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71.

Development of a multiplex loop-mediated isothermal amplification assay to detect shiga toxin-producing Escherichia coli in cattle

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 19 317-325 (9 pages)

Hee-Jin Dong, Ae-Ri Cho, Tae-Wook Hahn, Seongbeom Cho* 저
...
- 3,000원
A multiplex loop-mediated isothermal amplification (mLAMP) assay was developed for simultaneous detection of the stx1 and stx2 genes and applied for detection of shiga toxin-producing Escherichia coli (STEC) in cattle farm samples. Two target genes were distinguished based on Tm values of 85.03 ± 0.54C for stx1 and 87.47 ± 0.35C for stx2. The mLAMP assay was specific (100% inclusivity and exclusivity), sensitive (with a detection limit as low as 10 fg/μL), and quantifiable (R = 0.9313). The...
72.

Extent of Mycobacterium bovis infection in dairy cattle herds subject to partial culling as determined by an interferon-gamma assay

Sungmo Je, Un-chang Yeo, Taeksun Song, Ki-cheol Kim, Sung-Yun Park, Man-Jung Kim, Sang-Nae Cho* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 12 259-265 (7 pages)

Sungmo Je, Un-chang Yeo, Taeksun Song, Ki-cheol Kim, Sung-Yun Park, Man-Jung Kim, Sang-Nae Cho* 저
...
- 3,000원
The interferon-gamma (IFN-γ) assay is employed as a complementary diagnostic test for bovine tuberculosis (BTB) in many countries. To simplify this assay, we established a 96-well plate format using the ESAT-6 and CFP-10 antigens and then employed it to determine the extent of Mycobacterium (M.) bovis infection in dairy herds with a history of BTB outbreaks in a country where only selective culling is practiced. The sensitivity and specificity of this IFN-γ assay were 85.9% and 100%,...
73.

Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers

Claudia Concha, María Daniella Carretta, Pablo Alarcón, Ivan Conejeros, Diego Gallardo, Alejandra Isabel H 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 7 217-224 (8 pages)

Claudia Concha, María Daniella Carretta, Pablo Alarcón, Ivan Conejeros, Diego Gallardo, Alejandra Isabel H 저
...
- 3,000원
Reactive oxygen species (ROS) production is one of the main mechanisms used to kill microbes during innate immune response. D-lactic acid, which is augmented during acute ruminal acidosis, reduces platelet activating factor (PAF)-induced ROS production and L-selectin shedding in bovine neutrophils in vitro. This study was conducted to investigate whether acute ruminal acidosis induced by acute oligofructose overload in heifers interferes with ROS production and L-selectin shedding in blood...
74.

Development and clinical evaluation of a rapid diagnostic kit for feline leukemia virus infection

Won-Shik Kim, Chom-Kyu Chong, Hak-Yong Kim, Gyu-Cheol Lee, Wooseog Jeong, Dong-Jun An, Hye-Young Jeoung, Jae-In Lee, Young-Ki Le 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 10 91-97 (7 pages)

Won-Shik Kim, Chom-Kyu Chong, Hak-Yong Kim, Gyu-Cheol Lee, Wooseog Jeong, Dong-Jun An, Hye-Young Jeoung, Jae-In Lee, Young-Ki Le 저
...
- 3,000원
Feline leukemia virus (FeLV) causes a range of neoplastic and degenerative diseases in cats. To obtain a more sensitive and convenient diagnosis of the disease, we prepared monoclonal antibodies specific for the FeLV p27 to develop a rapid diagnostic test with enhanced sensitivity and specificity. Among these antibodies, we identified two clones (hybridomas 8F8B5 and 8G7D1) that specifically bound to FeLV and were very suitable for a diagnostic kit. The affinity constants for 8F8B5 and 8G7D1...
75.

Development of a novel enzyme-linked immunosorbent assay to detect anti-IgG against swine hepatitis E virus

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 4호 13 467-472 (6 pages)

Won Jung Lee, Min Kyoung Shin, Seung Bin Cha, Han Sang Yoo 저
...
- 3,000원
a public concern worldwide. Infected pigs are asymptomatic, so diagnosing swine HEV relies on detection of the virus or antibodies against the virus. However, several obstacles need to be overcome for effective and practical serological diagnosis. In this study, we developed an enzyme-linked immunosorbent assay (ELISA) that used a purified recombinant capsid protein of swine HEV. The potential clinical use of this assay was evaluated by comparing it with a commercial kit (Genelabs Technologies,...
76.

A survey of porcine reproductive and respiratory syndrome among wild boar populations in Korea

Eun-Jin Choi, Chang-Hee Lee, Bang-Hun Hyun, Jae-Jo Kim, Seong-In Lim, Jae-Young Song, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 4호 7 377-383 (7 pages)

Eun-Jin Choi, Chang-Hee Lee, Bang-Hun Hyun, Jae-Jo Kim, Seong-In Lim, Jae-Young Song, Yeun-Kyung Shin* 저
...
- 3,000원
No information is currently available on porcine reproductive and respiratory syndrome virus (PRRSV) infection in wild boars (Sus scrofa) in Korea. In this study, the status of PRRS in wild boars was investigated. Blood samples were collected from 267 wild boars from eight provinces in Korea. Four of the samples tested (1.5%) were positive for PRRSV antibodies and eight (3.0%) were positive for antigens. Of the virus-positive samples, three and five samples were typed as containing European (EU,...
77.

Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea

Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 7 43-48 (6 pages)

Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 저
...
- 3,000원
It is essential to rapidly and precisely diagnose rabies. In this study, we evaluated four diagnostic methods, indirect fluorescent antibody test (FAT), virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and rapid immunodiagnostic assay (RIDA), to detect rabies in animal brain homogenates. Out of the 110 animal brain samples tested, 20 (18.2%) were positive for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100,...
78.

Determination of staphylococcal exotoxins, SCCmec types, and genetic relatedness of Staphylococcus intermedius group isolates from veterinary staff, companion animals, and hospital environments in Korea

Jung-Ho Youn, Hye Cheong Koo*, Kuk Ju Ahn, Suk-Kyung Lim, Yong Ho Park* 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 4 221-226 (6 pages)

Jung-Ho Youn, Hye Cheong Koo*, Kuk Ju Ahn, Suk-Kyung Lim, Yong Ho Park* 저
...
- 3,000원
The Staphylococcus (S.) intermedius group (SIG) has been a main research subject in recent years. S. pseudintermedius causes pyoderma and otitis in companion animals as well as foodborne diseases. To prevent SIG-associated infection and disease outbreaks, identification of both staphylococcal exotoxins and staphylococcal cassette chromosome mec (SCCmec) types among SIG isolates may be helpful. In this study, it was found that a single isolate (one out of 178 SIG isolates examined) harbored the...
79.

Molecular characterization of Korean rabies virus isolates

Dong-Kun Yang*, Young-Nam Park, Gyeong-Soo Hong, Hee-Kyung Kang, Yoon-I Oh, Soo-Dong Cho, Jae-Young Song 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 9 57-63 (7 pages)

Dong-Kun Yang*, Young-Nam Park, Gyeong-Soo Hong, Hee-Kyung Kang, Yoon-I Oh, Soo-Dong Cho, Jae-Young Song 저
...
- 3,000원
The nucleoprotein (N) and glycoprotein (G) of 11 Korean rabies virus (RABV) isolates collected from animals diagnosed with rabies between 2008 and 2009 were subjected to molecular and phylogenetic analyses. Six isolates originated from domestic animals (cattle and dogs) and five were obtained from wild free-ranging raccoon dogs. The similarities in the nucleotide sequences of the N gene among all Korean isolates ranged from 98.1 to 99.8%, while those of the G gene ranged from 97.9 to 99.3%....
80.

Antimutagenic and anticarcinogenic effect of methanol extracts of Petasites japonicus Maxim leaves

Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 대한수의학회 Journal of Veterinary Science 8 Pages

대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 8 51-58 (8 pages)

Hwan-Goo Kang, Sang-Hee Jeong*, †, Joon-Hyoung Cho 저
...
- 3,000원
The methanol extract from the leaves of Petasites japonicus Maxim (PJ) was studied for its (anti-)mutagenic effect with the SOS chromotest and reverse mutation assay. The (anti-)carcinogenic effects were evaluated by the cytotoxicity on human cancer line cells and by the function and the expression of gap junctions in rat liver epithelial cell. PJ extracts significantly decreased spontaneous β-galactosidase activity and β-galactosidase activity induced by a mutagen, ICR, in Salmonella (S.)...

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