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전체 선택해제
총 161건 검색
본문검색 결과만 보기
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Transcriptome Analysis of Early Responsive Genes in Rice during Magnaporthe oryzae Infection
한국식물병리학회 The Plant Pathology Journal 12 Pages
한국식물병리학회 The Plant Pathology Journal 2014, 30권 4호 2 343-354 (12 pages)
Rice blast disease caused by Magnaporthe oryzae is one of the most serious diseases of cultivated rice (Oryza sativa L.) in most rice-growing regions of the world. In order to investigate early response genes in rice, we utilized the transcriptome analysis approach using a 300 K tilling microarray to rice leaves infected with compatible and incompatible M. oryzae strains. Prior to the microarray experiment, total RNA was validated by measuring the differential expression of rice defense-related... -
Research Notes : Transcriptome Analysis of the Small Brown Planthopper, Laodelphax striatellus Carrying Rice stripe virus
한국식물병리학회 The Plant Pathology Journal 8 Pages
한국식물병리학회 The Plant Pathology Journal 2013, 29권 3호 12 330-337 (8 pages)
Rice stripe virus (RSV), the type member of the genus Tenuivirus, transmits by the feeding behavior of small brown planthopper (SBPH), Laodelphax striatellus. To investigate the interactions between the virus and vector insect, total RNA was extracted from RSV-viruliferous SBPH (RVLS) and non-viruliferous SBPH (NVLS) adults to construct expressed sequence tag databases for comparative transcriptome analysis. Over 30 million bases were sequenced by 454 pyrosequencing to construct 1,538 and 953 of... -
Population Structure of the Gibberella fujikuroi Species Complex Associated with Rice and Corn in Korea
한국식물병리학회 The Plant Pathology Journal 7 Pages
한국식물병리학회 The Plant Pathology Journal 2012, 28권 4호 2 357-363 (7 pages)
Several species belonging to the Gibberella fujikuroi species (Gf) complex are commonly associated with rice and corn, not only causing serious diseases, but also producing fumonisins, a group of mycotoxins harmful to animals and humans. To characterize the population structure of the putative fumonisin-producing Gf complex in Korea, we obtained 276 candidate isolates from rice and corn harvested in 2009 and 2010 by diagnostic polymerase chain reaction with several specific primer sets.... -
Research Articles : Effect of Rice stripe virus NS3 on Transient Gene Expression and Transgene Co-Silencing
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2011, 27권 4호 2 310-314 (5 pages)
Nonstructural protein 3 (NS3) encoded by RNA3 of Rice stripe virus (RSV), known to be a suppressor of gene silencing, was cloned and sequenced. The cloned NS3 gene is composed of 636 nucleotides encoding 211 deduced amino acids, and showed a high degree of similarity with the equivalent genes isolated from Korea, Japan and China. The NS3 gene promoted the enhancement of transient gene expression and suppressed transgene co-silencing. In the transient GFP expression via agroinfiltration, GFP... -
Reverse Transcription Droplet Digital PCR을 활용한 Tomato Spotted Wilt Virus 검출 및 정량
이효정, 박기범, 한연수, 정래동 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2021, 27권 3호 5 120-127 (8 pages)
식물 바이러스는 작물 수확량에 상당한 손실을 일으키고 작 물 생산을 지속적으로 위협하여 세계 식량 안보에 심각한 위협 이 된다. 그 중 tomato spotted wilt virus (TSWV)는 주로 원예 작물을 감염시키는 가장 위협적인 식물 바이러스로 넓은 기주 범위를 가진다. Reverse-transcription quantitative real-time PCR (RT-qPCR)은 TSWV의 민감한 검출을 위해 널리 사용되 고 있지만 표준화의 어려움으로 인해 유용성이 감소한다. 따라 서 본 연구에서는 TSWV 검출을 위해 민감하고 정확한 reverse transcription droplet digital... -
Identification of Differentially Expressed Genes by cDNA-AFLP in Magnaporthe oryzae
Myoung-Hwan Chi, Sook-Young Park 한국식물병리학회 식물병연구 8 Pages
한국식물병리학회 식물병연구 2019, 25권 4호 7 205-212 (8 pages)
Analysis of differentially expressed genes has assisted discovery of gene sets involved in particular biological processes. The purpose of this study was to identify genes involved in appressorium formation in the rice blast fungus Magnaporthe oryzae via analysis of cDNA–amplified fragment length polymorphisms. Amplification of appressorial and vegetative mycelial cDNAs using 28 primer combinations generated over 200 differentially expressed transcript-derived fragments (TDFs). TDFs were...
