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Profiling of Disease-Related Metabolites in Grapevine Internode Tissues Infected with Agrobacterium vitis
한국식물병리학회 The Plant Pathology Journal 11 Pages
한국식물병리학회 The Plant Pathology Journal 2016, 32권 6호 1 489-499 (11 pages)
Green shoot cuttings of 10 different grapevine species were inoculated with Agrobacterium vitis to find disease-related metabolites in the grapevine. Crown galls formed 60 days after inoculation varied in gall severity (GS) evaluated by gall incidence (GI) and gall diameter (GD), which were classified into three response types as RR (low GI and small GD), SR (high GI and small GD), and SS (high GI and large GD), corresponding to resistant, moderately resistant, and susceptible responses,... -
Establishment of an Agrobacterium-mediated Inoculation System for Cucumber Green Mottle Mosaic Virus
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2015, 31권 4호 13 433-437 (5 pages)
by Agrobacterium-mediated infiltration. Virulence and symptomatic characteristics of the cloned CGMMV isolates KW and KOM were tested on several indicator plants. No obvious differences between two cloned isolates in disease development were observed on the tested indicator plants. We also determined full genome sequences of the cloned CGMMV isolates KW and KOM. Sequence comparison revealed that only four amino acids (at positions 228, 699, 1212, and 1238 of the replicase protein region) differ... -
Distribution of Agrobacterium tumefaciens Biovars in Jordan and Variation of Virulence
한국식물병리학회 The Plant Pathology Journal 5 Pages
한국식물병리학회 The Plant Pathology Journal 2006, 22권 4호 3 318-322 (5 pages)
One hundred and nine Agrobacterium isolates were recovered from 68 samples (51 plant tumor and 17 soil) that were collected from different habitats in Northern Jordan. The isolated cultures were grouped into 3 biovars based on their biochemical characteristics and biovar I, II, and III comprised a total number of 46, 41, and 22 isolates, respectively. Isolates of biovar I were obtained primarily from the diseased peach, oak and rose plants, whereas isolates of biovar II and III were obtained... -
Antifungal Gene (Rs-AFP) Introduction into Rehmannia glutinosa and Gene Expression Mediated by Agrobacterium tumefaciens
한국식물병리학회 The Plant Pathology Journal 6 Pages
한국식물병리학회 The Plant Pathology Journal 2003, 19권 1호 10 51-56 (6 pages)
plants by using Agrobacterium tumefaciences and AFP (antifungal protein) gene. The plant material used was a native accession of R. glutinosa. The PCR analysis was conducted to verify transgenicity. Based on the PCR analysis, nptII band was observed in transgenic plant genome. Southern blot and AFP protein analyses also showed the expression of this gene in transgenic plants. Expression of AFP in transgenic plants offers the possibility of developing resistance to fungal infection. -
충북지방의 뿌리혹병 감염 포도나무 뿌리에서 분리한 Agrobacterium속 균의 특성
양승업, 박세정, 이영기, 차재순 한국식물병리학회 식물병연구 6 Pages
한국식물병리학회 식물병연구 2009, 15권 2호 5 77-82 (6 pages)
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재조합 Agrobacterium tumefaciens NTL4 균주를 이용한 quorum Sensing Autoinducer 검색에 용매와 계면활성제가 미치는 영향
고경표, 김연희, 김정선, 박성훈, Koh. Kyong-Pyo, Kim. Yeon-Hee, Kim. Jung Sun, Park. Sunghoon 한국해양바이오학회 한국해양바이오학회지 8 Pages
한국해양바이오학회 한국해양바이오학회지 2006, Vol.1 No.4 260-267 (8 pages)
재조합 Agrobacterium tumefaciens균주를 사용한 Liquid Culture Assay는 quorum sensing autoinducer를 검색하는 생물학적 분석 방법으로 개발되었다. 그러나 이 시스템에 사용되는 해양천연물 시료들이 일반적으로 낮은 수용액에 대한 용해도를 갖기 때문에 활성검색에 걸림돌이 되고 있다. 시료의 용해도는 유기용매 혹은 계면활성제의 첨가로 증가될 수 있으나, 유기용매나 계면활성제 자체가 검색결과의 정확한 해석을 방해할 수 있으므로 적절한 조건의 확립이 매우 중요하다. Methanol, ethanol, 1-propanol, DMSO와 DMF를 0~10%...


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