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The Effect of DTT in Protein Preparations for Proteomic Analysis: Removal of a Highly Abundant Plant Enzyme, Ribulose Bisphosphate Carboxylase/Oxygenase
Cho. Jin-Hwan, Hwang. Hee-Youn, Cho. Man-Ho, Kwon. Yong-Kook, Jeon. Jong-Seong, Bhoo. Seong-Hee, Hahn. Tae-Ryong 한국식물학회 Journal of plant biology 5 Pages
한국식물학회 Journal of plant biology 2008, Vol.51 No.4 297-301 (5 pages)
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Comparative Study of Enzyme Activity and Stability of Bovine and Human Plasmins in Electrophoretic Reagents, β-mercaptoethanol, DTT, SDS, Triton X-100, and Urea
Choi. Nack-Shick, Hahm. Jeung-Ho, Maeng. Pil-Jae, Kim. Seung-Ho 생화학분자생물학회 Journal of biochemistry and molecular biology 5 Pages
생화학분자생물학회 Journal of biochemistry and molecular biology 2005, Vol.38 No.2 177-181 (5 pages)
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붓다의 교육원리와 수기적(隨機的) 교수법-진리와 방편의 역동적 연관성을 중심으로
한국종교교육학회 종교교육학연구 2007, 제 25권 4 1-27 (27 pages)
본 논고는 불교의 창시자 고오타마 붓다의 교육체계에 있어서 방편시설과 맥락적 가르침(contextual teaching)의 원리와 방법에 대한 고찰을 통하여 붓다의 교육방법의 현대 교육적 의미를 밝히는 데 목적이 있다. 고오타마 붓다가 인류의 ‘스승(sattha)’이 될 수 있었던 것은 무엇보다도 그의 교육방법의 수승함에 있었다. 교사로서의 붓다는 ‘교육은 방법(marga)이다, 라는 명제를 실천 으로 보여주었다. 붓다의 교육체계에 있어서 방법은 그 내용과 서로 떨어질 수 없는 역동적 상호 연관성이 있었다. 붓다의 교육방법은 그 주제와... -
Effects of ball kicking dual task training on gait performance and balance in individuals with chronic hemiparetic stroke
Min-seong Kim, Jae-hun Shim, Kyung-hoon Yu, Ji-won Kim 물리치료재활과학회 Physical Therapy Rehabilitation Science 8 Pages
물리치료재활과학회 Physical Therapy Rehabilitation Science 2016, 제 5권 제 4호 2 170-177 (8 pages)
each group: ball kicking dual task training (DTT) group and TGT group. The DTT group received ball kicking DTT with cognitive tasks consisted of three stages and the TGT group received TGT using normal walking speed, respectively, for 30minutes per day 3 days per week for 4 weeks. Outcome assessments were made with the 10-meter walking test (10MWT), 6-minute walking test (6MWT), functional gait assessment (FGA), Berg balance scale (BBS), timed up and go test (TUG), and the activities- specific... -
Comparative proteomic analysis of proteins expression changes in the mammary tissue of cows infected with Escherichia coli mastitis
Xiao-wei Zhao, Yong-xin Yang, Dong-wei Huang, Guang-long Cheng, Hui-ling Zhao 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2015, 제 16권 제 3호 2 253-263 (11 pages)
Cows infected with Escherichia (E.) coli usually experience severe clinical symptoms, including damage to mammary tissues, reduced milk yield, and altered milk composition. In order to investigate the host response to E. coli infection and discover novel markers for mastitis treatment, mammary tissue samples were collected from healthy cows and bovines with naturally occurring severe E. coli mastitis. Changes of mammary tissue proteins were examined using two-dimensional gel... -
Proteomic analysis of chicken peripheral blood mononuclear cells after infection by Newcastle disease virus
Xiaoyu Deng, Yanlong Cong, Renfu Yin, Guilian Yang, Chan Ding, Shengqing Yu, Xiufan Liu, Chunfeng Wang, †, Zhuang Ding, 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 4호 7 511-517 (7 pages)
Characteristic clinical manifestations of Newcastle disease include leukopenia and immunosuppression. Peripheral blood mononuclear cells (PBMCs) are the main targets of Newcastle disease virus (NDV) infection. To survey changes in proteomic expression in chicken PBMCs following NDV infection, PBMC proteins from 30 chickens were separated using two- dimensional electrophoresis (2-DE) and subjected to mass spectrometry analysis. Quantitative intensity analysis showed that the expression of 78... -
Antibiotic resistance and molecular characterization of ophthalmic Staphylococcus pseudintermedius isolates from dogs
Min-Hee Kang, Min-Joo Chae, Jang-Won Yoon, Seung-Gon Kim, So-Young Lee, Jong-Hyun Yoo, Hee-Myung Park* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 9 409-415 (7 pages)
The prevalence, virulence potential, and antibiotic resistance of ophthalmic Staphylococcus pseudintermedius (SP) isolated from dogs were examined. Sixty-seven Staphylococcus species were isolated from ophthalmic samples and surveyed for species-specific sequences in the Staphylococcus intermedius group (SIG) nuclease gene (SInuc), exfoliative toxin gene for SIG (siet), and antibiotic resistance genes (blaZ and mecA). PCR-restriction fragment length polymorphism analysis of the pta gene was also... -
Comparison of arylalkylamine N-acetyltransferase and melatonin receptor type 1B immunoreactivity between young adult and aged canine spinal cord
Ji Hyeon Ahn, Joon Ha Park, In Hye Kim, Jae-Chul Lee, Bing Chun Yan, Min Sik Yong, Choong Hyun Lee, Jung Hoon Choi, Ki-Yeon Yoo, 대한수의학회 Journal of Veterinary Science 8 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 3호 1 335-342 (8 pages)
Melatonin affects diverse physiological functions through its receptor and plays an important role in the central nervous system. In the present study, we compared immunoreactivity patterns of arylalkylamine N-acetyltransferase (AANAT), an enzyme essential for melatonin synthesis, and melatonin receptor type 1B (MT2) in the spinal cord of young adult (2∼3 years) and aged (10∼12 years) beagle dogs using immunohistochemistry and Western blotting. AANAT-specific immunoreactivity was observed in... -
Identification of abnormal gene expression in bovine transgenic somatic cell nuclear transfer embryos
Jongki Cho, Sungkeun Kang, Byeong Chun Lee* 대한수의학회 Journal of Veterinary Science 7 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 2호 8 225-231 (7 pages)
This study was conducted to investigate the expression of three genes related to early embryonic development in bovine transgenic cloned embryos. To accomplish this, development of bovine transgenic somatic cell nuclear transfer (SCNT) embryos was compared with non-transgenic embryos. Next, mRNA transcription of three specific genes (DNMT1, Hsp 70.1, and Mash2) related to early embryo development in transgenic SCNT embryos was compared between transgenic and non-transgenic SCNTs, parthenogenetic... -
Positive effects of porcine IL-2 and IL-4 on virus-specific immune responses induced by the porcine reproductive and respiratory syndrome virus (PRRSV) ORF5 DNA vaccine in swine
Deyuan Tang*, Jian Liu, Chunyan Li, Hua Zhang, Ping Ma, Xianfeng Luo, Zhiyong Zeng, Nining Hong, Xia Liu, Bin Wang, Feng Wang, Z 대한수의학회 Journal of Veterinary Science 11 Pages
대한수의학회 Journal of Veterinary Science 2014, 제 15권 제 1호 11 99-109 (11 pages)
The purpose of this study was to investigate the effects of porcine interleukin (IL)-2 and IL-4 genes on enhancing the immunogenicity of a porcine reproductive and respiratory syndrome virus ORF5 DNA vaccine in piglets. Eukaryotic expression plasmids pcDNA-ORF5, pcDNA-IL-2, and pcDNA-IL-4 were constructed and then expressed in Marc-145 cells. The effects of these genes were detected using an indirect immunofluorescent assay and reverse transcription polymerase chain reaction (RT-PCR)....


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