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61.

Use of hydrophilic extra-viral domain of canine distemper virus H protein for enzyme-linked immunosorbent assay development

Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 6 503-509 (7 pages)

Ki-hyun Cho, Jeongmi Kim, Hyun-ah Yoo, Dae-hee Kim, Seung-yong Park, Chang-seon Song, In-soo Choi, Joong-bok Lee, 저
...
- 3,000원
Simple methods for measuring the levels of serum antibody against canine distemper virus (CDV) would assist in the effective vaccination of dogs. To develop an enzyme-linked immunosorbent assay (ELISA) specific for CDV, we expressed hydrophilic extra-viral domain (HEVD) protein of the A75/17-CDV H gene in a pET 28a plasmid-based Escherichia (E.) coli vector system. Expression was confirmed by dot and Western blotting. We proposed that detection of E. coli-expressed H protein might be...
62.

Dietary germanium biotite supplementation enhances the induction of antibody responses to foot-and-mouth disease virus vaccine in pigs

Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 15 443-447 (5 pages)

Jin-A Lee, Bock-Gie Jung, Myunghwan Jung, Tae-Hoon Kim, Han Sang Yoo, Bong-Joo Lee* 저
...
- 1,500원
We evaluated the potential ability of germanium biotite (GB) to stimulate the production of antibodies specific for foot-and-mouth disease virus (FMDV). To this aim, we measured the total FMDV-specific antibody responses and IgM production after vaccination against FMD both experimentally and in the field. GB supplementation with FMDV vaccination stimulated the production of anti-FMDV antibodies, and effectively increased IFN-γ and TNF-α levels. These results suggest that GB may be a novel...
63.

A reverse transcription loop-mediated isothermal amplification assay to rapidly diagnose foot-and-mouth disease virus C

Yao-zhong Ding, Jian-hua Zhou, Li-na Ma, Yan-ni Qi, Gang Wei, Jie Zhang, *, Yong-guang Zhang* 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 11 423-426 (4 pages)

Yao-zhong Ding, Jian-hua Zhou, Li-na Ma, Yan-ni Qi, Gang Wei, Jie Zhang, *, Yong-guang Zhang* 저
...
- 1,500원
A reverse transcription loop-mediated isothermal amplification (RT-LAMP) assay was developed to rapidly detect foot-and-mouth disease virus serotype C (FMDV C). By testing 10-fold serial dilutions of FMDV C samples, sensitivity of the FMDV C RT-LAMP was found to be 10 times higher than that of conventional reverse transcription- PCR (RT-PCR). No cross-reactivity with A, Asia 1, or O FMDV or swine vesicular disease virus (SVDV) indicated that FMDV C RT-LAMP may be an exciting novel method for...
64.

Prevalence and characteristics of Shiga toxin-producing Escherichia coli (STEC) from cattle in Korea between 2010 and 2011

Eun Kang, Sun Young Hwang, Ka Hee Kwon, Ki Yeon Kim, Jae Hong Kim, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 5 369-379 (11 pages)

Eun Kang, Sun Young Hwang, Ka Hee Kwon, Ki Yeon Kim, Jae Hong Kim, Yong Ho Park* 저
...
- 4,000원
A total of 156 Shiga-like toxin producing Escherichia coli (STEC) were isolated from fecal samples of Korean native (100/568, 18%) and Holstein dairy cattle (56/524, 11%) in Korea between September 2010 and July 2011. Fifty-two STEC isolates (33%) harbored both of shiga toxin1 (stx1) and shiga toxin2 (stx2) genes encoding enterohemolysin (EhxA) and autoagglutinating adhesion (Saa) were detected by PCR in 83 (53%) and 65 (42%) isolates, respectively. By serotyping, six STEC from native cattle and...
65.

Distribution, quantitative load and characterization of Salmonella associated with swine farms in upper-northern Thailand

Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 20 327-334 (8 pages)

Pakpoom Tadee, Kittipong Kumpapong, Danai Sinthuya, Panuwat Yamsakul, Nipa Chokesajjawatee, Supachai Nuanualsuwan, Suchawan Porn 저
...
- 3,000원
This study was conducted to analyze the prevalence and quantitative loads of Salmonella spp. on pig farms in Chiang Mai, Lamphun, Thailand to assess loading levels before slaughtering. The serotype diversity, antimicrobial-resistance pattern and pulse-field type of Salmonella spp. were also characterized to assess the dynamic propagation of the pathogen. The Salmonella-positive prevalence was 246/805 (30.56%), and the quantitative loads varied from 1.48∼4.04 Log10MPN/g, with a mean ± standard...
66.

Evaluation of endoscopically obtained duodenal biopsy samples from cats and dogs in an adapter-modified Ussing chamber

Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 17 297-307 (11 pages)

Isabelle Ruhnke*, John V. DeBiasio, Jan S. Suchodolski, Shelley Newman, Mark W. Musch, Jörg M. Steiner 저
...
- 4,000원
This study was conducted to evaluate an adapter-modified Ussing chamber for assessment of transport physiology in endoscopically obtained duodenal biopsies from healthy cats and dogs, as well as dogs with chronic enteropathies. 17 duodenal biopsies from five cats and 51 duodenal biopsies from 13 dogs were obtained. Samples were transferred into an adapter-modified Ussing chamber and sequentially exposed to various absorbagogues and secretagogues. Overall, 78.6% of duodenal samples obtained from...
67.

A novel low-cost method for Mycobacterium avium subsp. paratuberculosis DNA extraction from an automated broth culture system for real-time PCR analysis

Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 9 233-239 (7 pages)

Miguel Salgado*, Cristobal Verdugo, Cord Heuer, Pedro Castillo, Patricia Zamorano 저
...
- 3,000원
PCR is a highly accurate technique for confirming the presence of Mycobacterium avium subsp. paratuberculosis (Map) in broth culture. In this study, a simple, efficient, and low-cost method of harvesting DNA from Map cultured in liquid medium was developed. The proposed protocol (Universidad Austral de Chile [UACH]) was evaluated by comparing its performance to that of two traditional techniques (a QIAamp DNA Stool Mini Kit and cethyltrimethylammonium bromide [CTAB] method). The results were...
68.

Development of a real-time SYBR Green PCR assay for the rapid detection of Dermatophilus congolensis

Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 대한수의학회 Journal of Veterinary Science 4 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 17 491-494 (4 pages)

Alfredo García, Remigio Martínez, José Manuel Benitez-Medina, David Risco, Waldo Luis García, Joaqu& 저
...
- 1,500원
Methods such as real time (RT)-PCR have not been developed for the rapid detection and diagnosis of Dermatophilus(D.)congolensis infection. In the present study, a D. congolensis-specific SYBR Green RT-PCR assay was evaluated. The detection limit of the RT-PCR assay was 1 pg of DNA per PCR reaction. No cross-reaction with nucleic acids extracted from Pseudomonas aeruginosa, Mycobacterium tuberculosis, Staphylococcus aureus, or Austwickia chelonae was observed. Finally, the RT-PCR assay was used...
69.

Evaluation of changes in left ventricular myocardial function observed in canine myocardial dysfunction model using a two-dimensional tissue tracking technique

Lina Hamabe, Ryuji Fukushima, Keisuke Kawamura, Yusuke Shinoda, Hsu Huai-Che, Shuji Suzuki, Derya Aytemiz, Toshiroh Iwasaki, Ryo 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 16 355-362 (8 pages)

Lina Hamabe, Ryuji Fukushima, Keisuke Kawamura, Yusuke Shinoda, Hsu Huai-Che, Shuji Suzuki, Derya Aytemiz, Toshiroh Iwasaki, Ryo 저
...
- 3,000원
This study was conducted to assess the ability of two-dimensional tissue tracking (2DTT) to evaluate changes in left ventricular (LV) myocardial function associated with sustained high electrical pacing. Pacemakers were implanted at the right ventricular (RV) apex of five female Beagles, and sustained high electrical pacing of 250 beats per minute (bpm) was performed for three consecutive weeks. Conventional echocardiography and 2DTT were performed at baseline, and at every week for three weeks...
70.

Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus

Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 8 291-297 (7 pages)

Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 저
...
- 3,000원
A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (HI) test for the detection and titration of NDV-specific antibodies present in chicken sera. The rHN antigen produced high HA titers of 2 per 25 μL, which were similar to those of the...

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