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Caffeine and 2-Aminoethoxydiphenyl Borate (2-APB) Have Different Ability to Inhibit Intracellular Calcium Mobilization in Pancreatic Acinar Cell
KyungJinChoi, KabSungKim, SeHoonKim, DongKwanKim, HyungSeoPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2010, Vol.14 No.2 7 105-111 (7 pages)
the mouse pancreatic acinar cell. Caffeine did not block the Ca2＋ entry, but significantly inhibited carbamylcholine (CCh)-induced Ca2＋ release. In contrast, 2-APB did not block CCh-induced Ca2＋ release, but remarkably blocked SOC-mediated Ca2＋ entry at lower concentrations. In permeabilized acinar cell, caffeine had an inhibitory effect on InsP3-induced Ca2＋ release, but 2-APB at lower concentration, which effectively blocked Ca2＋ entry, had no inhibitory action. At higher concentrations,... -
The effect of intravenous injection of human adipose derived stem cell conditioned medium on ovulation and subsequent embryo development in aged mice
Kihae Ra, Geon A Kim, Hyun Ju Oh, Sung Keun Kang, Jeong Chan Ra, Byeong Chun Lee 대한수의학회 대한수의학회 학술대회발표집 2 Pages
대한수의학회 대한수의학회 학술대회발표집 추계학술심포지움 316 381-382 (2 pages)
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Hydrogen peroxide attenuates refilling of intracellular calcium store in mouse pancreatic acinar cells
MiNaYoon, DongKwanKim, SeHoonKim, HyungSeoPark 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2017, Vol.21 No.2 11 233-239 (7 pages)
of pancreatic acinar cells. Reactive oxygen species are known to be associated with a variety of oxidative stress-induced cellular disorders including pancreatitis. In this study, we investigated the effect of hydrogen peroxide (H2O2) on intracellular Ca2+ accumulation in mouse pancreatic acinar cells. Perfusion of H2O2 at 300 μM resulted in additional elevation of intracellular Ca2+ levels and termination of oscillatory Ca2+ signals induced by carbamylcholine (CCh) in the presence of normal... -
Activation of K+ channel by 1-EBIO rescues the head and neck squamous cell carcinoma cells from Ca2+ ionophore-induced cell death
MingZheYin, Seok-WooPark, , TaeWookKang, KyungSooKim, , , HaeYoungYoo6, JunhoLee, J.HunHah, , MyungHunSung, , SungJoonKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 9 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2016, Vol.20 No.1 4 25-33 (9 pages)
investigate the role of Ca2+-activated K+ channel (SK4) in head and neck squamous cell carcinoma cells (HNSCCs) of dif-ferent cell lines; SNU-1076, OSC-19 and HN5. Treatment with 1 μM ionomycin induced cell death in all the three cell lines. Whole-cell patch clamp study suggested common expressions of Ca2+-activated Cl− channels (Ano-1) and Ca2+-activated nonselective cation channels (CAN). 1-EBIO, an activator of SK4, induced outward K+ current (ISK4) in SNU-1076 and OSC-19. In HN5, ISK4... -
Ca2+ is a Regulator of the WNK/OSR1/NKCC Pathway in a Human Salivary Gland Cell Line
SoonhongPark, SangKyunKu, HyeWonJi, Jong-HoonChoi, DongMinShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 7 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2015, Vol.19 No.3 9 249-255 (7 pages)
Wnk kinase maintains cell volume, regulating various transporters such as sodium-chloride cotransporter, potassium-chloride cotransporter, and sodium-potassium-chloride cotransporter 1 (NKCC1) through the phosphorylation of oxidative stress responsive kinase 1 (OSR1) and STE20/SPS1-related proline/alanine-rich kinase (SPAK). However, the activating mechanism of Wnk kinase in specific tissues and specific conditions is broadly unclear. In the present study, we used a human salivary gland (HSG)... -
Role of Regulators of G-Protein Signaling 4 in Ca2＋ Signaling in Mouse Pancreatic Acinar Cells
SoonhongPark, Syng-IllLee, DongMinShin 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2011, Vol.15 No.6 9 383-388 (6 pages)
acinar cells. Moreover, deletion of RGS4 increased the frequency of Ca2＋ oscillations. RGS4−/− cells also showed increased expression of sarco/endoplasmic reticulum Ca2＋ ATPase type 2. However, there were no significant alterations, such as Ca2＋ signaling in treated high dose of agonist and its related amylase secretion activity, in acinar cells from RGS4−/− mice. These results indicate that RGS4 protein regulates Ca2＋ signaling in mouse pancreatic acinar cells. -
Altered Gene Expression in Cerulein-Stimulated Pancreatic Acinar Cells: Pathologic Mechanism of Acute Pancreatitis
JiHoonYu, JooWeonLim, HyeyoungKim 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 8 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2009, Vol.13 No.6 1 409-416 (8 pages)
of digestive enzymes. The genes expressed in pancreatic acinar cells determine the severity of the disease. The present study determined the differentially expressed genes in pancreatic acinar cells treated with cerulein as an in vitro model of acute pancreatitis. Pancreatic acinar AR42J cells were stimulated with 10−8 M cerulein for 4 h, and genes with altered expression were identified using a cDNA microarray for 4,000 rat genes and validated by real-time PCR. These genes showed a... -
MAPK Activation and Cell Viability after H2O2 Stimulation in Cultured Feline Ileal Smooth Muscle Cells
HyunJuSong, JiHoonJeong, DongKyuLee, TaiSangLee, YoungSilMin, UyDongSohn 대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 6 Pages
대한생리학회-대한약리학회 The Korean Journal of Physiology & Pharmacology 2004, Vol.8 No.6 8 339-344 (6 pages)
cultured ileal smooth muscle cells (ISMC) after stimulation with H2O2. Trypan blue method revealed that the cell viability of ISMC treated with 1 mM H2O2 was not different from that of controls at up to 2 h time point, while treatment of ISMC with 1 mM H2O2 for 48 h finally induced significant decrease in the cell viability. Therefore, we evaluated whether H2O2 was capable of ERKs activation in ISMC for the short-term exposure and examined whether tyrosine kinase was involved in the process of...
