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Alpha basic crystallin expression in canine mammary tumors
Tolga Guvenc*, Mustafa Yavuz Gulbahar, Murat Yarim, Yonca Betil Kabak, Onder Karayigit, Mahmut Sozmen 대한수의학회 Journal of Veterinary Science 4 Pages
대한수의학회 Journal of Veterinary Science 2012, 제 13권 제 1호 6 39-42 (4 pages)
The aim of this study was to evaluate prognostic and/or diagnostic factors of canine mammary tumors by immunohistochemically analyzing the expression of alpha basic crystallin (aB-c). For this, formalin-fixed, paraffin-embedded blocks of 51 naturally-occurring canine mammary tumors (11 benign and 40 malignant) were used. Tissue from eight normal canine mammary glands were served as a control. Immunohistochemically, in the control mammary tissues, a few luminal epithelial cells were aB-c positive... -
Expression and regulation of Enpp2 in rat uterus during the estrous cycle
Hyo-Jin Ahn, Hyun Yang, Beum-Soo An, Kyung-Chul Choi, Eui-Bae Jeung* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 11 379-385 (7 pages)
Ectonucleotide pyrophosphatase/phosphodiestrase 2 (Enpp2) isolated from the supernatant of human melanoma cells is a lysophospholipase D that transforms lysophosphatidylcholine into lysophospatidic acid. Although multiple analyses have investigated the function of Enpp2 in the hypothalamus, its role in the uterus during the estrous cycle is not well understood. In the present study, rat uterine Enpp2 was analyzed by RT-PCR, Western blotting, and immunohistochemistry. Quantitative PCR analysis... -
Butyrate regulates leptin expression through different signaling pathways in adipocytes
Mohamed Mohamed Soliman*, Mohamed Mohamed Ahmed, Alaa-eldin Salah-eldin, Abeer Abdel-Alim Abdel-Aal 대한수의학회 Journal of Veterinary Science 5 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 4호 3 319-323 (5 pages)
Leptin is an adipocytokine that regulates body weight, and maintains energy homeostasis by promoting reduced food intake and increasing energy expenditure. Leptin expression and secretion is regulated by various factors including hormones and fatty acids. Butyrate is a short-chain fatty acid that acts as source of energy in humans. We determined whether this fatty acid can play a role in leptin expression in fully differentiated human adipocytes. Mature differentiated adipocytes were incubated... -
Influence of nitric oxide on in vitro growth, survival, steroidogenesis, and apoptosis of follicle stimulating hormone stimulated buffalo (Bubalus bubalis) preantral follicles
Pawan K. Dubey, Vrajesh Tripathi, Ram Pratap Singh, G. Taru Sharma* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 3호 9 257-265 (9 pages)
Effect of sodium nitroprusside (SNP), a nitric oxide (NO) donor, on in vitro survival, growth, steroidogenesis, and apoptosis of buffalo preantral follicles (PFs) was investigated. PFs (200∼250 mm) were isolated by micro-dissection and cultured in 0 (control), 10, 10, 10, and 10 M SNP. To examine the reversible effect of SNP, PFs were cultured with 10 M SNP + 1 mM N-nitro-L-arginine methyl ester (L-NAME) or 1.0 mg hemoglobin (Hb). The results showed that greater concentrations of SNP (10,... -
The stress of weaning influences serum levels of acute-phase proteins, iron-binding proteins, inflammatory cytokines, cortisol, and leukocyte subsets in Holstein calves
Myung-Hoo Kim, Ji-Young Yang, Santi Devi Upadhaya, Hyun-Jun Lee, Cheol-Heui Yun, Jong K. Ha 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 7 151-158 (8 pages)
The purpose of our study was to investigate changes in immunological parameters induced by weaning stress (including milk restriction) in calves. Fifteen Holstein calves were subjected to weaning at 6 weeks of age. Blood samples were collected at -14, -7, -2, 1, 3, and 5 days post-weaning (DPW; 0 DPW = 42 days). Weaning caused significant (p < 0.01) increases in the neutrophil (NE):lymphocyte (LY) ratio at 5 DPW with a significant (p < 0.05) reduction of LYs. The concentration of acute-phase... -
Dynamic analysis of Ca level during bovine oocytes maturation and early embryonic development
Su Li Liang, Qian Jun Zhao, Xiang Chen Li, Ya Ping Jin, Yi Peng Wang, Xiao Hua Su, Wei Jun Guan, Yue Hui Ma 대한수의학회 Journal of Veterinary Science 10 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 2호 5 133-142 (10 pages)
Mammalian oocyte maturation and early embryo development processes are Ca-dependent. In this study, we used confocal microscopy to investigate the distribution pattern of Ca and its dynamic changes in the processes of bovine oocytes maturation, in vitro fertilization (IVF), parthenogenetic activation (PA) and somatic cell nuclear transfer (SCNT) embryo development. During the germinal vesicle (GV) and GV breakdown stage, Ca was distributed in the cortical ooplasm and throughout the oocytes from... -
The expressions of HSP70 and αB-crystallin in myocarditis associated with foot-and-mouth disease virus in lambs
Mustafa Yavuz Gulbahar*, Yonca Betil Kabak, Mehmet Onder Karayigit, Murat Yarim, Tolga Guvenc, Unal Parlak 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2011, 제 12권 제 1호 10 65-73 (9 pages)
This study describes the expression of heat shock protein70 (HSP70) and alpha-basic-crystallin (α-BC) and their association with apoptosis and some related adaptor proteins in the pathogenesis of foot-and-mouth disease virus (FMDV)-induced myocarditis in lambs. HSP70 was generally overexpressed in the myocardial tissues and inflammatory cells of FMDV-induced myocarditis with differential accumulation and localization in same hearts when compared to non-foot-and-mouth disease control hearts.... -
Immunomodulatory effects of betulinic acid from the bark of white birch on mice
Jin-e Yi, Bozena Obminska-Mrukowicz, Li-yun Yuan, Hui Yuan* 대한수의학회 Journal of Veterinary Science 9 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 5 305-313 (9 pages)
The objective of this study was to explore the immunomodulatory effects of betulinic acid (BA) extracted from the bark of white birch on mice. Female mice were orally administered BA for 14 days in doses of 0, 0.25, 0.5, and 1 mg/kg body weight. We found that BA significantly enhanced the thymus and spleen indices, and stimulated lymphocyte proliferation induced by Concanavalin A and lipopolysaccharide as shown by MTT assay. Flow cytometry revealed that BA increased the percentage of CD4 cells... -
Expression of KA1 kainate receptor subunit in the substantia gelatinosa of the trigeminal subnucleus caudalis in mice
Seon Ah Park, Soo Joung Park, Seong Kyu Han* 대한수의학회 Journal of Veterinary Science 6 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 4호 4 299-304 (6 pages)
The KA1 kainate receptor (KAR) subunit in the substantia gelatinosa (SG) of the trigeminal subnucleus caudalis (Vc) has been implicated in the processing of nociceptive information from the orofacial region. This study compared the expression of the KA1 KAR subunit in the SG of the Vc in juvenile, prepubescent and adult mice. RT-PCR, Western blot and immunohistochemistry analyses were used to examine the expression level in SG area. The expression levels of the KA1 KAR subunit mRNA and protein... -
Ethanol extract of Angelica gigas inhibits croton oil-induced inflammation by suppressing the cyclooxygenase-prostaglandin pathway
Sunhee Shin, Seong Soo Joo, Dongsun Park, Jeong Hee Jeon, Tae Kyun Kim, Jeong Seon Kim, Sung Kyeong Park, Bang Yeon Hwang, *, Yun 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2010, 제 11권 제 1호 7 43-50 (8 pages)
The anti-inflammatory effects of an ethanol extract of Angelica gigas (EAG) were investigated in vitro and in vivo using croton oil-induced inflammation models. Croton oil (20 μg/mL) up-regulated mRNA expression of cyclooxygenase (COX)-I and COX-II in the macrophage cell line, RAW 264.7, resulting in the release of high concentrations of prostaglandin E2 (PGE2). EAG (1∼10 μg/mL) markedly suppressed croton oil-induced COX-II mRNA expression and PGE2 production. Application of croton oil (5% in...


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