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A LysM Domain-Containing Protein LtLysM1 Is Important for Vegetative Growth and Pathogenesis in Woody Plant Pathogen Lasiodiplodia theobromae
Dulanjalee Lakmali Harishchandra, Wei Zhang, Xinghong Li, Kandawatte Wedaralalage Thilini Chethana, Kevin David Hyde, Siraprapa Brooks, Jiye Yan, Junbo Peng 한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2020, 36권 4호 3 323-334 (12 pages)
Lysin motif (LysM) proteins are reported to be necessary for the virulence and immune response suppression in many herbaceous plant pathogens, while far less is documented in woody plant pathogens. In this study, we preliminarily characterized the molecular function of a LysM protein LtLysM1 in woody plant pathogen Lasiodiplodia theobromae. Transcriptional profiles revealed that LtLysM1 is highly expressed at infectious stages, especially at 36 and 48 hours post inoculation. Amino acid sequence... -
Antibody-Mediated Resistance to Rhizomania Disease in Sugar Beet Hairy Roots
M. Jafarzade, M. Ramezani, F. Hedayati, Z. Mokhtarzade, B. Zare, M. S. Sabet, P. Norouzi, M. A. Malboobi 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 6호 15 692-697 (6 pages)
Agrobacterium rhizogenes–mediated transformation of sugar beet hairy roots expressing single-chain variable fragment (scFv) was exploited to evaluate the efficacy of four antibody-based constructs for interfering with the Beet necrotic yellow vein virus infection. The scFv specific to a major coat protein of virus, p21, was targeted to various cellular compartments including the cytosol (pIC and pICC constructs), apoplast (pIA), and mitochondrion (pIM). After mechanical virus inoculation,... -
An Integrated Modeling Approach for Predicting Potential Epidemics of Bacterial Blossom Blight in Kiwifruit under Climate Change
Kwang-Hyung Kim, Young Jin Koh 한국식물병리학회 The Plant Pathology Journal 14 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 7 459-472 (14 pages)
The increasing variation in climatic conditions under climate change directly influences plant-microbe interactions. To account for as many variables as possible that may play critical roles in such interactions, the use of an integrated modeling approach is necessary. Here, we report for the first time a local impact assessment and adaptation study of future epidemics of kiwifruit bacterial blossom blight (KBB) in Jeonnam province, Korea, using an integrated modeling approach. This study... -
In Vitro and In Vivo Inhibitory Effects of Gaseous Chlorine Dioxide against Fusarium oxysporum f. sp. batatas Isolated from Stored Sweetpotato: Study II
Ye Ji Lee, Jin-Ju Jeong, Hyunjung Jin, Wook Kim, Young Chull Jeun, Gyeong-Dan Yu, Ki Deok Kim 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 5호 5 437-444 (8 pages)
Chlorine dioxide (ClO2) has been widely used as an effective disinfectant to control fungal contamination during postharvest crop storage. In this study, Fusarium oxysporum f. sp. batatas SP-f6 from the black rot symptom of sweetpotato was isolated and identified using phylogenetic analysis of elongation factor 1-α gene; we further examined the in vitro and in vivo inhibitory activities of ClO2 gas against the fungus. In the in vitro medium tests, fungal population was significantly inhibited... -
Fungichromin Production by Streptomyces padanus PMS-702 for Controlling Cucumber Downy Mildew
Ya-Ting Fan, Kuang-Ren Chung, Jenn-Wen Huang 한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 4호 6 341-350 (10 pages)
Streptomyces padanus PMS-702 strain produces a polyene macrolide antibiotic fungichromin and displays antagonistic activities against many phytopathogenic fungi. In the present study, experimental formulations were assessed to improve the production of fungichromin, the efficacy of PMS-702 on the suppression of sporangial germination, and the reduction of cucumber downy mildew caused by Pseudoperonospora cubensis. PMS-702 strain cultured in a soybean meal-glucose (SMG) medium led to low levels... -
Selection of Reference Genes for Real-time Quantitative PCR Normalization in the Process of Gaeumannomyces graminis var. tritici Infecting Wheat
Li-hua Xie, Xin Quan, Jie Zhang, Yan-yan Yang, Run-hong Sun, Ming-cong Xia, Bao-guo Xue, Chao Wu, Xiao-yun Han, Ya-nan Xue, Li-rong Yang 한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2019, 35권 1호 2 11-18 (8 pages)
soil borne pathogenic fungus associated with wheat roots. The accurate quantification of gene expression during the process of infection might be helpful to understand the pathogenic molecular mechanism. However, this method requires suitable reference genes for transcript normalization. In this study, nine candidate reference genes were chosen, and the specificity of the primers were investigated by melting curves of PCR products. The expression stability of these nine candidates was determined... -
Loop-mediated Isothermal Amplification assay for Detection of Candidatus Liberibacter Asiaticus, a Causal Agent of Citrus Huanglongbing
Cheol Woo Choi, Jae Wook Hyun, Rok Yeon Hwang, Charles A Powell 한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 6호 5 499-505 (7 pages)
Huanglongbing (HLB, Citrus greening disease) is one of the most devastating diseases that threaten citrus production worldwide. Although HLB presents systemically, low titer and uneven distribution of these bacteria within infected plants can make reliable detection difficult. It was known loop-mediated isothermal amplification (LAMP) method has the advantages of being highly specific, rapid, efficient, and laborsaving for detection of plant pathogens. We developed a new LAMP method targeting... -
Disinfection by Ozone Microbubbles Can Cause Morphological Change of Fusarium oxysporum f. sp. melonis Spores
Masahiko Tamaki, Fumiyuki Kobayashi, Hiromi Ikeura, Michio Sato 한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2018, 34권 4호 8 335-340 (6 pages)
To investigate the difference in the disinfectant efficiency of ozone microbubbles (O3MB) and ozone millibubbles (O3MMB), the morphological change of the treated Fusarium oxysporum f. sp. melonis spores was observed with scanning and transmission electron microscopies (SEM and TEM). The disinfectant efficiency of O3MB on F. oxysporum f. sp. melonis spores was greater than that of O3MMB. On observation with SEM, it was revealed that morphological change of F. oxysporum f. sp. melonis spores was... -
Control of Basal Stem Rot Disease in Oil Palm by Supplementation of Calcium, Copper, and Salicylic Acid
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 5호 3 396-406 (11 pages)
Continuous supplementation of mineral nutrients and salicylic acid (SA) as foliar application could improve efficacy in controlling basal stem rot (BSR) disease in oil palm seedling. It is revealed from the results that the highest disease severity index (58.3%) was recorded in T8 treatments at 9 months after inoculation. The best disease control was achieved by T7 treatments (calcium/copper/SA [Ca/Cu/SA]) (5.0%) followed by T1 (5.5%), T5 (5.8%), T3 (8.3%), T6 (8.3%), T4 (13.3%), and T2 (15.8%)... -
Epidemiology and Control of Strawberry Bacterial Angular Leaf Spot Disease Caused by Xanthomonas fragariae
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 4호 2 290-299 (10 pages)
infections were detected using X. fragariae specific primers 245A/B and 295A/B, where 300-bp and 615-bp were respectively amplified. During the nursery stage (from May to August), the pathogen was PCR detected only in maternal plants, but not in soil or irrigation water through the nursery stage. During the cultivation period, from September to March, the pathogen was detected in maternal plants, progeny, and soil, but not in water. Additionally, un-infected plants, when planted with infected...


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