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Improved development of somatic cell cloned bovine embryos by a mammary gland epithelia cells in vitro model
Xiao-ying He, Li-bing Ma, Xiao-ning He, Wan-tong Si, Yue-Mao Zheng 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2016, 제 17권 제 2호 3 145-152 (8 pages)
Previous studies have established a bovine mammary gland epithelia cells in vitro model by the adenovirus-mediated telomerase (hTERT-bMGEs). The present study was conducted to confirm whether hTERT-bMGEs were effective target cells to improve the efficiency of transgenic expression and somatic cell nuclear transfer (SCNT). To accomplish this, a mammary-specific vector encoding human lysozyme and green fluorescent protein was used to verify the transgenic efficiency of hTERT-bMGEs, and untreated... -
Magnetic nanoparticle based purification and enzyme-linked immunosorbent assay using monoclonal antibody against enrofloxacin
Nam-Gun Kim, Myeong-Ae Kim, Young-Il Park, Tae-Sung Jung, Seong-Wan Son, ByungJae So, Hwan-Goo Kang 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 4호 6 431-437 (7 pages)
Monoclonal anti-enrofloxacin antibody was prepared for a direct competitive enzyme-linked immunosorbent assay (ELISA) and purification system using monoclonal antibody (mAb) coupled magnetic nanoparticles (MNPs). The IC50 values of the developed mAb for enrofloxacin (ENR), ciprofloxacin, difloxacin, sarafloxacin, pefloxacin, and norfloxacin were 5.0, 8.3, 9.7, 21.7, 36.0, and 63.7 ng/mL, respectively. The lowest detectable level of ENR was 0.7 ng/mL in the prepared ELISA system. To validate the... -
Osteogenic potential of mesenchymal cells derived from canine umbilical cord matrix co-cultured with platelet-rich plasma and demineralized bone matrix
Talita F.B. Souza, Silmara S. Sakamoto, Gabriel T.N.M. Ferreira, Roberto Gameiro, Marcia Marinho, Alexandre L. de Andrade, Terez 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 17 381-384 (4 pages)
Canine mesenchymal cells (MSCs) derived from Wharton’s jelly were co-cultured, then supplemented or not supplemented with platelet rich plasma (PRP) and demineralized bone matrix (DBM) to verify osteogenic differentiation. Osteoblastic differentiation followed by mineralized bone matrix production was found to be significantly higher (p < 0.05) when MSCs were associated with PRP/DBM in culture after 14–21-days of induction. Osteopontin and osteocalcin gene expression were... -
Factors affecting the success of resynchronization protocols with or without progesterone supplementation in dairy cows
Annette Forro , Georgios Tsousis, Nicola Beindorff, Ahmad Reza Sharifi, Christos Brozos, Heinrich Bollwein 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 16 121-126 (6 pages)
The objective of this study was to investigate factors that influence the success of resynchronization protocols for bovines with and without progesterone supplementation. Cow synchronized and not found pregnant were randomly assigned to two resynchronization protocols: ovsynch without progesterone (P4) supplementation (n = 66) or with exogenous P4 administered from Days 0 to 7 (n = 67). Progesterone levels were measured on Days 0 and 7 of these protocols as well as 4... -
Canine model of ischemic stroke with permanent middle cerebral artery occlusion: clinical features, magnetic resonance imaging, histopathology, and immunohistochemistry
Joon-Hyeok Jeon, Hae-Won Jung, Hyo-Mi Jang, Jong-Hyun Moon, Ki-Tae Park, Hee-Chun Lee, Ha-Young Lim, Jung-Hyang Sur, By 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 10 75-85 (11 pages)
clinical, MRI, histopathologic, and immunohistochemical findings associated with ischemic stroke in dogs. Additionally, the association of cerebrospinal fluid (CSF) and tissue levels of interleukin (IL)-6 with clinical prognosis was assessed. Ischemic stroke was induced by permanent middle cerebral artery occlusion (MCAO) in nine healthy experimental dogs. The dogs were divided into three groups according to survival time and duration of the experimental period: group... -
Autologous processed plasma: cytokine profile and effects upon injection into healthy equine joints
Juliana J. Moreira, Ana Paula L. Moraes, Patricia M. Brossi, Thais S.L. Machado, Yara M. Michelacci, Cristina O. Massoc 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 1호 7 47-55 (9 pages)
joints. The effects on joints were analysed with a short-phase protocol and a prolonged-phase protocol using saline-injected joints as controls. For the short protocol, horses received one intra-articular APP injection. Synovial fluid samples were collected prior to the injection and 3, 6, 24, 48, and 16 h after treatment. For the prolonged protocol, the joints received three weekly injections of APP, and samples were collected at 0, 7, 14, 21, and 28 days before APP... -
Enhancing mucosal immunity in mice by recombinant adenovirus expressing major epitopes of porcine circovirus-2 capsid protein delivered with cytosine-phosphate-guanosine oligodeoxynucleotides
Hong-tao Chang, Xiu-yuan He, Yu-Feng Liu, Lu Chen*, Quan-hai Guo, Qiu-ying Yu, Jun Zhao, Xin-wei Wang, Xia Yang, Chuan-qing Wang 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 8 399-407 (9 pages)
A recombinant replication-defective adenovirus expressing the major epitopes of porcine circovirus-2 (PCV-2) capsid protein (rAd/Cap/518) was previously constructed and shown to induce mucosal immunity in mice following intranasal delivery. In the present study, immune responses induced by intranasal immunization with a combination of rAd/Cap/518 and cytosine-phosphate-guanosine oligodeoxynucleotides (CpG ODN) were evaluated in mice. The levels of PCV-2-specific IgG in serum and IgA in saliva,... -
Effect of superovulation on uterine and serum biochemical parameters and its potential association with transferable embryos in Holstein dairy cows
Hasina Santatriniaina Rasolomboahanginjatovo, Younès Chorfi*, Raynald Dupras, Louis Mills, Réjean Lefebvre 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 14 273-281 (9 pages)
The objective of this study was to determine the effects of superovulation (SOV) on serum and uterine biochemical parameters, uterine bacteriology and cytology and number of transferable embryos (TE). Dairy cows were placed on a Presynch/CIDR Synch protocol. The SOV group was superovulated, induced in estrus, and inseminated, whereas the control group was induced in estrus and inseminated without SOV. Uterine bacteriology and cytology and uterine and serum biochemical parameters were measured at... -
Oxidative response of neutrophils to platelet-activating factor is altered during acute ruminal acidosis induced by oligofructose in heifers
Claudia Concha, María Daniella Carretta, Pablo Alarcón, Ivan Conejeros, Diego Gallardo, Alejandra Isabel H 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 7 217-224 (8 pages)
samples were collected using rumenocentesis. Lactic acid from plasma and ruminal samples was measured by HPLC. PAF-induced ROS production and L-selectin shedding were measured in vitro in bovine neutrophils by a luminol chemiluminescence assay and flow cytometry, respectively. A significant increase in ruminal and plasma lactic acid was recorded in these animals. Specifically, a decrease in PAF-induced ROS production was observed 8 h after oligofructose overload, and this was sustained until 48... -
Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine
Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)
The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR)....


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