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Analysis of the seroprevalence of bovine paratuberculosis and the application of modified absorbed ELISA to field sample testing in Korea
Kun Taek Park, Jongsam Ahn, William C. Davis, Hye Cheong Koo, Nam Hoon Kwon, Woo Kyung Jung, Jun Man Kim, Soon Keun Hong, Yong H 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 8 349-354 (6 pages)
Paratuberculosis (PTB) is a major disease problem worldwide, and causes major economic losses in the dairy industry. Although PTB has been reported in Korea, no studies have been conducted to determine its prevalence and no program has been developed to control the disease. In this study, the sera of beef (n = 1,056) and dairy cattle (n = 1,105) from all provinces in Korea were tested to determine the prevalence of PTB using two different ELISA: an ‘in house’ modified absorbed ELISA (P-ELISA)... -
Gamma-ray irradiation stimulates the expression of caveolin-1 and GFAP in rat spinal cord: a study of immunoblot and immunohistochemistry
Meejung Ahn, Heechul Kim, Jeong Tae Kim, Jeeyoung Lee, Jin Won Hyun, Jae Woo Park, Taekyun Shin 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 4호 1 309-314 (6 pages)
1 PI (p < 0.05), and returned to the level of normal control rats on days 4 and 9 PI. Immunohistochemistry showed that caveolin-1 immunoreactivity was enhanced in some glial cells, vascular endothelial cells, and neurons in the spinal cords. The increased expression of glial fibrillary acidic protein (GFAP), a marker for an astroglial reaction, was consistent with that of caveolin-1. In addition, caveolin-1 was co-localized in hypertrophied GFAP-positive astrocytes. Taking all these facts into... -
Neuropharmacological effects of deltamethrin in rats
S. Manna, D. Bhattacharyya, T. K. Mandal, S. Dey 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 7 133-136 (4 pages)
sleeping time and pentylenetetrazole (PTZ)-induced convulsion as well as the gamma aminobutyric acid (GABA) level. Albino Wistar rats were used as the experimental animals. Different neuropharmacological paradigms such as the motor co-ordination by the rotarod, pentobarbitone-induced sleeping time and the PTZinduced convulsion were examined after administering deltamethrin orally at two doses, 150 mg/kg (LD50) and 15 mg/kg (1/10 LD50). The GABA level in the rat brain was estimated by HPLC after... -
Excitability scores of goats administered ascorbic acid and transported during hot-dry conditions
J. O. Ayo, N. S. Minka, M. Mamman1 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 6 127-131 (5 pages)
In this study, we investigated the effect of ascorbic acid (AA) administration on goat excitability due to transportation. Ten goats administered AA (p.o.) at 100 mg/kg of body weight before transportation served as the experimental group, and seven goats administered only 10 ml/kg of sterile water (p.o.) served as controls. Excitability scores were recorded for each goat; when weighed, before, immediately after, and 3 h after 8 h of transportation. A score of one to four was allocated to each... -
Immunohistochemical study of caveolin-1 and -2 in the rat retina
Heechul Kim, Taeki Lee, Jeeyoung Lee, Meejung Ahn, Changjong Moon, Myung Bok Wie, Taekyun Shin 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 2호 1 101-104 (4 pages)
The expression of caveolin-1 and -2 in the retina was examined; Western blot analysis showed that both were present. Immunohistochemistry indicated that caveolin-1 was expressed in the majority of retinal layers, including the ganglion cell layer, inner plexiform layer, outer plexiform layer, and in the vascular endothelial cells of the retina. Caveolin-2 was primarily immunostained in the vessels, but in a few other elements as well. This is the first demonstration of caveolin differential... -
Practical tips for modified dorsolumbar epidural anesthesia in cattle
Inhyung Lee, Norio Yamagishi, Kenji Oboshi, Naoki Sasaki, Haruo Yamada 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 12 69-72 (4 pages)
Segmental dorsolumbar epidural anesthesia has been considered difficult to perform. The purpose of this study was to determine whether or not it is difficult for beginners to learn how to do modified dorsolumbar epidural anesthesia of cattle. Thirty cattle were divided into two groups, young (n = 8) and adult (n = 22), according to their age and body weight, and 0.12% new methylene blue (NMB) was injected into the first interlumbar (L1?L2) epidural space by four fifth-year veterinary school... -
Primary survey of avian influenza virus and Newcastle disease virus infection in wild birds in some areas of Heilongjiang Province, China
Yu Ping Hua, Hong Liang Chai, Si Yuan Yang, Xiang Wei Zeng, Ying Sun 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2005, 제 6권 제 4호 9 311-315 (5 pages)
Two hundred thirty specimens of wild birds were collected from some areas in Heilongjiang Province during the period of 2003~2004, including two batches of specimens collected randomly from a same flock of mallards in Zhalong Natural Reserve in August and December, 2004, respectively. Primary virus isolation and identification for avian influenza virus (AIV) and Newcastle disease virus (NDV) were performed. The results showed that only two specimens of young mallards collected from Zhalong... -
Inherited canine copper toxicosis in Australian Bedlington Terriers
Changbaig Hyun and Lucio John Filippich 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2004, 제 5권 제 1호 4 19-28 (10 pages)
Inherited copper toxicosis in Bedlington Terriers (CTBT) is a copper associated hepatopathy caused by an autosomal recessive genetic defect of gene involving copper metabolism. To compare clinical and histopathological findings with previous reports and to expand our knowledge for future genetic studies, 18 terriers were clinically and histopathologically examined in this study. Pedigree information and dietary history were obtained from the owners before a thorough clinical examination was... -
Immunohistochemistry of Voltage-Gated Calcium Channel α1B Subunit in Mouse Cerebellum
Seung-Hyuk Chung, Kyung-Youl Lee, Ki-Hyung Kim, Chul-Tae Kim, Nam-Seob Lee, Kazuhiko Sawada, Hiromi Haga, Byung-Chul Lee, Yoshih 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 3호 5 175-178 (4 pages)
Secretion of neurotransmitters is initiated by voltage-gated calcium influx through presynaptic, voltage- gated N-type calcium channels. However, little is known about their cellular distribution in the mouse cerebellum. In the cerebellum, alpha1B immunoreactivity is found mainly on the cell bodies of all Purkinje cells. In addition, the immunoreactivity was detected on a subset of Purkinje cell dendrites, clustered to form a parasagittal array of bands. In the anterior lobe vermis,... -
Expression of Open Reading Frame 5 Protein of Porcine Reproductive and Respiratory Syndrome Virus Using Semliki Forest Virus Expression System
Hyun Soo Kim, Kwang Soon Shin, In Wook Hwang, Chul Joong Kim, Su Mi Kim, Hae Sun Jung 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2002, 제 3권 제 1호 3 13-18 (6 pages)
The ORF5 gene encodes a major envelope glycoprotein (GP5), which is one of the three major proteins of porcine reproductive and respiratory syndrome virus (PRRSV). The GP5 protein has been known to be a 24.5-26 kDa N-glycosylated envelope protein. The GP5 is involved in inducing neutralizing antibodies. For this reason, the GP5 is primary candidate for the PRRSV subunit vaccine. To produce the native form of GP5 in mammalian cells, we have cloned the ORF5 gene from PRRSV CNV-1 into the Semliki...


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