expressed Ia, Ib, E1, E3, K and B colicins and aerobactin. The strain was ampicillin and streptomycin resistant, and found to have fimA, csgA and tsh DNA related sequences; it could adhere to and invade HEp-2 and tracheal epithelial cells, expressed fimbriae (observed by electron microscopy), and had five plasmids of 2.7, 4.7,... -
Phytotherapeutic effects of Echinacea purpurea in gamma-irradiated mice
Amira M. K. Abouelella, Yasser E. Shahein*, Sameh S. Tawfik, Ahmed M. Zahran 대한수의학회 Journal of Veterinary Science 12 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 4호 5 341-352 (12 pages)
Echinacea (E.) purpurea herb is commonly known as the purple coneflower, red sunflower and rudbeckia. In this paper, we report the curative efficacy of an Echinacea extract in ■-irradiated mice. E. purpurea was given to male mice that were divided into five groups (control, treated, irradiated, treated before irradiation & treated after irradiation) at a dose of 30 mg/kg body weight for 2 weeks before and after irradiation with 3 Gy of ■-rays. The results reflected the detrimental... -
PCR-based detection of genes encoding virulence determinants in Staphylococcus aureus from bovine subclinical mastitis cases
Dewanand Rajaram Kalorey, Yuvaraj Shanmugam, Nitin Vasantrao Kurkure, Kapil Kamalakarrao Chousalkar, Sukhadeo Baliram Barbuddhe 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 8 151-154 (4 pages)
The present study was carried out to genotypically characterize Staphylococcus aureus (S. aureus) isolated from bovine mastitis cases. A total of 37 strains of S. aureus were isolated during processing of 552 milk samples from 140 cows. The S. aureus strains were characterized phenotypically, and were further characterized genotypically by polymerase chain reaction using oligonucleotide primers that amplified genes encoding coagulase (coa), clumping factor (clfA), thermonuclease (nuc),... -
Detection of betanodaviruses in apparently healthy aquarium fishes and invertebrates
Dennis Kaw Gomez, Dong Joo Lim, Gun Wook Baeck, Hee Jeong Youn, Nam Shik Shin, Hwa Young Youn, Cheol Yong Hwang, Jun Hong Park, 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 11 369-374 (6 pages)
Betanodaviruses are the causative agents of viral nervous necrosis (VNN) in cultured marine fish. A total of 237 apparently healthy aquarium fish, marine (65 species) and freshwater (12 species) fishes and marine invertebrates (4 species), which were stocked in a commercial aquarium in Seoul, South Korea, were collected from November 2005 to February 2006. The brains of the fish and other tissues of the invertebrates were examined by reverse transcriptasepolymerase chain reaction (RT-PCR) and... -
Anthelmintic efficacy of extracts of Spigelia anthelmia Linn on experimental Nippostrongylus braziliensis in rats
Olorunfemi Cornelius Jegede, Joseph Ologunja Ajanusi, Ambrose. O. Adaudi, Rowland I. S. Agbede 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 4 229-232 (4 pages)
Spigelia anthelmia Linn is used as a herb and is a common annual weed of cultivation in open re-growths, on unused land in towns as well as on road sides. The plant can grow to approximately 30 cm in height. The aim of this study was to screen extracts of Spigelia anthelmia for their anthelmintic activity against an experimental Nippostrongylus braziliensis infection in rats. Acute oral toxicity occurred at a dose of 1,140 mg/kg, while anthelmintic trials against Nippostrongylus braziliensis in... -
Effect of probiotic containing Saccharomyces boulardii on experimental ochratoxicosis in broilers: hematobiochemical studies
S. B. Agawane, P. S. Lonkar 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 4호 12 359-367 (9 pages)
In the present investigation, the toxicopathological effects of ochratoxin A of 0.5 ppm on hematobiochemical parameters of broilers were studied with efficacy of dietary concentration of probiotic containing yeast culture Saccharomyces boulardii of 10 mg/kg of feed. One hundred twenty day old chicks were randomly divided into four groups, thirty chicks each. Groups A and C chicks were offered normal feed and that added with probiotic Saccharomyces boulardii respectively. The birds in group B...


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