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본문검색 결과만 보기
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The signal sequence of type II porcine reproductive and respiratory syndrome virus glycoprotein 3 is sufficient for endoplasmic reticulum retention
Do-Geun Kim, Chang-Seon Song, In-Soo Choi, Seung-Yong Park, Joong-Bok Lee, Sang-Soo Lee 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 10 307-313 (7 pages)
The glycoprotein 3 (GP3) of type II porcine reproductive and respiratory syndrome virus has the characteristic domains of a membrane protein. However, this protein has been reported to be retained in the endoplasmic reticulum (ER) rather than transported to the plasma membrane of the cell. In this study, we performed confocal laser scanning microscopy analysis of variants of GP3 and foundthat the signal sequence of the GP3 led to confinement of GP3 in the ER, while the functional ortransmembrane... -
Preparation and diagnostic utility of a hemagglutination inhibition test antigen derived from the baculovirus-expressed hemagglutinin-neuraminidase protein gene of Newcastle disease virus
Kang-Seuk Choi, Soo-Jeong Kye, Woo-Jin Jeon, Mi-Ja Park, Saeromi Kim, Hee-Jung Seul, Jun-Hun Kwon 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 8 291-297 (7 pages)
A recombinant hemagglutinin-neuraminidase (rHN) protein from Newcastle disease virus (NDV) with hemagglutination (HA) activity was expressed in Spodoptera frugiperda cells using a baculovirus expression system. The rHN protein extracted from infected cells was used as an antigen in a hemagglutination inhibition (HI) test for the detection and titration of NDV-specific antibodies present in chicken sera. The rHN antigen produced high HA titers of 2 per 25 μL, which were similar to those of the... -
Mitochondrial and DNA damage in bovine somatic cell nuclear transfer embryos
In-Sun Hwang, Hyo-Kyung Bae, Hee-Tae Cheong 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 3호 1 235-240 (6 pages)
The generation of reactive oxygen species (ROS) and subsequent mitochondrial and DNA damage in bovine somatic cell nuclear transfer (SCNT) embryos were examined. Bovine enucleated oocytes were electrofused with donor cells and then activated by a combination of Ca-ionophore and 6-dimethylaminopurine culture. The H2O2 and ·OH radical levels, mitochondrial morphology and membrane potential (ΔΨ), and DNA fragmentation of SCNT and in vitro fertilized (IVF) embryos at the zygote stage were... -
Genetic diversity of porcine reproductive and respiratory syndrome virus in Korea
Eun-Jin Choi, Chang-Hee Lee, Jae-Young Song, Hee-Jong Song, Choi-Kyu Park, Byounghan Kim, Yeun-Kyung Shin* 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 2호 2 115-124 (10 pages)
PRRSV while 198 farms were positive for type 2. Nucleotide sequences encoding the open reading frame (ORF) 5 were analyzed and compared to those of various published PRRSV isolates obtained worldwide. Sequence identity of the ORF 5 in the isolates was 81.6∼100% for type 1 viruses and 81.4∼100% for type 2 viruses. Phylogenetic analysis of the ORF 5 sequences showed that types 1 and 2 PRRSVs from Korea were mainly classified into three and four clusters, respectively. The analyzed isolates were... -
Pulsed tissue Doppler imaging of the left ventricular septal mitral annulus in healthy dogs
Jihye Choi, Hyunwook Kim, Junghee Yoon* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 12 85-90 (6 pages)
This study evaluated pulsed TDI variables including the isovolumic time interval and duration of the major wave in a population of large healthy dogs. Longitudinal myocardial motion at the septal mitral annulus was evaluated with pulsed TDI in 45 healthy adult dogs. Maximal myocardial velocities, isovolumic time intervals, and duration of the myocardial waves were measured. The correlation between time intervals and velocity variables was also investigated. The mean maximal systolic velocity was... -
Expression and lytic efficacy assessment of the Staphylococcus aureus phage SA4 lysin gene
Anil Kumar Mishra*, Mayank Rawat, Konasagara Nagaleekar Viswas, Abhishek, Sujeet Kumar, Manjunatha Reddy 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 6 37-43 (7 pages)
a single 802-bp open reading frame encoding a partial protein with a calculated mass of 30 kDa. Results of this analysis also indicated that the LysSA4 sequence shared a high homology with endolysin of the GH15 phage and other reported phages. The LysSA4 gene of the SA4 phage was subsequently expressed in Escherichia coli. Recombinant LysSA4 induced the lysis of host bacteria in a spot inoculation test, indicating that the protein was expressed and functionally active. Furthermore, recombinant... -
Induction of humoral responses to BHV-1 glycoprotein D expressed by HSV-1 amplicon vectors
Andrea Maria Blanc, Mabel Beatriz Berois, Lorena Magalí Tomé, Alberto L. Epstein, Juan Ramón Arbiza* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 9 59-65 (7 pages)
in the infected cells, the ability of both vectors to induce a specific gD immune response was tested in BALB/c mice that were intramuscularly immunized. Specific serum antibody responses were detected in mice inoculated with both vectors, and the response against truncated gD was higher than the response against full-length gD. These results reinforce previous findings that HSV-1 amplicon vectors can potentially deliver antigens to animals and highlight the prospective use of these vectors for... -
Comparison of four diagnostic methods for detecting rabies viruses circulating in Korea
Dong-Kun Yang*, Eun-Kyung Shin, Yoon-I Oh, Kyung-Woo Lee, Chung-San Lee, Seo-Young Kim, Jeong-A Lee, Jae-Young Song 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 7 43-48 (6 pages)
It is essential to rapidly and precisely diagnose rabies. In this study, we evaluated four diagnostic methods, indirect fluorescent antibody test (FAT), virus isolation (VI), reverse transcriptase polymerase chain reaction (RT-PCR), and rapid immunodiagnostic assay (RIDA), to detect rabies in animal brain homogenates. Out of the 110 animal brain samples tested, 20 (18.2%) were positive for rabies according to the FAT. Compared to the FAT, the sensitivities of VI, RT-PCR, and RIDA were 100, 100,... -
Molecular characterization of a 13-amino acid deletion in VP1 (1D) protein and novel amino acid substitutions in 3D polymerase protein of foot and mouth disease virus subtype A/Iran87
Majid Esmaelizad*, Saber Jelokhani-Niaraki, Khadije Hashemnejad, Morteza Kamalzadeh, Mohsen Lotfi 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 9 363-371 (9 pages)
The nucleotide sequence of the VP1 (1D) and partial 3D polymerase (3D) coding regions of the foot and mouth disease virus (FMDV) vaccine strain A/Iran87, a highly passaged isolate (∼150 passages), was determined and aligned with previously published FMDV serotype A sequences. Overall analysis of the amino acid substitutions revealed that the partial 3D coding region contained four amino acid alterations. Amino acid sequence comparison of the VP1 coding region of the field isolates revealed... -
Evaluation of the Perkins handheld applanation tonometer in horses and cattle
Silvia Franco Andrade, Daniel Silva Kupper, Luiz Fernando Rodrigues de Pinho, Elizabeth Cunha Franco, Marcus Vin?cius Felix Fabr 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 10 171-176 (6 pages)
The objective of this study was to evaluate and validate the accuracy of the Perkins handheld applanation tonometer for measuring intraocular pressure (IOP) in horses and cattle. Both eyes of 10 adult horses and cattle were evaluated in a postmortem study. The eyes from 10 clinically normal adult horses and cattle were also examined after bilateral auriculopalpebral nerve block and topical anesthesia for an in vivo study. IOP was measured postmortem using direct manometry (measured with an...
