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791.

Two-dimensional gel electrophoresis and immunoblot analysis of Neospora caninum tachyzoites

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 8 139-145 (7 pages)

Eung-Goo Lee, Jae-Hoon Kim, Yong-Seung Shin, Gee-Wook Shin, Yong-Hwan Kim, Gon-Sup Kim, Dae-Yong Kim, Tae-Sung Jung, Myung-Deuk 저
...
- 3,000원
Identification of expressed protein profiles and antigenic determination are some of the most challenging aspects of proteomics. Two-dimensional gel electrophoresis (2-DE) combined with immunoblot analysis were employed to study the N. caninum proteome. Protein sample preparation was carried out by first conducting sonication, followed by adding lysis buffer containing 7M urea plus 2M thiourea to the purified tachyzoites in order to complete disruption. A total of 335 differentially expressed...
792.

Escherichia coli O157:H7 adherence to HEp-2 cells is implicated with curli expression and outer membrane integrity

Sang-Hyun Kim, Yong-Hwan Kim 대한수의학회 Journal of Veterinary Science 6 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 5 119-124 (6 pages)

Sang-Hyun Kim, Yong-Hwan Kim 저
...
- 3,000원
Escherichia coli (E. coli) has ability to express thin aggregative fimbriae, known as curli, on the cell surface. Previously, a few example of curli expression in serogroup O157:H7 of enterohemorrhagic E. coli (EHEC) were reported, compared to other E. coli groups. However, significance of curliation in the EHEC pathobiology has not been described well in the literature. A highly curliated O157:H7 strain was used in this study in order to elucidate role of curliation in EHEC adherence to...
793.

Comparative antibody response of five recombinant antigens in related to bacterial shedding levels and development of serological diagnosis based on 35 kDa antigen for Mycobacterium avium subsp. Paratuberculosis

Sung Jae Shin, Han Sang Yoo, Sean P. McDonough, Yung-Fu Chang 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 4 111-117 (7 pages)

Sung Jae Shin, Han Sang Yoo, Sean P. McDonough, Yung-Fu Chang 저
...
- 3,000원
this 35 kDa is more accurate indicator of MPT infection than other antigens. At the cut-off point recommended by the ROC curve analysis, the sensitivity and specificity of 35 kDa ELISA were higher than those of other antigens with 93.3% and 86.4%, respectively. Finally, a commercially available ELISA kit was used to clarify 200 positive and 200 negative sera. We then re-tested these serum samples with our ELISA test using the 35-kDa antigens. 35 kDa ELISA and commercial kit showed almost similar...
794.

Pharmacokinetics, tissue residue and plasma protein binding of ofloxacin in goats

Himangshu Baruah, Dulal Chandra Roy, Rohini Kumar Roy, Hirendra Nath Khonikor 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 2 97-101 (5 pages)

Himangshu Baruah, Dulal Chandra Roy, Rohini Kumar Roy, Hirendra Nath Khonikor 저
...
- 1,500원
± 0.15 ?g/ml) at 24 h. The mean AUC, AUMC, t1/2, MRT, Cl and Vd were calculated to be 58.94 ± 19.43 ?g h/ml, 1539.57 ± 724.69 ?g h2/ml, 15.58 ± 1.87 h, 22.46 ± 2.71 h, 135.60 ± 31.12 ml/h/kg and 2.85 ± 0.74 L/kg respectively. Significantly high concentration of drug was detected in different tissues after 24 h of intravenous dosing of 5mg/kg, at 24 h interval for 5 days. The in vitro plasma protein binding of ofloxacin was found to be 15.28 ± 0.94%. Based on these kinetic parameters, a...
795.

An immunohistochemical study of the gastrointestinal endocrine cells in the ddY mice

Sae-kwang Ku, Hyeung-sik Lee, Jae-hyun Lee 대한수의학회 Journal of Veterinary Science 9 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 2호 1 87-95 (9 pages)

Sae-kwang Ku, Hyeung-sik Lee, Jae-hyun Lee 저
...
- 3,000원
The distributions and frequencies of some endocrine cells in the gastrointestinal (GI) tract of ddY mice were studied with immunohistochemical method using 7 types of antisera against bovine chromogranin (BCG), serotonin, gastrin, cholecystokinin (CCK)-8, somatostatin, glucagon and human pancreatic polypeptide (HPP). All of 7 types of immunoreactive (IR) cells were identified. Most of IR cells in the intestinal portion were generally spherical or spindle in shape (open typed cell) while cells...
796.

Optimization of in situ hybridization assay using nonradioactive DNA probes for the detection of canine erpesvirus (CHV) in paraffin-embedded sections

Okjin Kim 대한수의학회 Journal of Veterinary Science 3 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 10 71-73 (3 pages)

Okjin Kim 저
...
- 1,500원
fixed, paraffin embedded sections, which has been used routinely in veterinary fields. Sections of the CHV-infected cell preparation were subjected to several different ISH protocols using digoxigenin- or biotin-labeled probe respectively. Results were compared for the hybridization and background signal intensities. The best result was obtained by the optimized ISH protocol using digoxigenin-labeled probe for detection of CHV DNA. The optimized ISH assay, which developed in this study, may be a...
797.

Mutation and overexpression of p53 as a prognostic factor in canine mammary tumors

Chung-Ho Lee, Wan-Hee Kim, Ji-Hey Lim, Min-Soo Kang, Dae-Yong Kim and Oh-Kyeong Kweon 대한수의학회 Journal of Veterinary Science 7 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 9 63-69 (7 pages)

Chung-Ho Lee, Wan-Hee Kim, Ji-Hey Lim, Min-Soo Kang, Dae-Yong Kim and Oh-Kyeong Kweon 저
...
- 3,000원
3 cases, respectively. p53 mutations were found in 6 cases out of the cases with detected p53 protein expression. The MIB-1 index in benign and malignant tumors were 17.6 ± 20.8% and 29.0 ± 27.2%, respectively and there was no significant difference between tumor types. There was a significant correlation between p53 mutations and p53 overexpression (correlation coefficient = 0.5, p < 0.05). In Kaplan-Meier survival analysis, the p53 index was associated with significantly shortened survival...
798.

Characterization of lymphocyte subpopulations and major histocompatibility complex haplotypes of mastitis-resistant and susceptible cows

Yong Ho Park, Yi Seok Joo, Joo Youn Park, Jin San Moon, So Hyun Kim, Nam Hoon Kwon, Jong Sam Ahn, William C. Davis and Christoph 대한수의학회 Journal of Veterinary Science 11 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 5 29-39 (11 pages)

Yong Ho Park, Yi Seok Joo, Joo Youn Park, Jin San Moon, So Hyun Kim, Nam Hoon Kwon, Jong Sam Ahn, William C. Davis and Christoph 저
...
- 4,000원
Bovine mastitis is an infectious disease with a major economic influence on the dairy industry worldwide. Many factors such as environment, pathogen, and host affect susceptibility or resistance of an individual cow to bovine mastitis. Recently, there has been considerable interest in defining genetic and immunological markers that could be used to select for improved disease resistance. In this study we have analyzed the lymphocyte subpopulations of mastitis-resistant and susceptible cows using...
799.

Inherited canine copper toxicosis in Australian Bedlington Terriers

Changbaig Hyun and Lucio John Filippich 대한수의학회 Journal of Veterinary Science 10 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 4 19-28 (10 pages)

Changbaig Hyun and Lucio John Filippich 저
...
- 3,000원
Inherited copper toxicosis in Bedlington Terriers (CTBT) is a copper associated hepatopathy caused by an autosomal recessive genetic defect of gene involving copper metabolism. To compare clinical and histopathological findings with previous reports and to expand our knowledge for future genetic studies, 18 terriers were clinically and histopathologically examined in this study. Pedigree information and dietary history were obtained from the owners before a thorough clinical examination was...
800.

Assessment of the California mastitis test usage in smallholder dairy herds and risk of violative antimicrobial residues

Anakalo Shitandi and Gathoni Kihumbu 대한수의학회 Journal of Veterinary Science 5 Pages

대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 2 5-9 (5 pages)

Anakalo Shitandi and Gathoni Kihumbu 저
...
- 1,500원
were Staphylococcus aureus (45.6%), coagulase-negative Staphylococci (13.0%), Streptococci (11.7%) and Escherichia coli 5.9%. There was an overall poor but significant (p < 0.05) correlation between the CMT and the violative antimicrobial residues in samples from all quarters, infected and non-infected respectively. The results suggest that the CMT use amongst the smallholder dairy sector as a mastitic indicator may not be a risk factor in violative antimicrobial residues problems in milk.

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