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전체 선택해제
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본문검색 결과만 보기
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Development of Recombinant Coat Protein Antibody Based IC-RT-PCR and Comparison of its Sensitivity with Other Immunoassays for the Detection of Papaya Ringspot Virus Isolates from India
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 4 25-31 (7 pages)
Papaya ringspot virus (PRSV) causes the most widespread and devastating disease in papaya. Isolates of PRSV originating from different geographical regions in south India were collected and maintained on natural host papaya. The entire coat protein (CP) gene of Papaya ringspot virus-P biotype (PRSV-P) was amplified by RTPCR. The amplicon was inserted into pGEM-T vector, sequenced and sub cloned into a bacterial expression vector pRSET-A using a directional cloning strategy. The PRSV coat protein... -
A Short-chain Dehydrogenase/reductase Gene is Required for Infection-related Development and Pathogenicity in Magnaporthe oryzae
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2010, 26권 1호 2 8-16 (9 pages)
T-DNA insertional mutants of M. oryzae, and identified ATMT0879A1 as one of the pathogenicity-defective mutants. Molecular analyses and database searches revealed that a single TDNA insertion in ATMT0879A1 resulted in functional interference with an annotated gene, MGG00056, which encodes a short-chain dehydrogenase/reductase (SDR). The mutant and annotated gene were designated as MoSDR1T-DNA and MoSDR1, respectively. Like other SDR family members, MoSDR1 possesses both a cofactor- binding motif... -
Morphological and Molecular Identification of Penicillium islandicum Isolate KU101 from Stored Rice
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 14 469-473 (5 pages)
We have previously obtained a representative isolate KU101 of the predominant Penicillium species from rice under indoor storage conditions. In this study we attempted to characterize isolate KU101 using its morphological and molecular characteristics. When the micro- and macroscopic characteristics of isolate KU101 were compared with the P. islandicum reference isolate KCCM 34763, isolate KU101 was generally identical to those of isolate KCCM 34763, however, isolate KU101 grew faster and... -
A Bacterial Endophyte, Pseudomonas brassicacearum YC5480 Isolated from the Root of Artemisia sp. Producing Antifungal and Phytotoxic Compounds
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 13 461-468 (8 pages)
An endophytic bacterial strain YC5480 producing antifungal and phytotoxic compounds simultaneously was isolated from the surface sterilized root of Artemisia sp. collected at Jinju area, Korea. The bacterial strain was identified as a species of Pseudomonas brassicacearum based on its 16S rRNA gene sequence analysis and physiological and biochemical characteristics. The seed germination and growth of monocot and dicot plants were inhibited by culture filtrate (1/10-strength Tryptic Soy Broth) of... -
Difference of Physiochemical Characteristics Between Citrus Bacterial Canker Pathotypes and Identification of Korean Isolates with Repetitive Sequence PCRs
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 8 423-432 (10 pages)
The difference of carbon source utilization and fatty acid composition between the pathotypes of Xanthomonas strains, which causing citrus bacterial canker was compared, and the physiochemical characteristics were used to analyze relationship of the strains for the first time. The pattern of several carbon sources utilization and fatty acids composition reliably discriminated the pathotypes of Xanthomonas strains. The dendrogram which was constructed by 95 carbon source utilization profiles... -
Purification and Phytotoxicity of Apicidins Produced by the Fusarium semitectum KCTC16676
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 7 417-422 (6 pages)
method for apicidin extraction, and to test phytotoxicity of apicidin and its analogs. We compared sequences of the translation elongation factor 1-alpha (TEF) gene in KCTC16676 with those from isolates representing diverse Fusarium species, which showed that KCTC16676 belongs to the F. semitectum-F. equiseti species complex. To enhance apicidin production, after culturing isolate KCTC16676 on a wheat medium for 3 weeks at 25oC, the culture was extracted with chloroform. Apicidins were purified... -
Gene Expression Profiling in Rice Infected with Rice Blast Fungus using SAGE
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 3 384-391 (8 pages)
Rice blast disease, caused by the pathogenic fungus Magnaporthe grisea, is a serious issue in rice (Oryza sativa L.) growing regions of the world. Transcript profiling in rice inoculated with the fungus has been investigated using the transcriptomics technology, serial analysis of gene expression (SAGE). Short sequence tags containing sufficient information which are ten base-pairs representing the unique transcripts were identified by SAGE technology. We identified a total of 910 tag sequences... -
Transgenic Rice Plants Expressing an Active Tobacco Mitogen-activated Protein Kinase Kinase Induce Multiple Defense Responses
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2008, 24권 4호 2 375-383 (9 pages)
It is well known that NtMEK2, a tobacco MAPK kinase, is the upstream kinase of both salicylic acidinduced protein kinase and wound-induced protein kinase. In addition, expression of NtMEK2DD, a constitutively active mutant of NtMEK2, is known to induce multiple defense responses in tobacco. In this study, transgenic rice plants that contained an active or inactive mutant of NtMEK2 under the control of a steroid inducible promoter were generated and used to determine if a similar MAPK cascade is... -
Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량
이효정, 박기범, 한연수, 정래동 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2021, 27권 3호 5 120-127 (8 pages)
식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작 물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협 이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예 작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되 고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라 서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital... -
Sclerotinia sclerotiorum에 의한 갯기름나물 균핵병 발생
정원권, 임양숙, 김민기, 김종수 한국식물병리학회 식물병연구 5 Pages
한국식물병리학회 식물병연구 2021, 27권 3호 4 115-119 (5 pages)
2020년 경상북도 포항군 연일읍의 갯기름나물 재배 농가포 장에서 잎에 작은 갈색 반점이 형성되고 잎끝부터 갈변되며 고 사하는 증상이 발생하였다. 균총은 흰색에서 연한 회색을 띠 었다. 균핵은 검은색의 구형 또는 타원형이었다. 자낭반은 컵모 양이며, 크기는 0.5‒0.9 cm이었다. 자낭은 원통형이고 크기는 75‒240×5.9‒17.3 μm이었다. 자낭포자는 무색의 타원형이었으며 크기는 8.4‒10.7×4.8‒5.8 μm이었다. Internal transcribed spacer (ITS) 1과 ITS4 영역의 염기서열 분석결과 Sclerotinia...
