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Re-evaluation of Hypocrea pseudogelatinosa and H. pseudostraminea isolated from shiitake mushroom (Lentinula edodes) cultivation in Korea and Japan
한국식물병리학회 The Plant Pathology Journal 16 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 1 341-356 (16 pages)
Shiitake (Lentinula edodes) is the most economically important cultivated mushroom, but yields are impacted by its competitor, Trichoderma spp. We previously found two unidentified Trichoderma species growing in bedlogs and sawdust shiitake media in Korea. Here, we identify and re-describe those two species based on molecular sequence data, morphology, and culture characteristics. Well-supported clades based on phylogenetic analyses of internal transcribed spacer, translation elongation factor... -
Mode of Action of Streptomycin Resistance in the Citrus Canker Pathogen (Xanthomonas smithii subsp. citri) in Jeju Island
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 11 207-211 (5 pages)
It has been known that streptomycin resistance in bacteria can occur as a results of chromosomal mutation or through gene acquisition or both. Chromosomal mutations for resistances are point mutations in the rpsL gene, which alter ribosomal protein S12. Acquired resistance has occurred when an SmR plasmid carrying transposon Tn5393 with tandem strA-strB gene is transferred by conjugation. A total of 686 isolates of Xanthomonas smithii subsp. citri causal agent of citrus canker disease were... -
Migration and Distribution of Graft-inoculated Jujube Witches`-broom Phytoplasma within a Cantharanthus roseus Plant
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 8 191-196 (6 pages)
Periwinkle seedlings (Cantharanthus roseus) were inoculated with jujube witches`- broom (JWB) phytoplasma via grafting to analyze the migration of JWB phytoplasmas within the host plant. The phytoplasmas were detected using nested polymerase chain reaction (PCR) and fluorescence microscopy. Fluorescence microscopy was a simple and easy method of detecting phytoplasmas; however, it was not sufficiently sensitive to detect very low phytoplasma concentrations. Therefore, the migration of JWB... -
Identification of Trichoderma, a Competitor of Shiitake Mushroom (Lentinula edodes), and Competition between Lentinula edodes and Trichoderma species in Korea
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 2호 2 137-148 (12 pages)
of shiitake mushroom competitors, 289 isolates of Trichoderma spp. were collected from shiitake mushroom farms in different districts and the Forest Mushroom Research Center of Korea, among which 29 representative strains were selected. Based on the DNA sequences of the rpb2 and tef1 genes and the ITS rDNA, and their morphological characteristics, they were identified as T. atroviride, T. citrinoviride, T. harzianum, T. longibrachiatum, and two undescribed species, Trichoderma spp. 1 and 2, whic... -
Research Notes : Simple and Rapid Detection of Potato Leafroll virus by Reverse Transcription Loop-mediated Isothermal Amplification
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 13 385-389 (5 pages)
A new reverse transcription loop-mediated isothermal amplification (RT-LAMP) method for the Potato leafroll virus (PLRV) was developed and compared with conventional reverse transcription polymerase chain reaction (RT-PCR) to address its advantages over RTPCR. RT-LAMP primers were designed from the open reading frame 3 (ORF3) sequence of PLRV. The RTLAMP reactions were conducted without or with a set of loop primers. By real-time monitoring using Turbimeter, the RT-LAMP (with loop primers)... -
Research Notes : Characteristics of Cucumber mosaic virus isolated from Zea mays in Korea
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 11 372-377 (6 pages)
A virus causing mottle and stunt symptom on Zea mays was observed around Ulleng-do, Korea and identified as Cucumber mosaic virus (CMV-ZM) based upon biological, serological, and molecular characteristics. In host range studies, the CMV-ZM isolate produced local lesions on Datura stramonium, Vigna unguiculata, Cucurbita moschata, Chenopodium amaranticolor, Ch. quinoa, whereas this isolate produced systemic mosaic on Nicotiana tabacum cv. ``Xanthi-nc``, Capsicum annuum, Solanum lycopersicum,... -
Research Notes : Reclassification of Xanthomonas Isolates Causing Bacterial Leaf Spot of Euphorbia pulcherrima
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 9 360-366 (7 pages)
Bacterial leaf spot of Euphorbia pulcherrima has been reported in many countries. Characterization by polyphasic approaches indicated that the isolates from India, USA and New Zealand could be distinguished based on rep-PCR profiles and gyrB phylogenies, while the Chinese isolates should be ascribed to Xanthomonas axonopodis pv. poinsettiicola. -
Research Articles : Evaluation and Verification of Barley Genotypes with Known Genes for Resistance to Barley yellow mosaic virus and Barley mild mosaic virus Under Field Conditions in South Korea
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 4 324-332 (9 pages)
Soil-borne barley yellow mosaic disease caused by Barley yellow mosaic virus (BaYMV) or Barley mild mosaic virus (BaMMV) gives a serious threat to the winter barley cultivated in the southern regions in Korea. It is important to develop resistant varieties for stable and high-yield production. The objectives of this study were to evaluate 22 genotypes of exotic barley germplasms carrying the resistance genes rym1 through rym12, with the exception of rym10, and to determine the genes that confer... -
Research Articles : Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 1 301-309 (9 pages)
The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination... -
RT-PCR Detection of Five Quarantine Plant RNA Viruses Belonging to Potyand Tospoviruses
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 12 291-296 (6 pages)
In order to detect quarantine plant viruses, we developed reverse transcription-polymerase chain reaction (RT-PCR) primer pairs for five single-stranded (ss) plant RNA viruses that are not currently reported in Korea but could be potential harmful plant viral pathogens. Three viruses such as Chilli veinal mottle virus (ChiVMV), Colombian datura virus (CDV), and Tobacco etch virus (TEV) belong to the genus Potyvirus while Chrysanthemum stem necrosis virus (CSNV) and Iris yellow spot virus (IYSV)...


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