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Pathological characterization of TgElk mice injected with brain homogenate from elk with chronic wasting disease
Yong-Chul Jeon, Jin-Kyu Choi, Eun-Kyung Choi, Richard I. Carp, Yong-Sun Kim* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2013, 제 14권 제 1호 4 21-26 (6 pages)
Chronic wasting disease (CWD) is classified as a transmissible spongiform encephalopathy or prion disease that affects cervids. CWD has been reported in 15 US states, two Canadian provinces, and in imported elk on several farms in Korea. This study was conducted to examine the molecular biological and pathogenic characteristics of a CWD-associated prion isolated in Korea. The epidemiological origin of this pathogen was also determined. Homozygous TgElk mice were infected with a CWD-affected elk... -
Genotypic characterization of Staphylococcus aureus isolated from bovines, humans, and food in Indonesia
Siti Isrina Oktavia Salasia*, Syarifudin Tato, Ngalijan Sugiyono, Dwi Ariyanti, Feny Prabawati 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 8 353-361 (9 pages)
S. aureus was based on culturing and biochemical tests, and by amplifying a specific section of the 23S rRNA gene. PCR amplification of the SE genes (sea, seb, sec, see, seg, seh, and sei) singly or in combination was observed. Most isolates of bovine origin harbored hla (84%) and cap5 (74%), while most isolates from humans harbored hla (73%), cap8 (91%), and fnbA (100%). Strains from food sources were positive for hla (100%), cap5 (100%), and cap8 (64%) unlike isolates from humans or bovines. A... -
Molecular characterization of tetracycline- and quinolone-resistant Aeromonas salmonicida isolated in Korea
Ji Hyung Kim, Sun Young Hwang, Jee Soo Son, Jee Eun Han, Jin Woo Jun, Sang Phil Shin, Casiano Choresca Jr, Yun Jaie Choi, Yong H 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 7 41-48 (8 pages)
The antibiotic resistance of 16 Aeromonas (A.) salmonicida strains isolated from diseased fish and environmental samples in Korea from 2006 to 2009 were investigated in this study. Tetracycline or quinolone resistance was observed in eight and 16 of the isolates, respectively, based on the measured minimal inhibitory concentrations. Among the tetracycline-resistant strains, seven of the isolates harbored tetA gene and one isolate harbored tetE gene. Additionally, quinolone-resistance determining... -
Infrared spectroscopy characterization of normal and lung cancer cells originated from epithelium
So Yeong Lee, Kyong-Ah Yoon, Soo Hwa Jang, Erdene Ochir Ganbold, Dembereldorj Uuriintuya, Sang-Mo Shin, Pan Dong Ryu, Sang-Woo J 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 4호 4 299-304 (6 pages)
The vibrational spectral differences of normal and lung cancer cells were studied for the development of effective cancer cell screening by means of attenuated total reflection infrared spectroscopy. The phosphate monoester symmetric stretching ns(PO3) band intensity at ∼970 cm and the phosphodiester symmetric stretching ns(PO2) band intensity at ∼1,085 cm in nucleic acids and phospholipids appeared to be significantly strengthened in lung cancer cells with respect to the other... -
Genetic characterization of Barbari goats using microsatellite markers
J. Ramamoorthi, K. Thilagam, S. N. Sivaselvam*, S. M. K. Karthickeyan 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2009, 제 10권 제 1호 11 73-76 (4 pages)
a part of genetic characterization and conservation. The genomic DNA from 50 unrelated Barbari goats were amplified via PCR with a panel of 21 microsatellite markers, and resolved through 6 per cent denaturing polyacrylamide gel electrophoresis followed by silver staining. The number of alleles ranged from 4 to 11, with allele sizes ranging from 88 to 220 bp. The distribution of allele frequencies was between 0.0104 and 0.5208. Polymorphism information content varied from 0.5563 to 0.8348. The... -
Molecular characterization and genogrouping of VP1 of aquatic birnavirus GC1 isolated from rockfish Sebastes schlegeli in Korea
Seong Joon Joh, *, Chae Ik Shon, Sung Won Kang, Byoung Han Kim, Byung Yul Jeong, Kyung Gi Lee, Jun Hun Kwon, Gang Jun Heo2 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2008, 제 9권 제 1호 11 85-90 (6 pages)
The cDNA nucleotide sequence of genome segment B encoding the VP1 protein was determined for the aquatic birnavirus GC1 isolated from the rockfish Sebastes schlegeli in Korea. The VP1 protein of GC1 contains a 2,538 bp open reading frame, which encodes a protein comprising 846 amino acid residues that has a predicted MW of 94 kDa. The sequence contains 6 potential Asn-X-Ser/Thr motifs. Eight potential Ser phosphorylation sites and 1 potential Tyr phophorylation site were also identified. GC1... -
Purification and characterization of two larval glycoproteins from the cattle tick, Boophilus annulatus
Amr E. El Hakim, Yasser E. Shahein, Amira M. K. Abouelella, Mohamed E. Selim 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2007, 제 8권 제 2호 12 175-180 (6 pages)
The present study was conducted to identify new target immunogenic molecules from the larval stage of the cattle tick, Boophilus annulatus (Acari: Ixodidae). Two specific larval glycoproteins (GLPs) were isolated by two-step affinity chromatography. The larval immunogens were first purified with CNBr-Sepharose coupled to rabbit anti-larval immunoglobulins, and the glycoproteins were then purified with Con-A Sepharose. These glycoproteins have molecular weights of approximately 32 and 15 kDa with... -
The 23S rRNA gene PCR-RFLP used for characterization of porcine intestinal spirochete isolates
Tae Jung Kim, Jae Il Lee 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 11 277-280 (4 pages)
Using three reference strains of Brachyspira hyodysenteriae (B204, B234, B169), one B. pilosicoli (P43/6/78), one B. murdochii (56-150), one B. intermedia (PWS/A), one B. innocens (B256) and ten Korean isolates, PCR-RFLP analysis of DNA encoding 23S rRNA was performed to establish a rapid and accurate method for characterizing porcine intestinal spirochetes. Consequently, B. hyodysenteriae and B. pilosicoli revealed different restriction patterns; however, the other three species shared the same... -
Characterization of HC58cDNA, a putative cysteine protease from the parasite Haemonchus contortus
Charles I. Muleke, Yan Ruofeng, Xu Lixin, Sun Yanming, Li Xiangrui 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 3호 7 249-255 (7 pages)
the biological and biochemical characteristics of individual CBL genes is lacking. In this study, we investigated the degradative effects of the recombinant HC58 protein isolated from Haemonchus contortus parasites on protein substrates over a broad pH range in vitro. This protein, which hydrolyzed the synthetic peptide substrates Z-FR-AMC and Z-RR-AMC, had characteristics of the cysteine protease class of proteins. In the acidic pH range, the isolated protein actively degraded hemoglobin (Hb),... -
Isolation and characterization of Streptococcus sp.from diseased flounder (Paralichthys olivaceus )in Jeju Island
Gun Wook Baeck, Ji Hyung Kim, Dennis Kaw Gomez, Se Chang Park 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2006, 제 7권 제 1호 9 53-58 (6 pages)
samples were identified as Lactococcus garvieae and 18 samples were identified as Streptococcus parauberis by culture-based, biochemical test.Serological methods such as slide agglutination, hemolysis and antimicrobial susceptibility test were also used as well as multiplex PCR- based method to simultaneously detect and confirm the pathogens involved in the infection.S.parauberis and L. garvieae have a target region of 700 and 1100 bp., respectively.One fish sample was not identified because of...
