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Research Articles : Soybean mosaic virus Infection and Helper Component-protease Enhance Accumulation of Bean pod mottle virus-Specific siRNAs
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 3 315-323 (9 pages)
Soybean plants infected with Bean pod mottle virus (BPMV) develop acute symptoms that usually decrease in severity over time. In other plant-virus interactions, this type of symptom recovery has been associated with degradation of viral RNAs by RNA silencing, which is accompanied by the accumulation of virus-derived small interfering RNAs (siRNAs). In this study, changes in the accumulation of BPMV siRNAs were investigated in soybean plants infected with BPMV alone, or infected with both BPMV... -
Research Articles : Evaluation of Potential Reference Genes for Quantitative RT-PCR Analysis in Fusarium graminearum under Different Culture Conditions
한국식물병리학회 The Plant Pathology Journal 9 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 1 301-309 (9 pages)
The filamentous fungus Fusarium graminearum is an important cereal pathogen. Although quantitative realtime PCR (qRT-PCR) is commonly used to analyze the expression of important fungal genes, no detailed validation of reference genes for the normalization of qRT-PCR data has been performed in this fungus. Here, we evaluated 15 candidate genes as references, including those previously described as housekeeping genes and those selected from the whole transcriptome sequencing data. By a combination... -
Molecular Characterization of Fusarium Graminearum Virus 2 Isolated from Fusarium graminearum Strain 98-8-60
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 11 285-290 (6 pages)
single open reading frame (ORF). Computer analysis of dsRNA-1 revealed a putative open reading frame (ORF) that shows high sequence identity with an RNA-dependent RNA polymerase (RdRp) containing eight conserved motifs. dsRNAs 2-5 also each contain one putative ORF coding for products of unknown function. The sequences of FgV2 dsRNA-2 and dsRNA-3 have significant sequence identity with Magnaporthe oryzae chrysovirus 1 (MoCV1) dsRNA-3 and -4, respectively. When compared to other dsRNA mycoviruses... -
Occurrence of Tropical Race 4 of Fusarium oxysporum f. sp. cubense in Indonesia
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 10 280-284 (5 pages)
Fusarium wilt of banana caused by Fusarium oxysporum f. sp. cubense is widespread in Indonesia. However, the distribution of tropical race 4 strains has not been well studied. Thirty nine isolates of F. oxysporum f. sp. cubense were collected from Java and 7 isolates were from Sumatera, Bangka, and Kalimantan. All isolates produced volatile odor when grown on steamed rice. These isolates were further tested for their vegetative compatibility with nitM testers of 20 reported vegetative... -
The Road to RNA Silencing is Paved with Plant-Virus Interactions
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 3호 1 197-206 (10 pages)
RNA silencing has had a large impact on biology in general, as well as on our understanding of plantpathogen interactions, especially interactions between plants and viruses. While most of what we know about the mechanism of RNA silencing was deduced in the last 12 years, many of the interactions between plants and viruses, as well as virus-virus interactions in plants, which we now know are manifestations of RNA silencing, were the subject of decades of work from numerous laboratories. These... -
Genetic Reassortment of Rice stripe virus RNA Segments Detected by RT-PCR Restriction Enzyme Analysis-based Method
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 7 148-155 (8 pages)
Our previous sequence and phylogenetic analyses of the Korean Rice stripe virus (RSV) suggested possible genetic reassortment of RNA segments, but whether this RNA variation contributed to the recent RSV outbreaks in Korea is yet unclear. To further clarify these RSVRNA segment variations, we developed a reverse transcription- polymerase reaction/restriction enzyme (RTPCR/ RE) analysis-based method. We identified five REs, including DraI, EcoR1, NdeI/AseI, and SpeI, that could differentiate RSV... -
Diversity of Macrophomina phaseolina Based on Morphological and Genotypic Characteristics in Iran
한국식물병리학회 The Plant Pathology Journal 10 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 5 128-137 (10 pages)
Fifty two Macrophomina phaseolina isolates were recovered from 24 host plant species through the 14 Iranian provinces. All isolates were confirmed to species using species-specific primers. The colony characteristics of each isolate were recorded, including chlorate phenotype, relative growth rate at 30 -
Aggressiveness in Plasmopara halstedii (sunflower downy mildew)
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 2호 2 110-115 (6 pages)
Aggressiveness was studied in seven Plasmopara halstedii (sunflower downy mildew) pathotypes: 100, 300, 304, 314, 704, 710 and 714. Aggressiveness criteria including percentage infection, latent period, sporulation density and reduction of hypocotyl length (dwarfing) were analysed in one sunflower inbred line showing a high level of quantitative resistance. Genetic relationships were detected between the seven pathotypes using 12 ESTderived markers. Pathotypes 100, 300, 304 and 314 were... -
Development of RT-PCR Based Method for Detecting Five Non-reported Quarantine Plant Viruses Infecting the Family Cucurbitaceae or Solanaceae
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 15 93-97 (5 pages)
For quarantine purpose, we selected five plant RNA viruses including Cucumber vein yellowing virus (CVYV), Cucurbit yellow stunting disorder virus (CYSDV), Potato aucuba mosaic virus (PAMV), Potato yellow dwarf virus (PYDV), and Tomato chlorosis virus (ToCV), which are not reported in Korea and cause serious economic losses to the family Cucurbitaceae or Solanaceae. To detect those viruses, we employed RT-PCR technique with specific oligonucleotide primer pairs and tested their detection... -
Outbreak of Powdery Mildew on Zinnia elegans by Golovinomyces cichoracearum in Korea, 2008-2010
한국식물병리학회 The Plant Pathology Journal 4 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 1호 13 85-88 (4 pages)
Podosphaera fusca (syn. Sphaerotheca fusca, S. fuliginea) is a causal agent of powdery mildew infections on Zinnia elegans, and has been documented in Korea since 1967. An outbreak involving about 1,000 plants in October 2008, in Yangpyeong, Korea, was caused by Golovinomyces cichoracearum. Further surveys of zinnia powdery mildew from 2008-2010 confirmed the widespread occurrence of Zinnia-Golovinomyces association in Korea. Chasmothecia of this fungus have not been found on Z. elegans in...


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